Preparation and properties of a novel zinc oxide nanoparticles/bacterial cellulose composites

Zhenghui LUO; Hai LIN; Meifang YIN; Yuguang ZHONG; Jie LIU; Yuan WANG; Jun WU; Jiaping ZHANG; Xingdong ZHANG

doi:10.16016/j.1000-5404.201707193

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Preparation and properties of a novel zinc oxide nanoparticles/bacterial cellulose composites

Basic Medicine | 更新时间：2026-01-09

- Preparation and properties of a novel zinc oxide nanoparticles/bacterial cellulose composites
- Journal of Third Military Medical University Vol. 39, Issue 23, Pages: 2250-2254(2017)
- 作者机构：
  
  1.400038 重庆，第三军医大学西南医院全军烧伤研究所，创伤、烧伤与复合伤国家重点实验室
  2.610064 成都，四川大学国家生物医学材料工程技术研究中心
- 作者简介：
- 基金信息：
  
  the Special Project of Application Technological Development and Demonstration Promotion of Hainan Province(SQ2014ZDXM0294);the Major Technological Innovative Program of Southwest Hospital of Third Military Medical University(SWH2016ZDX4201)
- DOI：10.16016/j.1000-5404.201707193
  CLC： R318.08;R641
- Received：29 July 2017，
  
  Revised：2017-09-19，
  
  Published：15 December 2017
- 稿件说明：
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Zhenghui LUO, Hai LIN, Meifang YIN, et al. Preparation and properties of a novel zinc oxide nanoparticles/bacterial cellulose composites[J]. Journal of Third Military Medical University, 2017, 39(23): 2250-2254.
DOI：

Zhenghui LUO, Hai LIN, Meifang YIN, et al. Preparation and properties of a novel zinc oxide nanoparticles/bacterial cellulose composites[J]. Journal of Third Military Medical University, 2017, 39(23): 2250-2254. DOI： 10.16016/j.1000-5404.201707193.

摘要

目的

制备一种新型纳米氧化锌/细菌纤维素复合膜，评价其物理特性和抗菌性能。

方法

采用二步法制备纳米氧化锌/细菌纤维素复合膜。对细菌纤维素膜(bacterial cellulose membrane，BCM)进行羧基化修饰，然后以此为模板，进行纳米氧化锌(zinc oxide nanoparticles，nZnO)的原位合成，制备成nZnO/BCM复合膜；以BCM为对照，扫描电子显微镜观察nZnO/BCM复合膜的形态及nZnO分布；原子吸收光谱法测定nZnO的含量，评价经高压灭菌和浸提处理后nZnO/BCM复合膜中nZnO的结合稳定性；水蒸气透过率检测评价nZnO/BCM复合膜的透气性；万能材料测试仪检测nZnO/BCM复合膜的抗张强度、断裂伸长率及杨氏模量，评价其力学性能；振荡抑菌实验评价nZnO/BCM对金黄色葡萄球菌和大肠杆菌的抑制作用。

结果

电镜观察结果显示，二步法合成的nZnO/BCM复合膜的纤维网络呈三维立体结构，其孔隙大小和密度均显著大于单纯BCM；原位合成的nZnO附着于纤维，呈串珠状排列，nZnO直径为30~90 nm。对nZnO/BCM复合膜(含40% nZnO)进行高压灭菌和浸提处理后，其nZnO的脱失率为8.98%。nZnO/BCM复合膜(含40% nZnO)的水蒸气透过率、抗张强度和杨氏模量均显著高于BCM(

0.05)。nZnO/BCM复合膜(含40% nZnO)对金黄色葡萄球菌和大肠杆菌的抑菌率分别为96.72%、60.14%，显著高于BCM组(

0.05)。

结论

二步法制备的nZnO/BCM复合膜具有良好的孔隙率和nZnO结合稳定性，其透气性和物理力学性能得到进一步提高，且对金黄色葡萄球菌和大肠杆菌具有较好的抗菌活性。

Abstract

Objective

To prepare a new wound dressing based on zinc oxide/bacterial cellulose membranes (nZnO/BCM) and determine its properties and antibacterial activity.

Methods

The assembly of nZnO/BCM was achieved by carboxylation of BCM and

in situ

synthesis of ZnO nanop

articles using the carboxylated BCM as a template in a non-aqueous polar medium. The nZnO/BCM structure was observed by scanning electron microscopy. The stability of nZnO/BCM was measured by atomic absorption spectrometry. The tensile strength

elongation at break and Young modulus of nZnO/BCM were measured by universal material tester. And the permeability of nZnO/BCM was evaluated by the detection of water vapor transmission. The antibacterial activity of nZnO/BCM was tested using shaking flask method on

Staphylococcus aureus

and

Escherichia coli

Results

Our prepared nZnO/BCM had a three-dimensional lattice structure with uniform distribution of ZnO nanospheres in higher density and larger pores when compared with simple BCM. The

in situ

synthesized nZnO (30-90 nm in diameter) bound to the BCM in beaded arrangement. After the nZnO/BCM (containing 40% nZnO) was autoclaved and leached

8.98% of nZnO was released. Water vapor transmission rate

tensile strength and Young's modulus of nZnO/BCM composite membranes (containing 40% nZnO) were significantly higher than those of BCM (

0.05). The inhibitory rates of nZnO/BCM (containing 40% nZnO) against

Staphylococcus aureus

and

Escherichia coli

were 96.72% and 60.14%

respectively

significantly higher than those of BCM group (

0.05).

Conclusions

nZnO/BCM not only possess the uniform distribution of nanoparticles

stable binding

good mechanical properties and proper permeability

but also exhibit good antibacterial activity against

Staphylococcus aureus

and

Escherichia coli

关键词

Keywords

references

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