Characteristics of different kinds of bacillus by Terahertz time-domain spectroscopy

Wenjing YU; Xiang YANG; Yu LIU; Xiang ZHAO; Zhongquan LIN; Ke YANG; Weiling FU

doi:10.16016/j.1000-5404.201703025

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Characteristics of different kinds of bacillus by Terahertz time-domain spectroscopy

Basic Medicine | 更新时间：2026-01-09

- Characteristics of different kinds of bacillus by Terahertz time-domain spectroscopy
- Journal of Third Military Medical University Vol. 39, Issue 13, Pages: 1315-1320(2017)
- 作者机构：
  
  1.400038 重庆，第三军医大学西南医院检验科
  2.400023 重庆，中国人民解放军第三二四医院检验科
- 作者简介：
- 基金信息：
  
  the National Basic Research Program of China(2015CB755400);the National Basic Research and Development Program(2015CB755402);the Key Program of National Natural Science Foundation of China(81430054);the Key Project of Military Technical Product Research(BWS13C013)
- DOI：10.16016/j.1000-5404.201703025
  CLC： R312;R378;R446
- Received：04 March 2017，
  
  Revised：2017-03-30，
  
  Published：15 July 2017
- 稿件说明：
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Wenjing YU, Xiang YANG, Yu LIU, et al. Characteristics of different kinds of bacillus by Terahertz time-domain spectroscopy[J]. Journal of Third Military Medical University, 2017, 39(13): 1315-1320.
DOI：

Wenjing YU, Xiang YANG, Yu LIU, et al. Characteristics of different kinds of bacillus by Terahertz time-domain spectroscopy[J]. Journal of Third Military Medical University, 2017, 39(13): 1315-1320. DOI： 10.16016/j.1000-5404.201703025.

摘要

目的

应用太赫兹时域光谱技术对常见致病芽孢杆菌进行检测。

方法

使用太赫兹时域光谱技术对枯草芽孢杆菌、蜡状芽孢杆菌、苏云金芽孢杆菌3种致病菌进行了室温、干燥环境下太赫兹时域光谱测试。得到了样品在0.2~2.0 THz波段的太赫兹吸收曲线。

结果

随着频率增加不同含量的芽孢杆菌冻干粉的太赫兹波段的吸收系数是增加的，并且有不同的吸收趋势；此外不同种属的芽孢杆菌的冻干粉和菌落也具有明显不同的吸收系数，这意味着3种样品的分子及官能团的构成和存在状态是不同的，更有力地揭示3种样品的确具有不同的生物成分组成和结构。

结论

太赫兹时域光谱技术作为一种快速高效、无需标记且无破坏性的探测方法，为芽孢杆菌的检测和鉴别提供了一种行之有效的方法。

Abstract

Objective

To detect the common disease-causing bacillus species by Terahertz time-domain spectroscopic (THz-TDS) technique.

Methods

Three kinds of bacillus species

Bacillus cereus

Bacillus subtilis

and

Bacillus thuringiensis

were tested and investigated with the THz-TDS technique at room temperature in dry environment.The absorption coefficient of 3 bacillus samples were calculated in the frequency of 0.2~2.0 THz.

Results

The absorption coefficients of lyophilized powder containing different amounts of bacillus species were increased as the increase of frequency

and had considerable different absorption trend among the samples.Different coefficients were also observed in the lyophilized powders of different bacillus species and bacterial colonies

which suggested the 3 kinds of species were made up of different molecular vibration and multi-molecular groups in the THz spectrum

and had different biological components and structure in the 3 bacillus samples.

Conclusion

THz-TDS is a fast

efficient

non-marking and non-destructive detection method

and provides an effective method for the detection and identification of bacillus species.

关键词

Keywords

references

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