Resveratrol improves autistic behaviors in rat model

Xufang DAI; Liyan QIN; Jiqin LIAN

doi:10.16016/j.1000-5404.201703153

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Resveratrol improves autistic behaviors in rat model

Basic Medicine | 更新时间：2026-01-09

- Resveratrol improves autistic behaviors in rat model
- Journal of Third Military Medical University Vol. 39, Issue 13, Pages: 1360-1365(2017)
- 作者机构：
  
  1.400047 重庆，重庆师范大学教育科学学院, 重庆市特殊儿童心理诊断与教育技术重点实验室
  2.400047 重庆，重庆师范大学教育科学学院, 特殊教育系
  3.400038 重庆，第三军医大学, 西南医院输血科
  4.400038 重庆，第三军医大学, 基础医学部生物化学与分子生物学教研室
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(31201068)
- DOI：10.16016/j.1000-5404.201703153
  CLC： R-332;R285.6;R749.94
- Received：24 March 2017，
  
  Revised：2017-04-16，
  
  Published：15 July 2017
- 稿件说明：
移动端阅览
Xufang DAI, Liyan QIN, Jiqin LIAN. Resveratrol improves autistic behaviors in rat model[J]. Journal of Third Military Medical University, 2017, 39(13): 1360-1365.
DOI：

Xufang DAI, Liyan QIN, Jiqin LIAN. Resveratrol improves autistic behaviors in rat model[J]. Journal of Third Military Medical University, 2017, 39(13): 1360-1365. DOI： 10.16016/j.1000-5404.201703153.

摘要

目的

探讨白藜芦醇(resveratrol)改善孤独症大鼠病症行为的作用及可能机制。

方法

于大鼠孕12.5 d丙戊酸钠(sodium valproate，VPA)腹腔一次性注射制备孤独症幼鼠模型。白藜芦醇处理组从母鼠怀孕后第6天开始按照3.6 mg/kg体质量在孕鼠皮下注射白藜芦醇，持续注射13 d。将幼鼠分为4组：对照组、白藜芦醇处理组、VPA处理组、VPA联合白藜芦醇处理组，每组3只。出生后35 d对幼鼠进行重复行为、神经行为与社会交往行为检测，通过Western blot检测脑组织中自噬标志蛋白LC3及SIRT1表达变化。

结果

成功获得孤独症模型大鼠。与对照组相比，VPA组重复行为增强、社会交往能力下降、中央区活动时间与距离增加、站立次数减少(

0.05)，符合孤独症行为特征；白藜芦醇组无明显行为学变化；但白藜芦醇联合处理可显著改善VPA处理引起的孤独症行为症状(

0.05)，包括重复行为减弱，社会交往能力增强，中央区活动时间与距离减少、站立次数增加。Western blot检测结果显示，与对照组相比，VPA处理可降低大鼠脑组织中SIRT1表达，并抑制LC3-Ⅱ表达水平；而白藜芦醇联合处理则可增强大脑组织中SIRT1与LC3-Ⅱ的表达。

结论

白藜芦醇能改善孤独症模型大鼠的病症行为，机制可能与增强细胞自噬及SIRT1表达相关。

Abstract

Objective

To determine the effect of resveratrol on the behaviors of autistic rats and investigate the possible mechanism.

Methods

Rat model of autism was inflicted by intraperitoneal injection of sodium valproate (VPA) in rats after pregnancy for 12.5 d. The pregnant rats of the resveratrol group were treated with resveratrol

via

subcutaneous injection at a dose of 3.6 mg/kg per day from E6 to E18. Then the offspring were randomly divided to 4 groups: control

VPA treatment

resveratrol treatment

and VPA+resveratrol treatment groups (

=3 for each group). The repetitive behavior

neural behavior and social interaction were evaluated in the young rats in 35 d after born. The expression levels of LC3-Ⅱ and SIRT1 in brain tissues were detected by Western blotting.

Results

The rat model of autism was established successfully. Compared to the control rats

the rats treated with VPA showed excessive repetitive behavior

lower social interaction

longer moving time and distance in central area

and reduced standing times (

0.05)

which were in accordance with the typical behaviors of autistic rats. Treatment with resveratrol alone resulted in no obvious changes in the social interaction and neural behavior in the young rats

but resveratrol treatment corrected the VPA-induced autistic-like behaviors (

0.05). Western blotting indicated that VPA treatment reduced the levels of SIRT1and LC3-Ⅱin the brain tissues including prefrontal lobe

seahorse and cerebellum. However

resveratrol attenuated VPA-triggered down-regulation of SIRT1 and LC3-Ⅱ.

Conclusion

Resveratrol may correct the behaviors of autistic rats through SIRT1 enhancement mediated autophagy up-regulation.

关键词

Keywords

references

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