Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children
- Journal of Third Military Medical University Vol. 39, Issue 17, Pages: 1768-1773(2017)
- 作者机构：
1.400014重庆, 重庆医科大学附属儿童医院感染科, 儿童发育疾病研究教育部重点实验室, 儿童发育重大疾病国家国际科技合作基地, 儿科学重庆市重点实验室
2.Department of Epidemiology, School of Public Health, University of Michigan, 1415 Washington Heights, Ann Arbor, MI 48109-2029, USA
- 作者简介：
- 基金信息：
DOI：10.16016/j.1000-5404.201701026
CLC： R738.911;R394.3;R529.9Received：05 January 2017，
Revised：2017-04-18，
Published：15 September 2017
- 稿件说明：
移动端阅览
Tingting MA, Yanfeng HUANG, Zhenhua YANG, et al. Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children[J]. Journal of Third Military Medical University, 2017, 39(17): 1768-1773.
Tingting MA, Yanfeng HUANG, Zhenhua YANG, et al. Gene polymorphisms of esxA, esxH and fbpB in clinical Mycobacterium tuberculosis isolates from tuberculosis children[J]. Journal of Third Military Medical University, 2017, 39(17): 1768-1773. DOI： 10.16016/j.1000-5404.201701026.
摘要
目的
2
对抗原编码基因esxA、fbpB、Rv2660c进行测序
了解esxA、fbpB、Rv2660c的基因多态性。
方法
2
选取135株结核分枝杆菌(
Mycobacterium tuberculosis
MTB)临床分离株
运用PCR方法扩增目的基因esxA、fbpB、Rv2660c并进行测序
与结核标准株H37Rv基因序列以及从免疫表位数据库(immune epitope database
IEDB)中查询到的表位序列进行对比
分析esxA、fbpB、Rv2660c基因序列中的变异特征。
结果
2
在135株菌株中
esxA、Rv2660c序列暂未发现基因变异。fbpB共66株(48.89%)发现了基因变异
5个单核苷酸多态性(single nucleotide polymorphism
SNP)位点共造成8个(14.81%)T细胞抗原表位以及9个(24.32%)B细胞抗原表位序列的多态性。fbpB基因的dN值为0.000 120
dS为0.002 126
其中T细胞表位区与非T细胞表位区的dN值分别为0.000 067和0.000 408
dS分别为0.002 382和0.000 470
B细胞表位区dN和dS值分别为0.000 063和0.002 380
非B细胞表位区dN和dS均为0。
结论
2
esxA、Rv2660c基因序列较保守
fbpB存在基因多态性
预测会对蛋白Ag85B的免疫功能造成影响
从而影响H56的免疫有效性。
Abstract
Objective
2
To sequence the antigen-encoding genes esxA
fbpB and Rv2660c from clinical isolates of
Mycobacterium tuberculosis
(MTB) in tuberculosis children and study the polymorphisms.
Methods
2
A total of 135 isolated strains were amplified for gene sequences of esxA
fbpB and Rv2660c by PCR
and the products were sequenced respectively. The results of sequencing were compared with the corresponding gene sequences of standard strain H37Rv and human T and B cell epitope sequences from immune epitope database (IEDB). The genetic diversities of the gene sequences of esxA
fbpB and Rv2660c were analyzed.
Results
2
Among the 135 strains
no gene polymorphisms were found in esxA and Rv2660c. But fbpB had genetic diversities in 66 clinical isolates (48.89%). The 5 single nucleotide polymorphism (SNP) mutations in fbpB affected 8 (14.81%) T cell epitopes and 9 (24.32%) B cell epitopes. The dN and dS values of whole fbpB gene were 0.000 120 and 0.002 126
respectively. The dN and dS values of T epitopes areas were 0.000 067 and 0.002 382 respectively
those of non-T epitopes area were 0.000 408 and 0.000 470
respectively
those of B epitopes areas were 0.000 063 and 0.00 2380
and those of non-B epitopes areas were 0.
Conclusion
2
The gene sequences of esxA and Rv2660c are conservative
and that of fbpB contains genetic diversities. Its polymorphisms may influence the function of antigen Ag85B
and thus affect the efficacy of vaccine H56.
关键词
Keywords
references
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