Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism

Xinhua GONG; Qicheng ZHOU; Yeyun GU; Jundong ZHU; Mantian MI

doi:10.16016/j.1000-5404.201612114

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Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism

更新时间：2026-01-09

- Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism
- Journal of Third Military Medical University Vol. 39, Issue 16, Pages: 1612-1617(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81372975)
- DOI：10.16016/j.1000-5404.201612114
  CLC：
- Received：15 December 2016，
  
  Published：30 August 2017
- 稿件说明：
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Xinhua GONG, Qicheng ZHOU, Yeyun GU, et al. Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(16): 1612-1617.
DOI：

Xinhua GONG, Qicheng ZHOU, Yeyun GU, et al. Dihydromyricetin improves cardiac function in ob/ob mice and its underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(16): 1612-1617. DOI： 10.16016/j.1000-5404.201612114.

摘要

Abstract

Objective

To determine the protective effect of dihydromyricetin (DHM) on cardiac function in ob/ob mice (with leptin mutation) and investigate the underlying mechanism.

Methods

Thirty-six male ob/ob mice were randomly divided into 3 groups: ob/ob group

ob/ob+DHM 50 mg/kg group

ob/ob+DHM 100 mg/kg group (

=12). Ten C57BL/6J wild type mice are used as normal control (WT). Glucose tolerance and insulin tolerance were measured after 16 weeks' treatment. Cardiac function was detected by echocardiography. Myocardial histopathology and collagen deposition were detected by HE and Masson staining. The protein expression levels o

f peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PGC-1α) and fibronectin typeⅢ domain-containing protein 5 (FNDC5) in the myocardium were detected by Western blotting. The expression of FNDC5 in the myocardium was observed by immunohistochemical assay.

Results

Glucose tolerance and insulin tolerance were obviously injured in the ob/ob mice than the WT group (

0.05). In the ob/ob mice

no changes were found in diastolic left ventricular diameter (LVIDd)

systolic left ventricular diameter (LVIDs)

or ratio of velocity of early filling wave (E) to velocity of late filling wave due to atrial contraction (A) (

0.05)

or myocardial hypertrophy or collagen deposition

but notable decreases were found in short axis fractional shortening (FS) and ejection fraction (EF) (

0.05)

as well as the protein expression levels of PGC-1α and FNDC5 (

0.05). DHM treatment for 16 weeks could significantly reverse the above changes in the ob/ob mice (

0.05).

Conclusion

DHM ameliorates cardiac function in the ob/ob mice

which might be related to PGC-1α/FNDC5 signalling pathway.

关键词

Keywords

references

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