Efficiency of methazolamide for hypoxia tolerance in mice and its prevention for acute mountain sickness

Gang XU; He HUANG; Dewei CHEN; Libin GUAN; Binda SUN; Qiu HU; Chunhua JIANG; Yongjun LUO; Fuyu LIU; Yuqi GAO

doi:10.16016/j.1000-5404.201707166

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Efficiency of methazolamide for hypoxia tolerance in mice and its prevention for acute mountain sickness

Basic Medicine | 更新时间：2026-01-09

- Efficiency of methazolamide for hypoxia tolerance in mice and its prevention for acute mountain sickness
- Journal of Third Military Medical University Vol. 40, Issue 1, Pages: 12-16(2018)
- 作者机构：
  
  1.400038 重庆, 第三军医大学高原军事医学系:高原特需药品与卫生装备研究室
  2.400038 重庆, 第三军医大学高原军事医学系:病理生理学与高原病理学教研室
  3.400038 重庆, 第三军医大学高原军事医学系:全军高原医学重点实验室
- 作者简介：
- 基金信息：
  
  the National Training Fund for Basic Science Talents(J1310001)
- DOI：10.16016/j.1000-5404.201707166
  CLC： R594.3;R969.4;R983.1
- Received：25 July 2017，
  
  Revised：2017-08-31，
  
  Published：15 January 2018
- 稿件说明：
移动端阅览
Gang XU, He HUANG, Dewei CHEN, et al. Efficiency of methazolamide for hypoxia tolerance in mice and its prevention for acute mountain sickness[J]. Journal of Third Military Medical University, 2018, 40(1): 12-16.
DOI：

Gang XU, He HUANG, Dewei CHEN, et al. Efficiency of methazolamide for hypoxia tolerance in mice and its prevention for acute mountain sickness[J]. Journal of Third Military Medical University, 2018, 40(1): 12-16. DOI： 10.16016/j.1000-5404.201707166.

摘要

目的

研究醋甲唑胺(Methazolamide

MTZ)的抗缺氧效应及其在预防急性高原反应中的作用。

方法

小鼠灌胃给予MTZ后

通过密闭缺氧实验、心肌缺氧实验、大脑缺血缺氧实验、亚硝酸钠中毒实验

分别检测小鼠密闭缺氧状态下的标准耐受时间、小鼠心肌缺氧标准耐受时间、断头处死后张口动作持续时间、亚硝酸钠中毒后存活时间等指标。在高原现场比较志愿者口服MTZ或安慰剂后

急性高原病的发病率、血氧饱和度及心率。

结果

MTZ 400、200、100 mg/kg组的小鼠密闭缺氧状态下的标准耐受时间均显著高于对照组[25.01(2.40)、21.11(4.64)、16.39(5.14)

14.62(3.62) min/100 mL

0.05

]

。同时

200 mg/kg的MTZ还可以显著延长小鼠心肌缺氧标准耐受时间[(13.08±1.35)min/100 mL

(8.30±0.94) min/100 mL

0.01

]

和断头处死后张口动作持续时间(43.22±8.23) s

(20.44±2.60) s

0.01

]

。人群试验表明MTZ显著降低急性高原病的发病率(19.4%

3.45%

0.05)

并显著提高急进高原人群的血氧饱和度[85%(4%)

87%(3.5%)

0.05

]

降低心率[100(17)/min

90(21.5)/min

0.05

]

。

结论

MTZ可显著提高机体抗缺氧能力

能有效预防急性高原病。

Abstract

Objective

To study the anti-hypoxic effect of methazolamide (MTZ) and its prevention of acute high altitude reaction.

Methods

After the mice were intragastrically infused with MTZ

closed hypoxia experiment

myocardial hypoxia experiment

cerebral ischemia and hypoxia experiment and sodium nitrite poisoning experiment were used to determine the survival time in closed hypoxia

myocardial hypoxia tolerance time

mouth movement time after decapitation

and survival time of sodium nitrite poisoning.After the volunteers who took MTZ or placebo in high altitude

the heart rate

blood oxygen saturation and the incidence of acute altitude sickness were compared.

Results

The different doses of MTZ (400

200 and 100 mg/kg) significantly prolonged the closed hypoxia survival time than the control mice without treatment [25.01(2.40)

21.11(4.64) and 16.39(5.14)

14.62(3.62) min/100 mL

0.05

]

.Wha

t's more

200 mg/kg MTZ also improved myocardial hypoxia tolerance time (13.08±1.35

8.30±0.94 min/100 mL

0.01)and mouth movement time after decapitation of mice (43.22±8.23

20.44±2.60 s

0.01).In clinic trial

MTZ treatment significantly decreased HR[100(17)

90(21.5)

0.05

]

improve SaO

[85%(4%)

87%(3.5%)

0.01

]

and reduced the incidence rate of acute mountain sickness (19.4%

3.45%

0.05) in the volunteers at high altitude.

Conclusion

MTZ improves body's ability to resist hypoxia and effectively prevents the occurrence of acute high-altitude disease.

关键词

Keywords

references

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