Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography

Yan ZHOU; Jun FAN; Yehua JIANG; Jue ZHANG; Yi ZHANG; Lixin FU; Biao HUANG

doi:10.16016/j.1000-5404.201702048

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Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography

Basic Medicine | 更新时间：2026-01-09

- Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography
- Journal of Third Military Medical University Vol. 39, Issue 18, Pages: 1803-1807(2017)
- 作者机构：
  
  1.214063 江苏无锡，江苏省原子医学研究所
  2.214063 江苏无锡，无锡市江原实业技贸总公司
- 作者简介：
- 基金信息：
  
  the Project of Key Technology R & D Program for Social Development of Jiangsu Province(BE2014608);the Project of Preventive Medicine of Jiangsu Provincial Health and Family Planning Commission(Y2015072)
- DOI：10.16016/j.1000-5404.201702048
  CLC： R345.69;R446.112; R446.6
- Received：13 February 2017，
  
  Revised：2017-05-10，
  
  Published：30 September 2017
- 稿件说明：
移动端阅览
Yan ZHOU, Jun FAN, Yehua JIANG, et al. Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography[J]. Journal of Third Military Medical University, 2017, 39(18): 1803-1807.
DOI：

Yan ZHOU, Jun FAN, Yehua JIANG, et al. Quantitative detection of serum pepsinogen Ⅰ by time-resolved fluorescent nanoparticle immunochromatography[J]. Journal of Third Military Medical University, 2017, 39(18): 1803-1807. DOI： 10.16016/j.1000-5404.201702048.

摘要

目的

研制快速定量检测人血清胃蛋白酶原Ⅰ(pepsinogensⅠ，PGⅠ)试纸，并评价试纸性能。

方法

以包裹有时间分辨荧光的纳米微球作为标记示踪物，运用免疫层析试纸技术，研制PGⅠ检测试纸；考察试纸的检测线性、灵敏度、精密度、与对比试剂的符合率。

结果

试纸定量检测的线性良好，方程为Y=0.027 9+0.004 8X，

=0.997 8；灵敏度为0.27 μg/L；批内CV小于10%，批间CV 11.03%；与市售PGⅠ试剂平行检测489份血清样本，有良好的相关性(Y

TRFIA

=0.856 6X

strip

+7.545 6，

=0.989 1

0.01)。

结论

PGⅠ时间分辨荧光纳米微球免疫层析试纸操作简单、快速、经济、可定量，满足临床检测血清PGⅠ的需求。

Abstract

Objective

To develop a fast quantitative detection strip of pepsinogen Ⅰ (PGⅠ).

Methods

With the time-resolved fluorescent nanoparticles as the marker tracer

PGⅠ strip based on double-antibody sandwich immunochromatography was developed. The obtained strip was evaluated for its linearity

sensitivity

accuracy

and consistency with commercially available products (489 serum samples).

Results

The strip showed good linearity

and the linear equation was Y=0.027 9+0.004 8X

=0.997 8. The sensitivity of the PGⅠ strip was 0.27 μg/L. The coefficient of variation (CV) values of intra-batch was less than 10%

and that of inter-batch was 11.03%. Compared with the commercially available product (time-resolved fluorescence immunoassay)

our PGⅠ strip showed the high consistency (Y

TRFIA

=0.856 6X

strip

+7.545 6

=0.989 1

0.01).

Conclusion

Our developed time-resolved fluorescent nanoparticle immunochromatographic strip for PGⅠ detection is rapid

low cost

easy to operate

and quantitative

and can meet the needs of the clinical practice.

关键词

Keywords

references

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