Effects of microglia and macrophages on migration of rat C6 glioma cells

Dongdong Wu; Lunshan Xu; Minhui Xu; Qing Ouyang; Liang Yi

doi:10.16016/j.1000-5404.201701109

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Effects of microglia and macrophages on migration of rat C6 glioma cells

更新时间：2026-01-09

- Effects of microglia and macrophages on migration of rat C6 glioma cells
- Journal of Third Military Medical University Vol. 39, Issue 11, Pages: 1117-1122(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation of China(81172413)
- DOI：10.16016/j.1000-5404.201701109
  CLC：
- Received：19 January 2017，
  
  Published：15 June 2017
- 稿件说明：
移动端阅览
Dongdong Wu, Lunshan Xu, Minhui Xu, et al. Effects of microglia and macrophages on migration of rat C6 glioma cells[J]. Journal of Third Military Medical University, 2017, 39(11): 1117-1122.
DOI：

Dongdong Wu, Lunshan Xu, Minhui Xu, et al. Effects of microglia and macrophages on migration of rat C6 glioma cells[J]. Journal of Third Military Medical University, 2017, 39(11): 1117-1122. DOI： 10.16016/j.1000-5404.201701109.

摘要

Abstract

Objective

To determine the effect of microglia and peripheral blood macrophages on the migration of C6 glioma cells.

Methods

The rat microglial cells and macrophages differentiated from peripheral blood mononuclear cells were co-cultured with C6 glioma cells. Co-cultured with rat fibroblasts served as positive control and singly cultured C6 cells as blank control. Scratch wound healing assay was carried in 0

24 and 48 h after co-culture

and the results were observed in 6 h later. In 24 h and 48 h after co-culture

CD11b

flow cytometry was used to sort and isolate microglia/macrophages and C6 cells. The expression levels of IL-10

IL-18 and MMP-9 in microglia and macrophages and those of TGF-β and FAS ligand in glioma cells were detected by Western blotting when co-cultured for 0

24 and 48 h.

Results

At the initial state

both microglia and macrophages inhibited the migration of glioma

and the inhibitory effect of macrophage was stronger (

0.05). The tumor inhibitory effect of 2 kinds of cells disappeared after co-culture with glioma C6 cells

and all of them showed the effect of promoting tumor migration. The expression levels of IL-10

IL-18 and MMP-9 in the microglia and macrophages were different in the initial stage. But

the levels of these proteins were increased in these cells and consistent after co-culture. The expression levels of TGF-β and FAS ligand were also increased and consistent in glioma cells C6 after co-culture.

Conclusion

Microglia and macrophages are domesticated into the same glioma associated microglia/macrophages after co-culture with C6

and 2 kinds of cells show the same biological effects when co-culture with C6 cells.

关键词

Keywords

references

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