Drug resistance profiles and metallo-β-lactamase and outer membrane protein OprD2 genotypes of <italic style="font-style: italic">Pseudomonas aeruginosa</italic>: a 2014-2016 survey in a Grade 3 Class A general hospital in Chongqing

Jin LI; Weiwei HU; Fengling ZHANG; Ming CHEN; Shaoli DENG; Weiping LU

doi:10.16016/j.1000-5404.201706013

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Drug resistance profiles and metallo-β-lactamase and outer membrane protein OprD2 genotypes of Pseudomonas aeruginosa: a 2014-2016 survey in a Grade 3 Class A general hospital in Chongqing

Clinical Medicine | 更新时间：2026-01-09

- Drug resistance profiles and metallo-β-lactamase and outer membrane protein OprD2 genotypes of Pseudomonas aeruginosa: a 2014-2016 survey in a Grade 3 Class A general hospital in Chongqing
- Journal of Third Military Medical University Vol. 39, Issue 22, Pages: 2200-2205(2017)
- 作者机构：
  
  1.400042 重庆，第三军医大学大坪医院野战外科研究所检验科
  2.400014 重庆，重庆医科大学附属儿童医院检验科
- 作者简介：
- 基金信息：
  
  the General Program of the "Twelfth Five-Year Plan" for Sci & Tech Research of PLA(CWS13J039);the Key Project of National Natural Science Foundation of China(81430053)
- DOI：10.16016/j.1000-5404.201706013
  CLC： R181.34;R378.991;R915
- Received：03 June 2017，
  
  Revised：2017-09-19，
  
  Published：30 November 2017
- 稿件说明：
移动端阅览
Jin LI, Weiwei HU, Fengling ZHANG, et al. Drug resistance profiles and metallo-β-lactamase and outer membrane protein OprD2 genotypes of Pseudomonas aeruginosa: a 2014-2016 survey in a Grade 3 Class A general hospital in Chongqing[J]. Journal of Third Military Medical University, 2017, 39(22): 2200-2205.
DOI：

Jin LI, Weiwei HU, Fengling ZHANG, et al. Drug resistance profiles and metallo-β-lactamase and outer membrane protein OprD2 genotypes of Pseudomonas aeruginosa: a 2014-2016 survey in a Grade 3 Class A general hospital in Chongqing[J]. Journal of Third Military Medical University, 2017, 39(22): 2200-2205. DOI： 10.16016/j.1000-5404.201706013.

摘要

目的

检测亚胺培南耐药铜绿假单胞菌金属酶基因分布和外膜孔蛋白OprD2基因缺失情况，了解铜绿假单胞菌的耐药状况。

方法

收集第三军医大学大坪医院野战外科研究所2014-2016年临床分离的非重复铜绿假单胞菌1 887株，VITEK-MS质谱仪进行菌株鉴定，最小抑菌浓度(minimal inhibitory concentration，MIC)法测定其对10种常用抗菌药物的敏感性，利用WHONET5.6软件对药敏资料进行统计分析，同时采用亚胺培南-EDTA纸片法筛选金属酶表型阳性的菌株，用聚合酶链反应(polymerase chain reaction，PCR)测铜绿假单胞菌金属β-内酰酶(metallo-β-lactamase，MBL)相关基因(IMP、VIM、SPM、GIM、SIM、NDM)和外膜孔蛋白OprD2基因的情况。

结果

3年中铜绿假单胞菌每年的分离数量分别为756株(9.4%)、579株(9.5%)和552株(10.7%)，主要来自ICU病房和呼吸内科病房，标本类型以痰液为主；2014-2016年分离出的铜绿假单胞菌对妥布霉素、庆大霉素的耐药率逐年下降，其耐药率分别从2014年的11.3%、13.2%下降至2016年的7.8%、9.0%，对左旋氧氟沙星、哌拉西林/他唑巴坦的耐药率呈上升趋势，其耐药率从2014年的10.7%、9.2%上升至2016年的23.5%、11.6%。从基因型分布情况来看，269株亚胺培南耐药铜绿假单胞菌中MBL基因阳性51株(18.9%)，外膜孔蛋白OprD2缺失158株(58.7%)；51株产酶菌株中VIM型阳性29株，SPM型阳性22株，未检测出其他基因型。

结论

铜绿假单胞菌对亚胺培南的耐药率较高；产MBL和外膜孔蛋白OprD2基因缺失是引起铜绿假单胞菌对亚胺培南耐药的主要原因。

Abstract

Objective

To investigate the drug resistance profiles of

Pseudomonas aeruginosa

isolates and survey the distribution of metallo-β-lactamase (MBL) phenotypes and outer membrane protein OprD2 gene deletion among the strains.

Methods

From January 2014 to December 2016

a total of 1 887 non-duplicate clinical isolates of

Pseudomonas aeruginosa

were collected from Daping Hospital. All the strains were identified by VITEK-MS

and their susceptibilities to 10 antimicrobial agents were detected using the minimal inhibitory concentration method. WHONET5.6 software was used to analyze the results of drug sensitivity tests. Imipenem-EDTA disc test was used to detect the MBL phenotypes of the strains

and the MBL genes (IMP

VIM

SPM

GIM

SIM

and NDM) and OprD2 gene were detected by PCR amplification.

Results

Among the total of 1 887

Pseudomonas aeruginosa

strains isolated

756 (9.4%) were isolated in 2014

579 (9.5%) in 2015

and 552 (10.7%) in 2016; most of the strains were isolated from the sputum samples in the Department of Respiratory Medicine and ICU. From January 2014 to December 2016

the drug resistance rates of the isolated strains to tobramycin and gentamicin decreased progressively (from 11.3% and 13.2% in 2014 to 7.8% and

9.0% in 2016

respectively)

while their resistance rates to levofloxacin and piperacillin/tazobactam tended to increase (from 10.7% and 9.2% in 2014 to 23.5% and 11.6% in 2016

respectively). Of the 269 imipenem-resistant isolates

51 (18.9%) were MBL-producing strains (including 29 VIM type MBL-positive and 22 SPM type MBL-positive isolates)

and 158 (58.7%) showed deletion of OprD2 gene; no other genotypes were detected among the isolated strains.

Conclusion

We found a high incidence of drug resistance (particularly imipenem) in the clinical isolates of

Pseudomonas aeruginosa

. MBL production and the loss of OprD2 gene are the main contributors to imipenem resistance in

Pseudomonas aeruginosa

关键词

Keywords

references

陈媛媛. 铜绿假单胞菌的金属β-内酰胺酶基因型研究[D]. 长春: 吉林大学, 2012.

CHEN Y Y. Study on the genotypes of metallo-β-lactamases in pseudomonas aeruginosa[D]. Changchun: Jilin University, 2012.

邱春华, 王静, 刘玉敏. 330株铜绿假单胞菌耐药情况分析[J].第三军医大学学报, 2014, 36(15): 1613, 1618.

QIU C H, WANG J, LIU YM. Analysis of drug resistance of 330 strains of Pseudomonas aeruginosa [J ] . J Third Mil Med Univ, 2014, 36(15): 1613, 1618.

RODRíGUEZ-MARTíNEz J M, POIREl L, NORDMANN P. Molecular epidemiology and mechanisms of carbapenem resistance in Pseudomonas aeruginosa [J ] . Antimicrob Agents Chemother, 2009, 53(11): 4783-4788. DOI: 10.1128/AAC.00574-09.

TATO M, COQUE T M, BAQUERO F, et al . Dispersal of carbapenemase blaVIM-1 gene associated with different Tn402 variants, mercury transposons, and conjugative plasmids in Enterobacteriaceae and Pseudomonas aeruginosa [J ] . Antimicrob Agents Chemother, 2010, 54(1): 320-327. DOI: 10.1128/AAC.00783-09..

HENTSCHKE M, GORITZKA V, CAMPOS C B, et al . Emergence of carbapenemases in Gram-negative bacteria in Hamburg, Germany[J ] . Diagn Microbiol Infect Dis, 2011, 71(3): 312-315. DOI: 10.1016/j.diagmicrobio. 2011.07.011.

VATCHEVA-DOBREVSKA R, MULET X, IVANOV I, et al . Molecular epidemiology and multidrug resistance mechanisms of Pseudomonas aeruginosa isolates from Bulgarian hospitals[J ] . Microb Drug Resist, 2013, 19(5): 355-361. DOI: 10.1089/mdr.2013.0004..

黄义山, 龙彦, 方莉, 等.多药耐药铜绿假单胞菌金属β-内酰胺酶及相关基因检测[J].中华临床医师杂志(电子版), 2010, 4(9): 120-123.

HUANG Y S, LONG Y, FANG L, et al . Study on the resistant genes of metal β-lactamase(MBLs) of the multi-drug resistant mechanism of Pseudomonas aeruginosa [J ] . Chin J Clinicians (Electronic Edition), 2010, 4(9): 120-123..

应春妹, 应骏, 罗柳林, 等.耐亚胺培南铜绿假单胞菌耐药机制研究[J].中国感染与化疗杂志, 2008, 8(4): 300-302.

YING C M, YING J, LUO L L, et al . Mechanism of imipenem resistance in Pseudomonas aeruginosa[J ] . Chin J Infect Chemother, 2008, 8(4): 300-302.

张春平, 喻华, 刘华, 等.铜绿假单胞菌感染分布及耐药性动态变迁[J].中华医院感染学杂志, 2008, 18(1): 121-123.

ZHANG C P, YU H, LIU H, et al . Dynamic changes of drug resistance and infectious distribution of Pseudomonas aeruginosa [J ] . Chin J Nosocomiol, 2008, 18(1): 121-123..

杨政. 2009-2011年重庆某三甲医院铜绿假单胞菌耐药性分析[D]. 重庆: 重庆医科大学, 2013.

YANG Z. Resistance surveillance of Pseudomonas aeruginosa in a chongqing grade AAA hospital from 2009 to 2011 [D ] . Chongqing: Chongqing Medical University, 2013.

张祎博, 孙景勇, 倪语星, 等. 2005~2014年CHINET铜绿假单胞菌耐药性监测[J].中国感染与化疗杂志, 2016, 16(2): 141-145.

ZHANG Y B, SUN J Y, NI Y X, et al . Resistance profile of Pseudomonas aeruginosa in hospitals across China: the results from the CHINET Antimicrobial Resistance Surveillance Program, 2005-2014[J ] . Chin J Infect Chemother, 2016, 16(2):141-145..

郭小兵, 贺小红, 曹在秋, 等.临床分离铜绿假单胞菌10662株的分布及耐药性变迁[J].中国感染与化疗杂志, 2016, 16(5): 627-630. DOI: 10.16718/j.1009-7708.2016.05.018.

GUO X B, HE X H, CAO Z Q, et al . Changing distribution and antibiotic resistance profile in 10 662 clinical strains of Pseudomonas aeruginosa [J ] . Chin J Infect Chemother, 2016, 16(5): 627-630. DOI: 10.16718/j.1009-7708. 2016.05.018..

VALENZA G, JOSEPH B, ELIAS J, et al . First survey of metallo-beta-lactamases in clinical isolates of Pseudomonas aeruginosa in a German university hospital[J ] . Antimicrob Agents Chemother, 2010, 54(8): 3493-3497. DOI: 10.1128/AAC.00080-10..

JOVCIC B, LEPSANOVIC Z, SULJAGIC V, et al . Emergence of NDM-1 metallo-β-lactamase in Pseudomonas aeruginosa clinical isolates from Serbia[J ] . Antimicrob Agents Chemother, 2011, 55(8): 3929-3931. DOI: 10.1128/AAC.00226-11..

卓超, 伍晓锋, 金光耀, 等.广州地区泛耐药铜绿假单胞菌的分子流行病学调查[J].中华检验医学杂志, 2008, 31(6): 643-647. DOI: 10.3321/j.issn:1009-9158.2008. 06.008.

ZHUO C, WU X F, JIN G Y, et al . Investigation on epidemiology of pan-drug resistant Pseudomonas aeruginosa in Guangzhou [J ] . Chin J Lab Med, 2008, 31(6): 643-647. DOI: 10.3321/j.issn:1009-9158.2008.06.008..

周志慧, 吕芳芳, 周建英, 等.中国部分地区铜绿假单胞菌耐药性及主要β内酰胺酶基因型分布[J].中华传染病杂志, 2010, 28(10): 577-581. DOI:10.3760/cma.j.issn.1000-6680.2010.10.001.

ZHOU Z H, LV F F, ZHOU J Y, et al . Resistance of Pseudomonas aeruginosa and genotyping of the main β-lactamases in China [J ] . Chin J Infect Dis, 2010, 28(10): 577-581. DOI:10.3760/cma.j.issn.1000-6680.2010.10.001..

邬靖敏, 邹明祥, 李军.长沙地区产金属β-内酰胺酶铜绿假单胞菌的分子流行病学特征[J].中国感染控制杂志, 2016, 15(7): 447-451. DOI: 10.3969/j.issn.1671-9638. 2016.07.002.

WU J M, ZOU M X, LI J. Molecular epidemiological characteristics of metallo-β-lactamase-producing Pseudomonas aeruginosa from Changsha [J ] . Chin J Infect Control, 2016, 15(7): 447-451. DOI: 10.3969/j.issn.1671-9638.2016.07.002.

胥飚, 王东升, 余显书.耐亚胺培南铜绿假单胞菌的耐药性及相关基因研究[J].重庆医科大学学报, 2009, 34(4): 466-468.

XU B, WANG D S, YU X S. A study of drug resistance and related genes of imipenem-resistant Pseudomonas aeruginosa [J ] . J Chongqing Med Univ, 2009, 34(4): 466-468.

程新. 铜绿假单胞菌耐药分析与耐亚胺培南菌株基因型检测研究[D]. 贵阳: 贵州医科大学, 2016.

CHENG X. Investigation of drug resistance and genes in imipenem-resistant Pseudomonas aeruginosa [D ] . Guiyang: Guizhou Medical University, 2016.

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⁰

Drug resistance profiles and metallo-β-lactamase and outer membrane protein OprD2 genotypes of Pseudomonas aeruginosa: a 2014-2016 survey in a Grade 3 Class A general hospital in Chongqing

DOI：10.16016/j.1000-5404.201706013

摘要

Abstract

关键词

Keywords

references