Low molecular heparins exert brain protective effect in MCAO rats by promoting nuclear transfer and SUMO2/3 expression

Xiaofei Gu; Heng Li; Tongju Yang; Kai Wang; Lijun Wang; Zhen Wang; Liyong Chen

doi:10.16016/j.1000-5404.201701065

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Low molecular heparins exert brain protective effect in MCAO rats by promoting nuclear transfer and SUMO2/3 expression

更新时间：2026-01-09

- Low molecular heparins exert brain protective effect in MCAO rats by promoting nuclear transfer and SUMO2/3 expression
- Journal of Third Military Medical University Vol. 39, Issue 11, Pages: 1123-1129(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201701065
  CLC：
- Received：11 January 2017，
  
  Published：15 June 2017
- 稿件说明：
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Xiaofei Gu, Heng Li, Tongju Yang, et al. Low molecular heparins exert brain protective effect in MCAO rats by promoting nuclear transfer and SUMO2/3 expression[J]. Journal of Third Military Medical University, 2017, 39(11): 1123-1129.
DOI：

Xiaofei Gu, Heng Li, Tongju Yang, et al. Low molecular heparins exert brain protective effect in MCAO rats by promoting nuclear transfer and SUMO2/3 expression[J]. Journal of Third Military Medical University, 2017, 39(11): 1123-1129. DOI： 10.16016/j.1000-5404.201701065.

摘要

Abstract

Objective

To determine the effect of low molecular weight heparins (LMWH) on NF-κB activation and expression after cerebral ischemia-reperfusion injury (CIRI) in rats and investigate the possible mechanism.

Methods

Middle cerebral artery occlusion (MCAO) model was established by Longa's method in rats. Then the rats were assigned into 3 groups

that is

sham operation group (Sham

=20)

ischemia reperfusion group (I/R

=20)

LMWH intervention group (LMWH

1.5 mg/kg

intravenous injection

5 min prior to reperfusion

=20). After neurological function was evaluated

the rats were sacrificed in 3 and 24 h after reperfusion

and the brain tissues were harvested for infarct volume. Western blotting

immunohistochemical (IHC) and immunofluorescence (IF) assay were used to detect the expression levels of IL-1β

NF-κB P65 and small ubiquitin-related modifier 2/3 (SUMO2/3).

Results

① Neurological function evaluation showed that MCAO resulted in severe neurological dysfunction in the rats

but LMWH intervention obviously attenuated the injury (

0.01). ② Cerebral I/R injury induced severe cerebral infarction (

0.01)

but

LMWH alleviated the symptoms (

0.01)

and the effects were more significant in the striatum region that was sensitive to infarction (

0.01). ③ Western blot results showed that the protein levels of IL-1β and NF-κB P65 were significantly higher in I/R group than sham operation group in 24 h after reperfusion (

0.01)

while LMWH intervention obviously attenuated the levels. In 3 h after reperfusion

SUMO2/3 protein level was higher in the I/R group than the sham operation group (

0.01). But interestingly

LMWH intervention increased the expression level significantly (

0.01). ④ IHC and IF results showed that after 3 hours' reperfusion

SUMO2/3 protein level was higher in the I/R group than the sham operation group

and nuclear transfer phenomeno

n appeared

mainly occurred in the neurons (

0.01). After LMWH intervention

there were significantly more neurons with SUMO2/3 protein nuclear transfer (

0.01).

Conclusion

LMWH can promote the SUMO2/3 protein level and increase nuclear transfer in rats after CIRI

and then possibly influence the NF-κB activation and reduce the production of inflammatory factors.

关键词

Keywords

references

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