Phenotype, morphology and function of hepatocellular carcinoma-associated CD33<sup>low</sup>CD11b<sup>low</sup> myeloid cells in hepatocellular carcinoma patients

Wenjie Zhu; Jie Lin; Limin Zheng

doi:10.16016/j.1000-5404.201701018

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Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients

更新时间：2026-01-09

- Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients
- Journal of Third Military Medical University Vol. 39, Issue 11, Pages: 1137-1143(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Major Program of National Natural Science Foundation of China(91442205)
- DOI：10.16016/j.1000-5404.201701018
  CLC：
- Received：04 January 2017，
  
  Published：15 June 2017
- 稿件说明：
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Wenjie Zhu, Jie Lin, Limin Zheng. Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients[J]. Journal of Third Military Medical University, 2017, 39(11): 1137-1143.
DOI：

Wenjie Zhu, Jie Lin, Limin Zheng. Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients[J]. Journal of Third Military Medical University, 2017, 39(11): 1137-1143. DOI： 10.16016/j.1000-5404.201701018.

摘要

Abstract

Objective

To investigate the phenotype

morphology

and function of CD33

low

CD11b

low

myeloid cells in the patients with hepatocellular carcinoma (HCC).

Methods

The phenotype and morphology of CD33

low

CD11b

low

myeloid cells were examined using flow cytometry and Wright-Giemsa staining. The immune functions of CD33

low

CD11b

low

myeloid cells were tested

via

T cell co-culture assay

using freshly isolated CD33

low

CD11b

low

myeloid cells from HCC tissues or tumor supernatant-treated blood CD33

low

CD11b

low

myeloid cells from healthy donors.

Results

Aside from canonical polymorphonuclear neutrophils

there was a subset of CD33

low

CD11b

low

granulocytes in the peripheral blood and tumor tissues in the HCC patients. These cells expressed CD123

CD38 and a low level of HLA-DR

but not CD15

CD16

or CD66b

which resembled a phenotype of basophils. Interestingly

these cells exhibited an immature morphology in HCC patients (

0.001). The frequencies of blood CD33

low

CD11b

low

myeloid cells were significantly higher in the HCC patients than the healthy donors

and these cells were enriched in the tumors. HCC-conditioned CD33

low

CD11b

low

myeloid cells had profound capacity in fostering T cell proliferation.

Conclusion

CD33

low

CD11b

low

myeloid cells

a subset of myeloid cells phenotypically resembled basophils

accumulate in the HCC patients and exhibit an immature morphology

which may contribute to anti-tumor immune response in the tumor microenvironment.

关键词

Keywords

references

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⁰

Phenotype, morphology and function of hepatocellular carcinoma-associated CD33lowCD11blow myeloid cells in hepatocellular carcinoma patients

DOI：10.16016/j.1000-5404.201701018

摘要

Abstract

关键词

Keywords

references

Phenotype, morphology and function of hepatocellular carcinoma-associated CD33^lowCD11b^low myeloid cells in hepatocellular carcinoma patients