Protective effect of recombinant human mitsugumin 53 on CCl<sub>4</sub>-induced acute liver injury in mice

Zhongshu ZHOU; Zhengfan GONG; Donghai YANG; Lianpan WU; Tianyang XIA; Jinjuan FU; Yukai LIU; Yu HAN; Chunyu ZENG

doi:10.16016/j.1000-5404.201708123

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Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice

Basic Medicine | 更新时间：2026-01-09

- Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice
- Journal of Third Military Medical University Vol. 39, Issue 23, Pages: 2270-2275(2017)
- 作者机构：
  
  400042 重庆，第三军医大学大坪医院野战外科研究所心血管内科，重庆市心血管病研究所
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scientists of China(81600585)
- DOI：10.16016/j.1000-5404.201708123
  CLC： R322.47;R341;R575
- Received：20 August 2017，
  
  Revised：2017-09-28，
  
  Published：15 December 2017
- 稿件说明：
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Zhongshu ZHOU, Zhengfan GONG, Donghai YANG, et al. Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice[J]. Journal of Third Military Medical University, 2017, 39(23): 2270-2275.
DOI：

Zhongshu ZHOU, Zhengfan GONG, Donghai YANG, et al. Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice[J]. Journal of Third Military Medical University, 2017, 39(23): 2270-2275. DOI： 10.16016/j.1000-5404.201708123.

摘要

目的

研究重组人MG53(rhMG53)对四氯化碳(CCl

)诱导的急性肝损伤的保护作用及其相关机制。

方法

40只雄性C57小鼠采用完全随机化分组法分成4组:正常对照组、rhMG53组、CCl

组、rhMG53+CCl

组，每组10只。CCl

组、rhMG53+CCl

组小鼠予0.1% CCl

花生油溶液2 mL/kg腹腔内注射，rhMG53+CCl

组在注射CCl

前30 min予rhMG53 1 mg/kg肌肉注射。rhMG53组予rhMG53 1 mg/kg肌肉注射。正常进食进水24 h后眼球取血分离血清，测定谷丙转氨酶(ALT)、谷草转氨酶(AST)、乳酸脱氢酶(LDH)活性。取小鼠肝脏

观察肝脏的组织学改变。以体外培养的人肝癌细胞(HepG2)为靶细胞，观察rhMG53对CCl

诱导的细胞损伤的保护作用。

结果

rhMG53降低小鼠血清ALT、AST、LDH水平，病理镜检显示rhMG53可改善肝脏的病理结构，差异均有统计学意义(

0.05)；rhMG53可以修复HepG2细胞膜，保护CCl

对HepG2细胞的损伤。

结论

rhMG53改善CCl

诱导的急性肝细胞损伤，保护肝脏功能。

Abstract

Objective

To determine the protective effect of recombinant human MG53 (rhMG53) on acute liver injury in mice induced by carbon tetrachloride (CCl

)

and investigate its underlying mechanism.

Methods

Male C57 mice (

=40) were randomly divided into 4 groups (

=10)

that is

normal control group

rhMG53 group

CCl

group

and rhMG53+CCl

group. The animals of the CCl

group and rhMG53+CCl

group were injected intraperitoneally with 0.1% CCl

peanut oil solution at a dose of 2 mL/kg

and the mice of the latter group were injected intramuscularly with 1 mg/kg rhMG53 in 0.5 h before

CCl

injection. In 24 h after normal feeding

blood samples were collected after eyeball extirpating

and the serum levels of alanine aminotransferase (ALT)

aspartate aminotransferase (AST) and lactate dehydrogenase (LDH) were measured. The liver tissues of the mice were also harvested for histological changes of the livers. Human hepatocellular carcinoma cells (HepG2) were used as target cells to observe the protective effect of rhMG53 on CCl

-induced cell injury.

Results

RhMG53 treatment reduced serum ALT

AST and LDH levels

and significant attenuated the pathological injuries induced by CCl

(

0.05). rhMG53 repaired cell membrane and reduced CCl

-induced damage to HepG2 cells.

Conclusions

rhMG53 shows protective effect on CCl

-induced acute hepatocellular injury and protects the liver function in mice.

关键词

Keywords

references

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⁰

Protective effect of recombinant human mitsugumin 53 on CCl4-induced acute liver injury in mice

DOI：10.16016/j.1000-5404.201708123

摘要

Abstract

关键词

Keywords

references

Protective effect of recombinant human mitsugumin 53 on CCl₄-induced acute liver injury in mice