Genotypic characteristics of multidrug-resistant
Mycobacterium tuberculosis in Chongqing- Journal of Third Military Medical University Vol. 39, Issue 12, Pages: 1298-1303(2017)
- 作者机构：
1.400036 重庆，重庆市公共卫生医疗救治中心:检验科
2.400020 重庆，重庆市第十二人民医院检验科
3.400036 重庆，重庆市公共卫生医疗救治中心:耐多药结核病管理办公室
4.400036 重庆，重庆市公共卫生医疗救治中心:结核重点实验室
5.400036 重庆，重庆市公共卫生医疗救治中心:病案室
6.401120 重庆，重庆医科大学附属第三医院骨与创伤中心
- 作者简介：
- 基金信息：Key Project of Chongqing Municipal Health Bureau(2012-1-085)
DOI：10.16016/j.1000-5404.201701077
CLC： R195.4;R372;R378.911Received：12 January 2017，
Revised：2017-02-11，
Published：30 June 2017
- 稿件说明：
移动端阅览
Tongxin Li, Zhenggu Huang, Yiwei Wang, et al. Genotypic characteristics of multidrug-resistant
Mycobacterium tuberculosis in Chongqing[J]. Journal of Third Military Medical University, 2017, 39(12): 1298-1303.Tongxin Li, Zhenggu Huang, Yiwei Wang, et al. Genotypic characteristics of multidrug-resistant
Mycobacterium tuberculosis in Chongqing[J]. Journal of Third Military Medical University, 2017, 39(12): 1298-1303. DOI： 10.16016/j.1000-5404.201701077.
摘要
目的
2
对重庆地区耐多药结核患者结核分枝杆菌进行基因分型，明确该地区基因类型的特征和流行情况。
方法
2
连续收集重庆市公共卫生医疗救治中心和重庆市第十二人民医院结核内科2013年7月至2015年3月753例临床诊断为耐多药结核患者的痰液或其他体液，采用液体培养法分离培养分枝杆菌，采用PCR方法对分枝杆菌分离株进行菌种鉴定，并使用多位点数目可变串联重复序列分析(multiple locus variable number of tandem repeat analysis，MLVA)方法进行基因分型。采用Bionumerics Version 3.0和phyloviz软件进行各基因位点的多态性和成簇分析。
结果
2
共分离培养出538例耐多药分枝杆菌菌株，经PCR鉴定确认结核分枝杆菌有503例(95.8%)，非结核分枝杆菌有35例(4.2%)。北京家族型470例(93.4%，470/503)，非北京家族型33例(6.6%，33/503)。503例分枝杆菌菌株共分为348个基因型，其中267例患者分离株为单一基因型，其余236例菌株可归入81个簇，成簇率为30.8%。北京家族型有76个簇，成簇率为30.8%，成簇比例为35.9%，非北京家族型有5个簇，成簇率为30.3%，成簇比例为33.3%。不同特征的耐多药结核人群对结核分枝杆菌北京基因型菌株易感因素分析：感染北京基因型菌株与性别无显著性关联(χ
2
=2.68
P
>
0.05)；与年龄呈显著性关联(χ
2
=784.00
P
<
0.05)。
结论
2
重庆地区耐多药结核分枝杆菌的基因型存在明显的多态性，北京基因型占绝对优势；不同特征耐多药结核人群感染北京基因型菌株与性别无关
与年龄相关。
Abstract
Objective
2
To investigate the genotypic characteristics and prevalence of multidrug-resistant
Mycobacterium tuberculosis
in Chongqing.
Methods
2
This retrospective study was conducted among 753 patients with a clinical diagnosis of multidrug-resistant tuberculosis
who received treatments in the Public Health Medical Center and the 12th People' s Hospital of Chongqing from July
2013 to March
2015. Mycobacterial strains were isolated from the sputum or other body fluid samples from the patients and cultured using the liquid culture method. The isolates of
Mycobacterium tuberculosis
were tested with PCR and genotyped using multiple-locus variable-number tandem repeat analysis (MLVA). Bionumerics Version 3.0 and Phyloviz software was used to analyze the polymorphisms and clustering of the genetic loci.
Results
2
A total of 538 multidrug-resistant mycobacterial strains were isolated and identified
including 503 (95.8%) strains of
Mycobacterium tuberculosis
and 35 (4.2%) strains of other species. Of the 503 strains of
Mycobacterium tuberculosis
470 (93.4%
470/503) were of the Beijing family genotype and 33 (6.6%
33/503) were of other genotypes. A total of 348 genotypes were identified among the 503 mycobacterial strains; 267 strains had a single genotype while the remaining 236 strains were classified into 81 clusters with a clustering rate of 30.8%. The strains of Beijing family genotype had 76 clusters with a clustering rate of 30.8% and a clustering ratio of 35.9%. Five clusters were identified among the strains with non-Beijing family genotypes with a clustering rate of 30.3% and a clustering ratio of 33.3%. Vulnerability analysis showed that the infection with
Mycobacterium tuberculosis
of Beijing family genotype was significantly correlated with the patients'age (
Chi
s
quare=784.00
P
<
0.05) but not with their gender (
Chi
square=2.68
P
>
0.05).
Conclusion
2
The multidrug-resistant
Mycobacterium tuberculosis
strains isolated from Chongqing have polymorphic genotypes
among which the Beijing family genotype is predominant. The vulnerability to infections by multidrug-resistant
Mycobacterium tuberculosis
of Beijing family genotype is related with age but not with gender.
关键词
Keywords
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