STYK1 down-regulation suppresses colorectal cancer cell growth and invasion <italic style="font-style: italic">in vitro</italic>

Jiajia TANG; Lin YE

doi:10.16016/j.1000-5404.201612168

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STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro

更新时间：2026-01-09

- STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro
- Journal of Third Military Medical University Vol. 39, Issue 17, Pages: 1734-1738(2017)
- 作者机构：
  
  400016 重庆，重庆医科大学附属第一医院重症医学科
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201612168
  CLC： R394.3;R730.23;R735.3
- Received：24 December 2016，
  
  Revised：2017-03-03，
  
  Published：15 September 2017
- 稿件说明：
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Jiajia TANG, Lin YE. STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro[J]. Journal of Third Military Medical University, 2017, 39(17): 1734-1738.
DOI：

Jiajia TANG, Lin YE. STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro[J]. Journal of Third Military Medical University, 2017, 39(17): 1734-1738. DOI： 10.16016/j.1000-5404.201612168.

摘要

目的

探讨STYK1基因下调对结直肠癌细胞HCT-15增殖、凋亡和迁移以及MEK/ERK和PI3K/AKT信号通路的影响。

方法

采用基因干扰技术抑制结直肠癌细胞HCT-15中STYK1的表达，Western blot检测抑制效果，CCK-8检测STYK1表达下调对HC-T15细胞增殖的影响，流式细胞仪检测STYK1表达下调对HC-T15细胞凋亡的影响，Transwell小室实验检测STYK1表达下调对HCT-15细胞侵袭的影响，通过Western blot检测STYK1表达下调对HCT-15细胞MEK/ERK和PI3K/AKT信号通路活性的影响。

结果

与干扰对照组相比，ST

YK1表达下调明显抑制HCT-15细胞增殖活性(

0.01) 和侵袭能力(

0.01)，并诱导细胞凋亡(

0.01)；同时，STYK1表达下调明显抑制HCT-15细胞ERK1/2和AKT蛋白的磷酸水平。

结论

STYK1基因干扰可以抑制结直肠癌细胞的增殖和侵袭，并诱导凋亡，其机制可能与抑制MEK/ERK和PI3K/AKT信号通路活性有关。

Abstract

Objective

To determine the effects of down-regulating serine/threonine/tyrosine kinase 1 (STYK1) on the proliferation

apoptosis and invasion and on MEK/ERK and PI3K/AKT signaling pathways in human colorectal cancer cell line HCT-15.

Methods

The expression of STYK1 protein was down-regulated by RNA interference technique in HCT-15 cells. Then

the STYK1 protein expression was detected by Western blotting. CCK-8 assay was used to measure the effect of STYK1 down-regulation on the proliferation of the cells

flow cytometry was employed to detect the cell apoptosis

and Transwell chamber assay was adopted for cell invasion ability. The expression levels of p-ERK1/2 and p-AKT were detected by Western blotting.

Results

Compared with the siRNA NC group

STYK1 down-regulation significantly inhibited the proliferation (

0.01) and invasion (

0.01)

induced cell apoptosis (

0.01)

and suppressed the expression levels of p-ERK1/2 and p-AKT in the HCT cells transfected with STYK1 siRNA (

0.01).

Conclusion

STYK1 down-regulation inhibits the proliferation and invasion and induces the apoptosis in colorectal cancer cells

which may be related to its inhibition on the MEK/ERK and PI3K/AKT signaling pathways.

关键词

Keywords

references

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STYK1 down-regulation suppresses colorectal cancer cell growth and invasion in vitro

DOI：10.16016/j.1000-5404.201612168

摘要

Abstract

关键词

Keywords

references