Correlation of circular RNA hsa_circ_0029642 with pulmonary hypertension in adult patients with congenital heart disease

Wenlin Peng; Zhao Jian; Chao Liu; Yongsheng Meng; Yun Zhu; Yunhan Jiang; Guiping Luo; Fuqin Tang; Yingbin Xiao

doi:10.16016/j.1000-5404.201612131

您当前的位置：

首页 >

文章列表页 >

Correlation of circular RNA hsa_circ_0029642 with pulmonary hypertension in adult patients with congenital heart disease

Clinical Medicine | 更新时间：2026-02-09

- Correlation of circular RNA hsa_circ_0029642 with pulmonary hypertension in adult patients with congenital heart disease
- Journal of Third Military Medical University Vol. 39, Issue 10, Pages: 1005-1008(2017)
- 作者机构：
  
  400037 重庆，第三军医大学新桥医院全军心血管外科研究所
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201612131
  CLC： R446.11;R541.1;R543.2
- Received：19 December 2016，
  
  Revised：2017-03-08，
  
  Published：30 May 2017
- 稿件说明：
移动端阅览
Wenlin Peng, Zhao Jian, Chao Liu, et al. Correlation of circular RNA hsa_circ_0029642 with pulmonary hypertension in adult patients with congenital heart disease[J]. Journal of Third Military Medical University, 2017, 39(10): 1005-1008.
DOI：

Wenlin Peng, Zhao Jian, Chao Liu, et al. Correlation of circular RNA hsa_circ_0029642 with pulmonary hypertension in adult patients with congenital heart disease[J]. Journal of Third Military Medical University, 2017, 39(10): 1005-1008. DOI： 10.16016/j.1000-5404.201612131.

摘要

目的

检测血清中环状RNA hsa_circ_0029642的含量，明确其与肺动脉高压是否有密切关系，探讨循环环状RNA hsa_circ_0029642作为肺动脉高压血清标志物的可能性。

方法

收集2016年3-7月入院的先天性心脏病伴肺动脉高压患者血清20例(A组)，先天性心脏病不伴肺动脉高压患者血清20例(B组)，风湿性心脏病伴肺动脉高压患者血清20例(C组)，各组从血清中提取总RNA并进行质量检测，进行反转录反应，获得相应的cDNA，以GAPDH作为内参进行标定，进行实时荧光定量PCR反应，根据各样本的定量PCR结果计算RQ值，绘制ROC曲线。

结果

3组样本的总RNA均有较好的质量，A组与C组的RQ值差异无统计学意义(

0.05)，A组与B组的RQ值差异有统计学意义(

0.05)，B组与C组的RQ值差异有统计学意义(

0.01)。即A组和C组的RQ值显著低于B组，而A组与C组的RQ值差异无统计学意义。ROC曲线(曲线下面积AUC=0.752，

0.01，95%

：0.624~0.884)。

结论

先天性心脏病肺动脉高压患者血清中环状RNA hsa_circ_0029642明显下调，循环环状RNA hsa_circ_0029642可作为肺动脉高压新的血清标志物

Abstract

Objective

To investigate the correlation between serum level of circular RNA hsa_circ_0029642 and pulmonary hypertension in adult patients with congenital heart disease and explore the possibility of using hsa_circ_0029642 as a serum marker of pulmonary hypertension.

Methods

Serum samples were collected from 20 patients with congenital heart disease and concurrent pulmonary hypertension (group A)

20 patients with congenital heart disease but without pulmonary hypertension (group B)

and 20 patients with rheumatic heart disease and pulmonary hypertension (group C). The total RNA was extracted from the serum samples for reverse transcription to obtain the corresponding cDNA. Real-time fluorescent quantitative PCR reaction was performed to detect the levels of circular RNA hsa_circ_0029642 with GAPDH as the internal control. The RQ value was calculated and the ROC curve was mapped based on the results of quantitative PCR to assess the value of hsa_circ_0029642 in the diagnosis of pulmonary arterial hypertension.

Results

The total RNA extracted from the serum samples all had good quality in the 3 groups. The RQ value differed significantly between groups A and B (

0.05) and between groups B and group C (

0.01)

but not between groups A and C (

0.05); the RQ value was significantly lower in groups A and C than in group B

and was comparable between groups A and C. Analysis of the ROC curve showed that the serum level of hsa_circ_0029642 had an area under the curve (AUC) of 0.752 (95%

:0.624~0.884

0.01) for the diagnosis of pulmonary arterial hypertension.

Conclusion

The serum level of circular RNA hsa_circ_0029642 is obviously lowered in patients with congenital heart disease and concurrent pulmonary arterial hypertension and can be used as a new serum marker for pulmonary hypertension.

关键词

Keywords

references

Roth T S, Aboulhosn J A. Pulmonary Hypertension and Congenital Heart Disease[J]. Cardiol Clin, 2016, 34(3): 391-400. DOI: 10.1016/j.ccl.2016.04.002.

Montani D, Chaumais M C, Guignabert C, et al. Targeted therapies in pulmonary arterial hypertension[J]. Pharmacol Ther, 2014, 141(2): 172-191. DOI: 10.1016/j. pharmthera.2013.10.002.

D'Alto M, Dimopoulos K, Budts W, et al. Multimodality imaging in congenital heart disease-related pulmonary arterial hypertension[J]. Heart, 2016, 102(12): 910-918. DOI: 10.1136/heartjnl-2015-308903.

Salzman J. Circular RNA Expression: Its Potential Regulation and Function[J]. Trends Genet, 2016, 32(5): 309-316. DOI: 10.1016/j.tig.2016.03.002.

Memczak S, Papavasileiou P, Peters O, et al. Identification and Characterization of Circular RNAs As a New Class of Putative Biomarkers in Human Blood[J]. PLoS ONE, 2015, 10(10): e0141214. DOI: 10.1371/journal.pone.0141214.

D'Alto M, Romeo E, Argiento P, et al. Pulmonary arterial hypertension: the key role of echocardiography[J]. Echocardiography, 2015, 32(Suppl 1): S23-S37. DOI:10.1111/echo.12283.

Qu S, Yang X, Li X, et al. Circular RNA: A new star of noncoding RNAs[J]. Cancer Lett, 2015, 365(2): 141-148. DOI: 10.1016/j.canlet.2015.06.003.

Meng X, Li X, Zhang P, et al. Circular RNA: an emerging key player in RNA world[J]. Briefings in bioinformatics, 2016:bbw045. DOI:10.1093/bib/bbw045.

Hansen T B, Jensen T I, Clausen B H, et al. Natural RNA circles function as efficient microRNA sponges[J]. Nature, 2013, 495(7441): 384-388. DOI: 10.1038/nature11993.

Wang K, Long B, Liu F, et al. A circular RNA protects the heart from pathological hypertrophy and heart failure by targeting miR-223[J]. Eur Heart J, 2016, 37(33): 2602-2611. DOI: 10.1093/eurheartj/ehv713.

Groth A, Vrugt B, Brock M, et al. Inflammatory cytokines in pulmonary hypertension[J]. Respiratory research, 2014, 15(1): 1.

Crosswhite P, Sun Z. Molecular mechanisms of pulmonary arterial remodeling[J]. Mol Med, 2014, 20: 191-201. DOI:10.2119/molmed.2013.00165.

Owens G K, Kumar M S, Wamhoff B R. Molecular regulation of vascular smooth muscle cell differentiation in development and disease[J]. Physiol Rev, 2004, 84(3): 767-801. DOI: 10.1152/physrev.00041.2003.

Fei J, Cui X B, Wang J N, et al. ADAR1-Mediated RNA Editing, A Novel Mechanism Controlling Phenotypic Modulation of Vascular Smooth Muscle Cells[J]. Circ Res, 2016, 119(3): 463-469. DOI: 10.1161/CIRCRESAHA.116.309003.

Rabinovici R, Kabir K, Chen M, et al. ADAR1 is involved in the development of microvascular lung injury[J]. Circ Res, 2001, 88(10): 1066-1071. DOI: 10.1161/hh1001.090877.

Rybak-Wolf A, Stottmeister C, Glažar P, et al. Circular RNAs in the Mammalian Brain Are Highly Abundant, Conserved, and Dynamically Expressed[J]. Mol Cell, 2015, 58(5): 870-885. DOI: 10.1016/j.molcel.2015.03.027.

Xia S J, Tai X T, Li B, et al.Study of circular RNA expression profile of lungs in the mice with hypoxia-induced pulmonary hypertension[J]. Chin J Lung Dis(Electronic Edition), 2015, 8(1): 34-38.

Cheng I K, Ching A K, Chan T C, et al. Reduced CRYL1 expression in hepatocellular carcinoma confers cell growth advantages and correlates with adverse patient prognosis[J]. J Pathol, 2010, 220(3): 348-360. DOI: 10.1002/path.2644.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Role of survivin in pulmonary hypertension of congenital heart disease

Surgical treatment for congenital heart disease complicated with infective endocarditis

Risk prediction for severe pneumonia following congenital heart disease surgery in children

Role of LIGHT in development of hypoxic pulmonary hypertension in mice

Clinically relevant risk factors for acute kidney injury after pediatric congenital heart surgery

Related Author

郝嘉

周建国

肖颖彬

唐旭东

Wencheng PAN

Yong WANG

Yingbin XIAO

Wei WANG

Related Institution

PLA Institute of Cardiovascular Surgery, Second Affiliated Hospital, Army Medical University (Thrid Military Medical University)

Department of Cardiothoracic Surgery, Key Laboratory of Child Development and Disorders of Ministry of Education, Children's Hospital of Chongqing Medical University

Department of High Altitude Physiology and Pathology

Department of High Altitude Special Drug and Equipment Research, Key Laboratory of High Altitude Medicine of PLA, College of High Altitude Military Medicine

Department of Immunology, College of Basic Medical Sciences, Army Medical University (Third Military Medical University)

⁰