Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway

Yeyun GU; Qicheng ZHOU; Xinhua GONG; Jundong ZHU; Mantian MI

doi:10.16016/j.1000-5404.201612097

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Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway

更新时间：2026-01-09

- Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway
- Journal of Third Military Medical University Vol. 39, Issue 16, Pages: 1606-1611(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Supported by the General Program of National Natural Science Foundation of China(81372975)
- DOI：10.16016/j.1000-5404.201612097
  CLC：
- Received：14 December 2016，
  
  Published：30 August 2017
- 稿件说明：
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Yeyun GU, Qicheng ZHOU, Xinhua GONG, et al. Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway[J]. Journal of Third Military Medical University, 2017, 39(16): 1606-1611.
DOI：

Yeyun GU, Qicheng ZHOU, Xinhua GONG, et al. Dihydrokaempferol derivatives inhibit palmitic acid-induced lipid deposition in C2C12 myotubes through AMPK/PGC-1α pathway[J]. Journal of Third Military Medical University, 2017, 39(16): 1606-1611. DOI： 10.16016/j.1000-5404.201612097.

摘要

Abstract

Objective

To determine the effects of 4 derivatives from dihydrokaempferol on lipid deposition in C2C12 myotubes (C2C12-MD cells) and investigate the underlying mechanisms.

Methods

Mouse C2C12 myoblasts were differentiated into myotubes and then treated by palmitic acid (PA) alone or in combination with 4 derivatives of dihydrokaempferol (kaempferol

dihydromyricetin

myricetin and quercetin) respectively. Lipid deposition was evaluated by oil red O staining and triglyceride (TG) content measurement. Glucose uptake was measured by using 2-NBDG fluorescent probe. The protein levels of AMP-activated protein kinase (AMPK)

phosphorylated AMPK (p-AMPK) and peroxisome proliferator activator receptor gamma coactivator-1alpha (PGC-1α) were detected by Western blotting.

Results

PA induced more lipid deposition

significantly increased TG content

and significantly decreased glucose uptake under insulin stimul

ation as well as the protein levels of p-AMPK and PGC-1α in C2C12 myotubes (

0.05). Kaempferol treatment had no significant effects on these changes induced by PA. However

the other 3 derivatives markedly inhibited these changes induced by PA

and dihydromyricetin showed a stronger inhibitory effect (

0.05). Moreover

AMPK inhibitor compound C could significantly reverse the inhibitory effect of dihydromyricetin (

0.05).

Conclusion

Three derivatives of dihydrokaempferol (dihydromyricetin

myricetin and quercetin) inhibit PA-induced lipid deposition in C2C12-MD cells through AMPK/PGC-1α signaling pathway

and thereby improve insulin resistance.

关键词

Keywords

references

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