Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells

Wanxiang QIN; Min LUO; Ying SHI; Jian CUI; Fachuan NIE; Shifang LUO; Guiqiong HE; Kejian WANG

doi:10.16016/j.1000-5404.201706124

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Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells

Basic Medicine | 更新时间：2026-01-09

- Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells
- Journal of Third Military Medical University Vol. 39, Issue 22, Pages: 2189-2194(2017)
- 作者机构：
  
  1.400016 重庆，重庆医科大学神经科学研究中心
  2.400038 重庆，第三军医大学西南医院疼痛科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81371221);the Project of Basic and Frontier Research of Chongqing(CSTC2016jcyjA0069);the Project of Scientific and Technological Research of Chongqing Municipal Education Commission(KJ130317);the Science and Technology Project of Yuzhong District, Chongqing(20130132)
- DOI：10.16016/j.1000-5404.201706124
  CLC： R282.71;R322.8;R364.5
- Received：22 June 2017，
  
  Revised：2017-08-17，
  
  Published：30 November 2017
- 稿件说明：
移动端阅览
Wanxiang QIN, Min LUO, Ying SHI, et al. Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells[J]. Journal of Third Military Medical University, 2017, 39(22): 2189-2194.
DOI：

Wanxiang QIN, Min LUO, Ying SHI, et al. Inhibitory effects of dihydroartemisinin on LPS-induced inflammatory response in BV-2 microglial cells[J]. Journal of Third Military Medical University, 2017, 39(22): 2189-2194. DOI： 10.16016/j.1000-5404.201706124.

摘要

目的

观察双氢青蒿素(dihydroartemisinin，DHA)对脂多糖(lipopolysaccharide，LPS)诱导的BV-2小胶质细胞神经炎症反应的影响及其机制。

方法

LPS诱导BV-2小胶质细胞活化建立炎症模型，用不同浓度DHA(0.5、1、2、4 μmol/L)处理细胞，CCK-8检测细胞活性；选取1 μmol/L DHA干预细胞，倒置相差显微镜下观察细胞形态的变化；RT-PCR检测iNOS、IL-1β、IL-6、TNF-α基因表达水平；ELISA检测培养基中IL-1β、IL-6、TNF-α的表达水平；Western blot检测NF-κB、IκBα及TLR4的蛋白表达。

结果

低剂量的DHA(

2 μmol/L)对BV-2细胞活性无明显影响(

0.05)，DHA可抑制LPS引起的BV-2细胞形态变化，DHA抑制活化的BV-2细胞iNOS、IL-1β、IL-6、TNF-α mRNA的表达(

0.01)，减少IL-1β、IL-6、TNF-α炎症因子释放(

0.01)，明显降低TLR4的蛋白表达，减少细胞质内IκBα的表达，并抑制NF-κB向核内移位(

0.01)。

结论

DHA可能通过作用于TLR4/NF-κB通路，抑制LPS诱导的BV-2小胶质细胞NF-κB的激活，从而抑制炎症因子的产生，发挥抗炎作用。

Abstract

Objective

To investigate the inhibitory effect of dihydroartemisinin (DHA) on lipopolysaccharide (LPS)-induced inflammatory response in BV-2 microglial cells.

Methods

Inflammatory model was established by exposure of BV-2 cells to LPS for 24 h. Then after the cells were treated with different concentrations of DHA (0.5

2 and 4 μmol/L)

cell viability was tested with CCK-8 assay. The cell morphology was observed under inverted phase contrast microscope after the inflammatory cell model under the treatment of a harmless concentration (1 μmol/L) of DHA

The mRNA expression of iNOS

IL-1β

IL-6 and TNF-α was measured by RT-PCR. The contents of TNF-α

IL-1β and IL-6 in the supernatant were detected by ELISA. The expression of the nuclear NF-κB (p65)

IκBα

and TLR4 were analyzed by Western blotting.

Results

DHA showed no significant effect on cell viability when the concentration was lower than 2 μmol/L (

0.05). DHA inhibited the cell morphological changes induced by LPS. DHA treatment resulted in the decreases in the mRNA expression levels of iNOS

IL-1β

IL-6 and TNF-α (

0.01)

the production of pro-inflammatory cytokines IL-1β

IL-6

TNF-α

the protein levels of TLR4

intracytoplasmic IκBα and the nuclear translocation of NF-κB p65 (

0.01).

Conclusion

DHA inhibits the inflammatory responses

activation of NF-κB of BV-2 microglial cells induced by LPS

and thus suppresses the production of pro-inflammatory cytokines

via

TLR4/NF-κB signaling pathway.

关键词

Keywords

references

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