IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells <italic style="font-style: italic">in vitro</italic>

Zhifen LI; Junjie SHEN; Juanjuan SHAN; Jun CHEN; Cheng QIAN

doi:10.16016/j.1000-5404.201703093

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IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro

更新时间：2026-01-09

- IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro
- Journal of Third Military Medical University Vol. 39, Issue 16, Pages: 1631-1636(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81472292)
- DOI：10.16016/j.1000-5404.201703093
  CLC：
- Received：15 March 2016，
  
  Published：30 August 2017
- 稿件说明：
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Zhifen LI, Junjie SHEN, Juanjuan SHAN, et al. IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro[J]. Journal of Third Military Medical University, 2017, 39(16): 1631-1636.
DOI：

Zhifen LI, Junjie SHEN, Juanjuan SHAN, et al. IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro[J]. Journal of Third Military Medical University, 2017, 39(16): 1631-1636. DOI： 10.16016/j.1000-5404.201703093.

摘要

Abstract

Objective

To investigate the role of FOXO3a in the regulation of self-renewal capacity in liver cancer stem cells (LCSCs) and study the related mechanisms.

Methods

Flow cytometry was used to obtain LCSCs

and then the expression of FOXO3a in the attained cells was detected by real time fluorescent quantitative PCR (qRT-PCR). After the activity of insulin-like growth factor(IGF) was changed with IGF inhibitor or activator

the expression of FOXO3a was measured again with Western blotting and qRT-PCR. The lentiviral vectors expressing or interfering FOXO3a were used to infect the cells

and Western blot analysis was used to detect the efficiencies of overexpression and interference. Cell colony formation assay and sphere formation assay were used to test the self-renewal in the cells infected with the lentivirus that expressing or interfering FOXO3a

and the cells treated with IGF activators meanwhile over-expressing FOXO3a.

Results

The expression of FOXO3a was significantly lower in the LCSCs than the liver cancer cells (

0.01)

and it was negatively regulated by IGF. The cell models of stable overexpression or interference of FOXO3a were established successfully. And FOXO3a over-expression resulted in decreases in the colony formation and sphere formation

while FOXO3a interference led to improvement in the formations. Activation of IGF signal

combined with FOXO3a overexpression also induced decreased formations.

Conclusion

FOXO3a exerts a negative effect on the self-renewal in LCSCs

and can be negatively regulated by IGF signaling. It mediates the regulation of IGF signaling on the self-renewal in LCSCs.

关键词

Keywords

references

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IGF-FOXO3a pathway regulates self-renewal of liver cancer stem cells in vitro

DOI：10.16016/j.1000-5404.201703093

摘要

Abstract

关键词

Keywords

references