Long-chain non-coding ATB regulates invasion and metastasis of breast cancer cells by inhibiting miR-200c expression

Juan LI; Li HE; Qingshan GAO

doi:10.16016/j.1000-5404.201706106

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Long-chain non-coding ATB regulates invasion and metastasis of breast cancer cells by inhibiting miR-200c expression

Basic Medicine | 更新时间：2026-01-09

- Long-chain non-coding ATB regulates invasion and metastasis of breast cancer cells by inhibiting miR-200c expression
- Journal of Third Military Medical University Vol. 39, Issue 22, Pages: 2163-2168(2017)
- 作者机构：
  
  610000 成都，四川省人民医院城东病区乳腺甲状腺外科
- 作者简介：
- 基金信息：
  
  the Scientific Research Project of Health and Family Planning Commission of Sichuan Province(110137)
- DOI：10.16016/j.1000-5404.201706106
  CLC： R394.3; R73-37; R737.9
- Received：13 June 2017，
  
  Revised：2017-08-11，
  
  Published：30 November 2017
- 稿件说明：
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Juan LI, Li HE, Qingshan GAO. Long-chain non-coding ATB regulates invasion and metastasis of breast cancer cells by inhibiting miR-200c expression[J]. Journal of Third Military Medical University, 2017, 39(22): 2163-2168.
DOI：

Juan LI, Li HE, Qingshan GAO. Long-chain non-coding ATB regulates invasion and metastasis of breast cancer cells by inhibiting miR-200c expression[J]. Journal of Third Military Medical University, 2017, 39(22): 2163-2168. DOI： 10.16016/j.1000-5404.201706106.

摘要

目的

探讨长链非编码ATB通过抑制miR-200c的表达促进乳腺癌细胞侵袭转移的作用及其机制。

方法

qPCR检测不同乳腺癌细胞株中ATB和miR-200c的表达情况；双荧光素酶报告基因检测ATB与miR-200c的相互作用；MTT细胞增殖实验和Transwell侵袭实验检测沉抑制ATB后乳腺癌细胞增殖和侵袭能力的变化；MTT细胞增殖实验和Transwell侵袭实验检测抑制ATB后沉默miR-200c对乳腺癌细胞增殖和侵袭能力的逆转作用；Western blot检测抑制ATB后ZEB1和ZNF217蛋白的表达。

结果

与其他乳腺癌细胞相比，SKBr-3细胞株ATB表达水平最低，miR-200c的表达水平最高；ATB能与miR-200c的位点特异性结合，调控其表达活性；抑制ATB后可以增强乳腺癌细胞的增殖和侵袭能力，而沉默miR-200c后可以在一定程度上逆转ATB对乳腺癌细胞增殖和侵袭能力的影响；抑制ATB后ZEB1和ZNF217蛋白表达水平得到一定的恢复。

结论

ATB在乳腺癌发生、发展过程中起重要作用，ATB可以靶向调节miR-200c调控乳腺癌细胞的增殖和侵袭能力。

Abstract

Objective

To determine the effect of long-chain non-coding ATB on the invasion and metastasis of breast cancer cells

and investigate its underlying mechanism.

Methods

The expression levels of ATB and miR-200c in different breast cancer cell lines (BT474

SKBr-3

MCF7 and MDA-MB-453) were detected by qPCR. Double luciferase reporter gene was used to detect the interaction between the 2 molecules. MTT assay and Transwell invasion assay were used to detect the proliferation and invasion of breast cancer cells after ATB inhibition. The reversing effect of miR-200c on the proliferation and invasion of breast cancer cells after ATB inhibition was also detected. Western blotting was used to detect the expression of ZEB1 and ZNF217 protein after ATB inhibition.

Results

Compared with other breast cancer cells

SKBr-3 cells had lowest ATB and highest miR-200c expression levels. ATB could bind specifically to the site of miR-200c and regulate its expression activity. Inhibition of ATB enhanced the proliferation and invasion of breast cancer cells

but silencing miR-200c could reverse the effects of ATB inhibition to a certain extent. After ATB inhibition

the protein expression levels of ZEB1 and ZNF217 were restored partially.

Conclusion

ATB plays an important role in the incidence and development of breast cancer. ATB targets miR-200c and then regulates the proliferation and invasion of breast cancer cells.

关键词

Keywords

references

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