Effect of down-regulation of UHRF1 on proliferation and invasive capacity in human laryngeal carcinoma Hep-2 cells

Taoyu WEN; Lin ZHU; Yanan LIU; Jie WAN; Yue LEI; Hongyan CHEN

doi:10.16016/j.1000-5404.201612122

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Effect of down-regulation of UHRF1 on proliferation and invasive capacity in human laryngeal carcinoma Hep-2 cells

Basic Medicine | 更新时间：2026-01-09

- Effect of down-regulation of UHRF1 on proliferation and invasive capacity in human laryngeal carcinoma Hep-2 cells
- Journal of Third Military Medical University Vol. 39, Issue 13, Pages: 1339-1344(2017)
- 作者机构：
  
  1.400016 重庆，重庆医科大学附属第一医院耳鼻喉科
  2.646000 四川泸州，西南医科大学附属医院耳鼻咽喉头颈外科
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81272980);the National Clinical Key-discipline Construction Program of China(〔2012〕649)
- DOI：10.16016/j.1000-5404.201612122
  CLC： R73-37;R730.23;R739.65
- Received：18 December 2016，
  
  Revised：2017-02-12，
  
  Published：15 July 2017
- 稿件说明：
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Taoyu WEN, Lin ZHU, Yanan LIU, et al. Effect of down-regulation of UHRF1 on proliferation and invasive capacity in human laryngeal carcinoma Hep-2 cells[J]. Journal of Third Military Medical University, 2017, 39(13): 1339-1344.
DOI：

Taoyu WEN, Lin ZHU, Yanan LIU, et al. Effect of down-regulation of UHRF1 on proliferation and invasive capacity in human laryngeal carcinoma Hep-2 cells[J]. Journal of Third Military Medical University, 2017, 39(13): 1339-1344. DOI： 10.16016/j.1000-5404.201612122.

摘要

目的

探讨下调泛素样含PHD和环指域1(ubiquitin-like protein containing PHD and RING finger domains 1，UHRF1) 基因在喉癌Hep-2细胞中的表达后对其增殖，凋亡，侵袭迁移能力的影响。

方法

实验分为空白组，阴性对照组和干扰组。利用UHRF1-siRNA干扰UHRF1在喉癌Hep-2细胞中的表达，采用qRT-PCR、Western blot检测UHRF1的表达变化。采用MTT法、流式细胞术、Transwell实验检测下调UHRF1表达后Hep-2细胞的增殖、凋亡、侵袭、迁移能力的变化。Western blot检测下调UHRF1表达后Hep-2细胞内Bax、Bcl-2、MMP-2、MMP-9蛋白表达的变化情况。

结果

与空白组和阴性对照组相比干扰组下调UHRF1的表达后可明显抑制喉癌Hep-2细胞的增殖能力(

＜0.001)。空白组，阴性对照组，干扰组凋亡率分别为(5.47±2.77)%，(3.95±0.66)%，(18.95±1.10)%，干扰组凋亡率明显提高(

＜0.001)。空白组，阴性对照，干扰组每视野侵袭细胞数分别为(213.7±13.1)，(221.3±10.3)，(97.7±7.5)，干扰组侵袭细胞数明显降低(

＜0.001)。空白组，阴性对照，干扰组每视野迁移细胞数分别为(230.7±5.5)，(222.7±11.2)，(111.7±7.6)，干扰组迁移细胞数明显降低(

＜0.001)。干扰组细胞Bax蛋白表达上调(

＜0.001)，Bcl-2蛋白表达下调(

＜0.001)，MMP-2蛋白、MMP-9蛋白表达下调(

＜0.001)。

结论

在喉癌Hep-2细胞中下调UHRF1的表达后可明显抑制细胞的增殖，促进细胞凋亡，抑制细胞的侵袭迁移能力。

Abstract

Objective

To determine the effect of down-regulation of ubiquitin-like protein containing PHD and ring finger domains 1 (UHRF1) on the proliferation

apoptosis

invasion and migration in human laryngeal carcinoma Hep-2 cell line.

Methods

The Hep-2 cells were randomly divided into 3 groups: blank control group

negative control group and tranfected group. UHRF1-siRNA was used to down-regulate the expression of UHRF1 in the Hep-2 cells

and quantitative real-time PCR (qRT-PCR) and Western blotting were used to detect the mRNA and protein expression of UHRF1 after down-regulation. The proliferative

invasive and migratory capabilities of Hep-2 cells transfected with UHRF1-siRNA were detected by MTT assay

flow cytometry and Transwell chamber assay. Western blotting was used to measure the protein levels of Bcl-2

Bax

MMP-2 and MMP-9.

Results

Compared with the blank control group and negative control group

down-regulation of UHRF1 significantly inhibited the proliferative capabilities of Hep-2 cells (

0.001). The apoptotic rate was (5.47±2.77)%

(3.95±0.66)% and (18.95±1.10)%

respectively in the blank control group

negative control group and transfected group

and the rate w

as the highest in the transfected group. The number of invasive cells was (213.7±13.1)

(221.3±10.3) and (97.7±7.5) respectively

and that of migratory cells was (230.7±5.5)

(222.7±11.2) and (111.7±7.6) respectively in the above 3 groups. The numbers of invasive and migratory cells were both obviously lower in the transfected group (

0.001). Western blotting results showed that Bax was up-regulated (

0.001)

and Bcl-2

MMP-2 and MMP-9 were down-regulated (

0.001).

Conclusion

Down-regulation of UHRF1 inhibits the proliferation

induces the apoptosis

and reduces the invasive and migratory capability in Hep-2 cells.

关键词

Keywords

references

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