Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells

Jiajia TANG; Lin YE

doi:10.16016/j.1000-5404.201612169

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Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells

更新时间：2026-01-09

- Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells
- Journal of Third Military Medical University Vol. 39, Issue 16, Pages: 1643-1647(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201612169
  CLC：
- Received：24 December 2016，
  
  Published：30 August 2017
- 稿件说明：
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Jiajia TANG, Lin YE. Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells[J]. Journal of Third Military Medical University, 2017, 39(16): 1643-1647.
DOI：

Jiajia TANG, Lin YE. Up-regulation of miRNA-671 inhibits proliferation and invasion of gastric cancer cells[J]. Journal of Third Military Medical University, 2017, 39(16): 1643-1647. DOI： 10.16016/j.1000-5404.201612169.

摘要

Abstract

Objective

To investigate the expression of miR-671 in gastric cancer tissues and cell lines

and determine its effects on the proliferation and invasion of gastric cancer cells.

Methods

The expression of miR-671 was detected in gastric cancer tissues and para-carcerous tissues from 8 patients and cell lines SGC7901

MKN28 and MKN45

and human gastric mucosal epithelial cell line GES-1 by quantitative real-time PCR (qRT-PCR). CCK-8 assay was used to measure the cell proliferation after miR-671 up-regulation. The effect of miR-671 on cell migration and invasion was detected by wound healing assay and Transwell chamber assay.

Results

The expression level of miR-671 was significantly decreased in gastric cancer tissues than the adjacent normal tissues (

0.01). The expression levels of miR-671 were significantly decreased in the gastric cancer cell lines compared with GES-1 cells (

0.01). Up-regulation of miR-671 significantly inhibited the proliferation

migration and invasion in gastric cancer cells (

0.01).

Conclusion

miR-671 inhibits proliferation

migration and invasion of gastric cancer cells

and may serve as a tumor suppressor.

关键词

Keywords

references

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