Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor

Jiankui LI; Zhiqing LIANG; Huicheng XU

doi:10.16016/j.1000-5404.201707156

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Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor

Basic Medicine | 更新时间：2026-01-09

- Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor
- Journal of Third Military Medical University Vol. 39, Issue 22, Pages: 2176-2181(2017)
- 作者机构：
  
  400038 重庆，第三军医大学西南医院妇产科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81471443)
- DOI：10.16016/j.1000-5404.201707156
  CLC： R318.08;R322.62;R329.28
- Received：24 July 2017，
  
  Revised：2017-08-18，
  
  Published：30 November 2017
- 稿件说明：
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Jiankui LI, Zhiqing LIANG, Huicheng XU. Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor[J]. Journal of Third Military Medical University, 2017, 39(22): 2176-2181.
DOI：

Jiankui LI, Zhiqing LIANG, Huicheng XU. Evaluation of biocompatibility of porcine urinary bladder matrix loaded with both anti-sca-1 and basic fibroblast growth factor[J]. Journal of Third Military Medical University, 2017, 39(22): 2176-2181. DOI： 10.16016/j.1000-5404.201707156.

摘要

目的

观察负载抗sca-1抗体(anti-sca-1)和碱性成纤维细胞生长因子(basic fibroblast growth factor

bFGF)-脱细胞膀胱基质(urinary bladder matrix

UBM)生物支架的生物学性能，探讨其作为新型盆底修复材料的可能性。

方法

通过双特异性化学交联试剂4-(N-马来酰亚胺甲基)环己烷-1-羧酸磺酸基琥珀酰亚胺酯钠盐(sulfosuccinimidyl 4-[N-maleimidomethyl] cyclohexane-1-carboxylate

sulfo-smcc)将anti-sca-1和bFGF特异性结合到UBM上，得到anti-sca-1/bFGF-UBM生物支架。通过扫描电子显微镜观察生物支架表面结构，CCK-8(cell counting kit-8)间接检测体外富集sca-1阳性细胞情况，免疫荧光观察支架诱导小鼠骨髓间充质干细胞(mouse mesenchymal stem cells，mMSCs)分化成熟情况。

结果

扫描电子显微镜显示anti-sca-1和bFGF能特异性的结合到UBM上。anti-sca-1和bFGF组荧光强度明显高于对照组(

0.05)。anti-sca-1/bFGF-UBM生物支架富集的sca-1阳性细胞明显较对照组多(

0.05)。免疫荧光观察结果提示anti-sca-1/bFGF-UBM生物支架可以促进mMSCs向平滑肌细胞分化。

结论

anti-sca-1/bFGF-UBM生物支架显示出了良好的生物性能，既能富集sca-1阳性细胞，又能进一步促进细胞分化成熟。

Abstract

Objective

To evaluate the biocompatibility of porcine urinary bladder matrix (UBM) loaded with anti-sca-1 antibody and basic fibroblast growth factor (bFGF) and test its feasibility as a new material for repairing pelvic prolapse.

Methods

A bispecific cross-linking reagent sulfosuccinimidyl 4-[N-maleimidomethyl

]

cyclohexane-1-carboxylate (sulfo-smcc) was used to bind anti-sca-1 and bFGF to the UBM to obtain the anti-sca-1/bFGF-UBM biological scaffold. The structure of this scaffold material was observed using scanning electron microscopy

and its performance in enriching sca-1-positive cells

in vitro

was evaluated using cell counting kit-8 (CCK-8) assay. Immunofluorescence assay was employed to assess the differentiation of mouse mesenchymal stem cells (mMSCs) seeded in this scaffold material.

Results

Scanning electron microscopy showed that anti-sca-1 and bFGF could specifically bind to the UBM. The anti-sca-1/bFGF-UBM biological scaffold exhibited a significantly stronger fluorescence intensity than the control material (

0.05) and effectively enriched sca-1-positive cells

in vitro

(

0.05). Immunofluorescence assay confirmed that mMSCs seeded in this scaffold material were capable of differentiating into smooth muscle cells.

Conclusion

The anti-sca-1/bFGF-UBM scaffold shows a good biocompatibility.

关键词

Keywords

references

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