Low-dose zearalenone exposure impairs reproductive capacity of male mice

Jian Pang; Zhongyi Sun

doi:10.16016/j.1000-5404.201702099

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Low-dose zearalenone exposure impairs reproductive capacity of male mice

Basic Medicine | 更新时间：2026-01-09

- Low-dose zearalenone exposure impairs reproductive capacity of male mice
- Journal of Third Military Medical University Vol. 39, Issue 12, Pages: 1225-1230(2017)
- 作者机构：
  
  400042 重庆，第三军医大学大坪医院野战外科研究所泌尿外科
- 作者简介：
- 基金信息：
  
  by the General Program of National Natural Science Foundation of China(31572225)
- DOI：10.16016/j.1000-5404.201702099
  CLC： R321.1;R977.12;R996.2
- Received：21 February 2017，
  
  Revised：2017-03-15，
  
  Published：30 June 2017
- 稿件说明：
移动端阅览
Jian Pang, Zhongyi Sun. Low-dose zearalenone exposure impairs reproductive capacity of male mice[J]. Journal of Third Military Medical University, 2017, 39(12): 1225-1230.
DOI：

Jian Pang, Zhongyi Sun. Low-dose zearalenone exposure impairs reproductive capacity of male mice[J]. Journal of Third Military Medical University, 2017, 39(12): 1225-1230. DOI： 10.16016/j.1000-5404.201702099.

摘要

目的

显作用水平的浓度)玉米赤霉烯酮(zearalenone，ZEA)暴露后小鼠精子发生及精液质量的变化趋势，探讨小剂量ZEA暴露对雄性小鼠生殖能力的影响。

方法

90只出生后21 d的雄性CD-1小鼠按随机数字表法分成3组，分别暴露于0、20、40 μg/(kg·d) ZEA，处理14、28、42 d后，采用计算机辅助精液质量分析观察精子活力参数，吉姆萨染色观测精子畸形率，测量睾丸体积及质量，免疫组化染色检测生精细胞计数。

结果

处理42 d后，20、40 μg/(kg·d)组精子活动率低于0 μg/(kg·d)组[(82.80±4.34)%、(74.90±6.70)%

(93.20±1.66)%，

=0.004、0.002

]

；20、40 μg/(kg·d)组小鼠畸形精子率高于0 μg/(kg·d)组[(29.40±1.09)%、(40.80±6.17)%

(19.40± 0.45)%，

0.001，

=0.002

]

；20、40 μg/(kg·d)组小鼠的单位面积生精细胞计数低于0 μg/(kg·d)组(2 422.7± 206.1、1 855.8±105.3

3 189.8±306.0，

=0.013、0.001)。

结论

小剂量ZEA的暴露能够降低雄性小鼠的精子发生及精液质量，损害其生殖能力。

Abstract

Objective

To investigate the changes of spermatogenesis and semen quality in mice exposed to low-dose (below the no-observed effect level) zearalenone (ZEA) and assess the effect of such exposures on the reproductive capacity of male mice.

Methods

Ninety male CD-1 mice (21 days old) were randomized into 3 groups for exposure to intragastrically administered ZEA at 0

20 and 40 μg/(kg·d) for 14

28 or 42 d. After the exposures

the sperm motility of the mice was assessed using the computer-aided semen analysis system

and sperm deformity was detected with Giemsa staining. The weight and volume of the testis were measured

and the count of spermatogenic cells per unit area was evaluated with immunohistochemical staining.

Results

Exposure to ZEA for 42dcaused significantly lowered sperm motility in 20 and 40 μg/(kg·d) dose groups in comparison with the unexposed group [(82.80±4.34)%

(74.90±6.70)%

and (93.20±1.66)%

respectively;

=0.004

0.002

]

. Compared with the unexposed group

exposure to 20 and 40 μg/(kg·d) ZEA resulted in significantly increased sperm deformity rate [(29.40±1.09)%

(40.80±6.17)%

(19.40±0.45)%

0.001

=0.002

]

and reduced counts of spermatogenic cells per unit area (2 422.7±206.1

1 855.8±1

05.3

3 189.8±306.0

=0.013

0.001).

Conclusion

Low-dose ZEA exposure impairs spermatogenesis and semen quality and damages the reproductive capacity in male mice.

关键词

Keywords

references

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