Establishment ofaBALB/c mouse model of melioidosis induced by chronic <italic style="font-style: italic">Burkholderia pseudomallei</italic> infection

Tengfei Ma; Yi Hu; Yao Fang; Pan Zhu; Zhiqiang Hu; Dan Han; Shaoxiong Kang; Qian Li; Xuhu Mao

doi:10.16016/j.1000-5404.201701131

您当前的位置：

首页 >

文章列表页 >

Establishment ofaBALB/c mouse model of melioidosis induced by chronic Burkholderia pseudomallei infection

Basic Medicine | 更新时间：2026-01-09

- Establishment ofaBALB/c mouse model of melioidosis induced by chronic Burkholderia pseudomallei infection
- Journal of Third Military Medical University Vol. 39, Issue 12, Pages: 1239-1244(2017)
- 作者机构：
  
  400038重庆，第三军医大学西南医院医学检验系临床微生物及免疫学教研室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(30973402)
- DOI：10.16016/j.1000-5404.201701131
  CLC： R-332;R378.99;R515.9
- Received：22 January 2017，
  
  Revised：2017-02-28，
  
  Published：30 June 2017
- 稿件说明：
移动端阅览
Tengfei Ma, Yi Hu, Yao Fang, et al. Establishment ofaBALB/c mouse model of melioidosis induced by chronic Burkholderia pseudomallei infection[J]. Journal of Third Military Medical University, 2017, 39(12): 1239-1244.
DOI：

Tengfei Ma, Yi Hu, Yao Fang, et al. Establishment ofaBALB/c mouse model of melioidosis induced by chronic Burkholderia pseudomallei infection[J]. Journal of Third Military Medical University, 2017, 39(12): 1239-1244. DOI： 10.16016/j.1000-5404.201701131.

摘要

目的

建立类鼻疽杆菌(

Burkholderia pseudomallei

)慢性感染BALB/c小鼠模型并评价其稳定性。

方法

采用0.01 mL洗涤后的高、中、低剂量的类鼻疽杆菌液(菌量分别为3.3×10

、3.3×10

CFU)滴鼻感染BALB/c小鼠，密切监测42 d，记录小鼠的生存率，探索合适的攻毒剂量。在低剂量细菌(3.3×10

CFU)感染小鼠后21、28、35、42 d，计数小鼠血液、肝脏、脾脏和肺脏的细菌定植量，ELISA检测血清炎性因子TNF-α、IFN-γ的表达和血清类鼻疽杆菌特异性IgG抗体滴度，观察肝脏、脾脏和肺脏等主要器官的病理学改变，评价动物模型的稳定性。

结果

低剂量类鼻疽杆菌滴鼻感染BALB/c小鼠后，小鼠42 d生存率为60%。期间小鼠体质量呈下降趋势，血液、肝脏、脾脏和肺脏均有细菌定植，并出现大量炎性细胞浸润、多灶性坏死和脓肿等病理改变；类鼻疽杆菌血清中特异性IgG抗体滴度从感染后21 d起持续增高；实验组小鼠血清炎性因子TNF-α和IFN-γ表达明显高于对照组(

0.05)。

结论

成功建立了稳定的类鼻疽杆菌慢性感染BALB/c小鼠模型。

Abstract

Objective

To establishaBALB/c mouse model of melioidosis induced by chronic

Burkholderia pseudomallei

(

pseudomallei

) infection.

Methods

BALB/c mice were challenged with different doses (3.3×10

3.3×10

and 3.3×10

CFU) of

pseudomallei

by intranasal instillation. The mice were monitored daily for 42dto record their survival rate and body weight changes. On days 21

35 and 42 following the infection

the bacterial loads in the blood and organs and the serum levels of tumor necrosis factor-α (TNF-α)

interferon-γ (IFN-γ) and specific anti-

pseudomallei

IgG were detected

and the pathological changes in the liver

panc

reas and lungs of the mice were assessed using HE staining.

Results

Intranasal challenge withalow dose (3.3×10

CFU) of

pseudomallei

resulted inasurvival rate of 60% in the mice on day 42. Chronic

pseudomallei

infection of the mice caused progressive body weight loss and induced obvious pathologies (inflammatory cell infiltration

multiple focal necrosis and abscess) in the liver

pancreas and lungs

where

pseudomallei

colonization was detected. The serum titer of specific

pseudomallei

IgG began to increase continuously from day 21 after the challenge

and the serum levels of TNF-α and IFN-γ were significantly higher in the infected mice than in the control mice.

Conclusion

We have successfully establishedamodel of chronic

pseudomallei

infection in BALB/c mice.

关键词

Keywords

references

毛旭虎.加强类鼻疽的研究[J].第三军医大学学报, 2011, 33(13): 1315-1317.

Mao X H. Progress on melioidosis[J]. J Third Mil Med Univ, 2011, 33(13): 1315-1317.

Soffler C, Bosco-Lauth A M, Aboellail T A, et al . Pathogenesis of percutaneous infection of goats with Burkholderia pseudomallei : clinical, pathologic, and immunological responses in chronic melioidosis[J ] . Int J Exp Pathol, 2014, 95(2): 101-119. DOI:10.1111/iep.12068..

Wiersinga W J, Currie B J, Peacock S J. Melioidosis[J]. N Engl J Med, 2012, 367(11): 1035-1044. DOI:10.1056/NEJMra1204699.

Gauthier Y P, Hagen R M, Brochier G S, et al . Study on the pathophysiology of experimental Burkholderia pseudomallei infection in mice[J ] . FEMS Immunol Med Microbiol, 2001, 30(1): 53-63. DOI:10.1016/s0928-8244(00)00234-0..

Leakey A K, Ulett G C, Hirst R G. BALB/c and C57Bl/6 mice infected with virulent Burkholderia pseudomallei provide contrasting animal models for the acute and chronic forms of human melioidosis[J ] . Microb Pathog, 1998, 24(5): 269-275. DOI:10.1006/mpat.1997.0179.

Fang Y, Huang Y, Li Q, et al . First genome sequence of a Burkholderia pseudomallei Isolate in China, strain BPC006, obtained from a melioidosis patient in Hainan[J ] . J Bacteriol, 2012, 194(23): 6604-6605. DOI:10.1128/JB.01577-12..

Tan G Y, Liu Y, Sivalingam S P, et al . Burkholderia pseudomallei aerosol infection results in differential inflammatory responses in BALB/c and C57Bl/6 mice[J ] . J Med Microbiol, 2008, 57(Pt 4): 508-515. DOI:10.1099/jmm.0.47596-0..

Zhuang Y, Peng L S, Zhao Y L, et al . CD8(+) T cells that produce interleukin-17 regulate myeloid-derived suppressor cells and are associated with survival time of patients with gastric cancer[J ] . Gastroenterology, 2012, 143(4): 951-962. DOI: 10.1053/j.gastro.2012.06.010.

潘金仁, 陈会红, 李寿俊, 等. A群C群脑膜炎球菌多糖结合疫苗6月龄儿童初次免疫效果[J].中国疫苗和免疫, 2015, 21(5): 511-514.

PAN J R, Chen H H, Li S J, et al . Immunogenicity of group A and group C meningococcal conjugate vaccine after primary immunization in children aged 6 months[J ] . Chin J Vaccines Immuniz, 2015, 21(5): 511-514.

Nandi T, Tan P. Less is more: Burkholderia pseudomallei and chronic melioidosis[J ] . MBio, 2013, 4(5): e00709-e00713. DOI:10.1128/mBio.00709-13.

Suttisunhakul V, Wuthiekanun V, Brett P J, et al . Development of rapid enzyme-linked immunosorbent assays for detection of antibodies to Burkholderia pseudomallei [J ] . J Clin Microbiol, 2016, 54(5): 1259-1268. DOI: 10.1128/JCM.02856-15..

Champion O L, Gourlay L J, Scott A E, et al . Immunisat ion with proteins expressed during chronic murine melioidosis provides enhanced protection against disease[J ] . Vaccine, 2016, 34(14): 1665-1671. DOI: 10.1016/j.vaccine.2016.02.038

Pitt T L, Trakulsomboon S, Dance D A B. Recurrent melioidosis: possible role of infection with multiple strains of Burkholderia pseudomallei [J ] . J Clin Microbiol, 2006, 45(2): 680-681. DOI: 10.1128/jcm.01974-06.

Conejero L, Patel N, de Reynal M, et al . Low-dose exposure of C57BL/6 Mice to Burkholderia pseudomallei mimics chronic human melioidosis[J ] . Am J Pathol, 2011, 179(1): 270-280. DOI: 10.1016/j.ajpath.2011.03.031..

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Risk factors for postoperative infection after open wedge high tibial osteotomy: an analysis of 114 cases

Accurately prevent SARS-CoV-2 infections based on the basic principles of infectious disease prevention and control

Clinical value of linked color imaging endoscopy in auxiliary diagnosis of Helicobacter pylori infection

Recombinant expression and immunological characterization of hemolysin coregulated protein 1, Burkholderia pseudomallei type Ⅵ secretion system protein

Hepatitis B virus X promotes tumorigenesis of hepatocellular carcinoma through HIPPO signaling pathway in mice

Related Author

赵加全

简雯雯

丁玉辉

曾维君

李丽娟

张代英

毛青

刘浏

Related Institution

First Clinical College, the First Affiliated Hospital of Chongqing Medical University

Department of Clinical Laboratory, the First Affiliated Hospital of Chongqing Medical University

Department of Clinical Microbiology and Immunology, Faculty of Medical Laboratory Science, Southwest Hospital, Third Military Medical University

⁰

Establishment ofaBALB/c mouse model of melioidosis induced by chronic Burkholderia pseudomallei infection

DOI：10.16016/j.1000-5404.201701131

摘要

Abstract

关键词

Keywords

references