Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults

Jianfang Gao; Huan Yang; Peng Zou; Qing Chen; Guowei Zhang; Xi Ling; Hongqiang Chen; Lin Ao; Jia Cao

doi:10.16016/j.1000-5404.201702074

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Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults

Public Health and Preventive Medicine | 更新时间：2026-01-09

- Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults
- Journal of Third Military Medical University Vol. 39, Issue 12, Pages: 1286-1291(2017)
- 作者机构：
  
  1.750004 银川，宁夏医科大学公共卫生与管理学院
  2.400038 重庆，第三军医大学军事预防医学院毒理学研究所
- 作者简介：
- 基金信息：
  
  National Natural Science Foundation for Young Scholars of China(81202276)
- DOI：10.16016/j.1000-5404.201702074
  CLC： R321.1;R339.2;R394.2
- Received：15 February 2017，
  
  Revised：2017-03-08，
  
  Published：30 June 2017
- 稿件说明：
移动端阅览
Jianfang Gao, Huan Yang, Peng Zou, et al. Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults[J]. Journal of Third Military Medical University, 2017, 39(12): 1286-1291.
DOI：

Jianfang Gao, Huan Yang, Peng Zou, et al. Correlation of sperm mitochondrial DNA copy number and integrity with semen parameters in male adults[J]. Journal of Third Military Medical University, 2017, 39(12): 1286-1291. DOI： 10.16016/j.1000-5404.201702074.

摘要

目的

分析成年男性精子线粒体DNA(mitochondrial DNA，mtDNA)拷贝数及完整性与常规精液质量参数的关联，探讨精子mtDNA相关指标在反映男性生育力中的作用。

方法

采集161例参加男性生殖健康调查的志愿者的精液样本。采用计算机辅助精子分析系统(computer-aided sperm analysis，CASA)进行精液参数分析，Diff-Quick试剂盒染色检测精子形态。实时荧光定量PCR测定精子mtDNA拷贝数，长链PCR检测mtDNA完整性。

结果

mtDNA拷贝数与常规精液参数包括精液体积、精子密度、精子总数、前向运动率、精子活率和精子正常形态率均呈负相关(

=-0.168、-0.452、-0.474、-0.189、-0.306、-0.235，

均＜0.05)。除精子前向运动率外，mtDNA完整性与其余常规精液参数均呈正相关(

=0.193、0.379、0.480、0.325、0.282，

均＜0.05)。采用多元线性回归分析调整混杂因素后，mtDNA相关指标与精子密度、精子总数、精子活率和正常形态率之间的相关性仍然存在。

结论

mtDNA拷贝数和mtDNA完整性与常规精液质量参数之间存在关联，mtDNA相关指标可能是反映精子质量的生物标志物。

Abstract

Objective

To analyze the association of sperm mitochondrial DNA (mtDNA) copy number and mtDNA integrity with semen quality parameters and investigate the role of the sperm mtDNA-related indicators in assessing fertility in male adults.

Methods

We collected semen samples from 161 male adult volunteers in a male reproductive health survey. The routine semen parameters were examined using a computer-aided sperm analysis system (CASA)

and Diff-Quick kit was used to evaluate the sperm morphology. Quantitative long polymerase chain reaction (L-PCR) and real-time fluorescence quantitative PCR (Q-PCR) were used to assess the mtDNA integrity and detect mtDNA copy number in the spermatozoa.

Results

We found inverse correlations of sperm mtDNA copy number with the semen volume

sperm concentration

total sperm count

progressive motile sperm count

total motility and the rate of normal sperm morphology (

=-0.168

-0.452

-0.474

-0.189

-0.306

-0.235

all

0.05). Sperm mtDNA integrity was positively correlated with the semen volume

sperm concentration

total sperm count

total motility and the rate of normal sperm morphology (

=0.193

0.379

0.480

0.325

0.282

all

0.05) except for progressive motile sperm count. Multiple linear regression analysis showed significant correlations of mtDNA copy number and mtDNA integrity with the sperm concentration

total sperm count

sperm motility

and the rate of normal sperm morphology after adjustment for the confounders.

Conclusion

Sperm mtDNA copy number and mtDNA integrity are significantly correlated with the routine semen quality parameters

suggesting that these two mtDNA-related indicators may serve as potential biomarkers for assessing sperm quality in male adults.

关键词

Keywords

references

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