Comparative proteomics analysis between mucoid and non-mucoid <italic style="font-style: italic">Pseudomonas aeruginosa</italic> strains

Wenying LUO; Jianjun LIU; Yu DING; Lawei YANG; Xiaohu REN

doi:10.16016/j.1000-5404.201701023

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Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains

Basic Medicine | 更新时间：2026-01-09

- Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains
- Journal of Third Military Medical University Vol. 39, Issue 13, Pages: 1345-1351(2017)
- 作者机构：
  
  1.524001 广东湛江，广东医科大学附属医院检验科
  2.528055 深圳，深圳市疾病预防控制中心毒理实验室
  3.524001 广东湛江，广东医科大学附属医院临床研究中心
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81301478);the Non-funded Scientific Research Project of Zhanjiang City, Guangdong Province(2015B01042);the Foundation for Guangdong Medical Science and Technology Research(B2017055)
- DOI：10.16016/j.1000-5404.201701023
  CLC： R372;R378.991;R394.33
- Received：05 January 2017，
  
  Revised：2017-03-08，
  
  Published：15 July 2017
- 稿件说明：
移动端阅览
Wenying LUO, Jianjun LIU, Yu DING, et al. Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains[J]. Journal of Third Military Medical University, 2017, 39(13): 1345-1351.
DOI：

Wenying LUO, Jianjun LIU, Yu DING, et al. Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains[J]. Journal of Third Military Medical University, 2017, 39(13): 1345-1351. DOI： 10.16016/j.1000-5404.201701023.

摘要

目的

探讨黏液型与非黏液型铜绿假单胞菌(

Pseudomonas aeruginosa

)的差异蛋白谱，以发现黏液型

潜在的生物标志物。

方法

采用来自于临床囊性纤维化患者分离的3株黏液型

菌株，脉冲场凝胶电泳以确定它们的同源性；采用同位素相对和绝对定量标记(isobaric tags for relative and absolute quantitation，iTRAQ)结合二维液相色谱串联质谱(two-dimensional liquid chromatography-tandem mass spectrometry，2D LC-MS/MS)技术分析3株黏液型与对照组1株非黏液型

的差异蛋白谱，并采用RT-qPCR方法对5个共同差异蛋白进行验证。

结果

3株黏液型

菌株不具有同源性，iTRAQ结果没有明显差异。3组共有219个上调和101个下调差异蛋白。pa2815、pa2018、dnak、pa5046和acef基因转录水平与iTRAQ结果一致。

结论

不同黏液表型

的iTRAQ结果没有明显差异，219个上调和101个下调差异蛋白可以作为研究黏液型与非黏液型

致病机制的候选生物标志物。

Abstract

Objective

To investigate the differences in protein profiling between mucoid and non-mucoid

Pseudomonas aeruginosa

(

)

in order to find the potential biological markers of mucoid

Methods

Three strains of mucoid

were isolated from the sputum of cystic fibrosis patients

and then pulsed field gel electrophoresis was used to determine their homology. Isobaric tags for relative and absolute quantitation (iTRAQ) labeling strategy combined with two-dimensional liquid chromatography-tandem mass spectrometry (2D LC-MS/MS) was used to investigate the differential protein abundances between the 3 mucous and 1 non-mucoid

strains. Differential expression of 5 common differential genes was verified by using RT-qPCR.

Results

There was no homology in the 3 mucoid

strains. No significant differences were found in the iTRAQ results of them. There were 219 up-regulated and 101 down-regulated proteins identified among the 3 strains. The transcriptional levels of pa2815

pa2018

dnak

pa5046 and acef genes were consistent with the results of iTRAQ.

Conclusion

There are no significant differences in the iTRAQ results of different mucous

strains. The obtained 219 up-regulated and 101 down-regulated differentially expressed proteins can be used as candidate biomarkers for the study of the pathogenesis of mucoid and n

on-mucoid

关键词

Keywords

references

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Comparative proteomics analysis between mucoid and non-mucoid Pseudomonas aeruginosa strains

DOI：10.16016/j.1000-5404.201701023

摘要

Abstract

关键词

Keywords

references