Anti-cancer effect of active gingerol ingredients from ginger <italic style="font-style: italic">in vitro</italic> and underlying mechanism

Xin Liu; Hongwei Zhang; Ruoqiu Fu; Ning Gao

doi:10.16016/j.1000-5404.201612059

您当前的位置：

首页 >

文章列表页 >

Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism

更新时间：2026-01-09

- Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 884-890(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81202869)
- DOI：10.16016/j.1000-5404.201612059
  CLC：
- Received：09 December 2016，
  
  Published：15 May 2017
- 稿件说明：
移动端阅览
Xin Liu, Hongwei Zhang, Ruoqiu Fu, et al. Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(9): 884-890.
DOI：

Xin Liu, Hongwei Zhang, Ruoqiu Fu, et al. Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism[J]. Journal of Third Military Medical University, 2017, 39(9): 884-890. DOI： 10.16016/j.1000-5404.201612059.

摘要

Abstract

Objective

To extract

isolate and identify gingerols from ginger

determine their anti-cancer effect and investigate the underlying mechanism.

Methods

Supercritical CO

fluid extraction was used to extract ginger. The products were then isolated and purified by silica gel

RP-C

Sephadex LH-20 and high performance liquid chromatography (HPLC)

and identified by mass spectrometry (MS) and nuclear magnetic resonance (NMR) spectroscopy. The inhibitory effects of the obtained gingerols on cell proliferation in several cancer cell lines were evaluated by MTT assay. Flow cytometry and Western blotting were employed to determine the cell cycle and the expression of relevant proteins.

Results

There were 5 gingerols isolated from ginger

that is

4-gingerol

6-gingerol

8-gingerol

10-gingerol and 5′-hydroxy-6-gingerol. Among them

8-gingerol

and especially

10-gingerol exhibited potential inhibitory effects on the cell proliferation in 5 different kinds of cancer cells. The inhibitory concentrations of IC

were 25.80±1.39 and 35.29±2.70 μmol/L

respectively in the breast cancer MDA-MB-231 and MCF-7 cells after 72 hours' treatment with 10-gingerol. Flow cytometry found that 8-gingerol and 10-gingerol resulted in cell cycle arrest at G

phase

and the propor

tion of G

phase cells was (66.73±2.93)% and (66.59±2.49)%

respectively in the 2 breast cell lines after 10-gingerol treatment for 24 h

significantly higher than in the cells without treatment [(47.39±1.97)%

(49.17±3.52)%

0.01

]

. Western blotting indicated that 8-gingerol and 10-gingerol down-regulated the expression levels of G

phase related proteins

Cyclin D and CDK4 and decreased p-ERK and enhanced p-P38 in MAPK signaling pathway in MDA-MB-231 and MCF-7 cells.

Conclusion

8-gingerol and 10-gingerol derived from ginger exhibit obviously inhibitory effects on cancer cell proliferation

which are associated with its interruption of ERK and p38 MAPK signaling pathway and then leading to G

phase arrest.

关键词

Keywords

references

张贵君中药鉴定学北京科学出版社 2002 张贵君.中药鉴定学[M].北京:科学出版社, 2002.

Zhang G J Authentication of Chinese Medicine Beijing Science Press 2002 Zhang G J. Authentication of Chinese Medicine[M].Beijing: Science Press, 2002.

李时珍本草纲目 2版北京人民卫生出版社 2004 李时珍.本草纲目[M].2版.北京:人民卫生出版社, 2004.

Li S Z Compendium of Materia Medica 2nd ed Beijing People's Medical Publishing House 2004 Li S Z.Compendium of Materia Medica[M]. 2nd ed. Beijing: People's Medical Publishing House, 2004.

姜程曦林良义宋娇姜中姜酚和姜醇的研究进展中草药 2015 46 16 2499 2504 10.7501/j.issn.0253-2670.2015.16.027 姜程曦, 林良义, 宋娇, 等.姜中姜酚和姜醇的研究进展[J].中草药, 2015, 46(16):2499-2504. DOI:10.7501/j.issn.0253-2670.2015.16.027.

Jiang C X Lin L Y Song J Research progress on gingerol and zingiberol from ginger Chinese Traditional and Herbal Drugs 2015 46 16 2499 2504 10.7501/j.issn.0253-2670.2015.16.027 Jiang C X, Lin L Y, Song J, et al.Research progress on gingerol and zingiberol from ginger[J].Chinese Traditional and Herbal Drugs, 2015, 46(16):2499-2504. DOI:10.7501/j.issn.0253-2670.2015.16.027.

Baliga M S Haniadka R Pereira M M Update on the chemopreventive effects of ginger and its phytochemicals Crit Rev Food Sci Nutr 2011 8 4 265 273 10.3109/1547691X.2011.590828 Baliga, M S, Haniadka R, Pereira M M, et al. Update on the chemopreventive effects of ginger and its phytochemicals[J]. Crit Rev Food Sci Nutr, 2011, 8(4):265-273.DOI:10.3109/1547691X.2011.590828.

Dugasani S Pichika M R Nadarajah V D Comparative antioxidant and anti-inflammatory effects of Ethnopharmacol 2010 127 2 515 520 10.1016/j.jep.2009.10.004 Dugasani S, Pichika M R, Nadarajah V D, et al. Comparative antioxidant and anti-inflammatory effects of[6]-gingerol, [8]-gingerol, [10]-gingerol and[6]-shogaol[J]. Ethnopharmacol, 2010, 127(2):515-520.DOI:10.1016/ j.jep.2009.10.004.

Bernard M Furlong S J Power Coombs M P R Differential inhibition of T lymphocyte proliferation and cytokinesynthesis by Phytother Res 2015 29 11 1707 1713 10.1002/ptr.5414 Bernard M, Furlong S J, Power Coombs M P R, et al. Differential inhibition of T lymphocyte proliferation and cytokinesynthesis by[6]-gingerol, [8]-gingerol, and[10]-gingerol[J]. Phytother Res, 2015, 29(11): 1707-1713.DOI:10.1002/ptr.5414.

Swapna S R Kadimi U S Characterization of gingerol analogues in supercritical carbon dioxide (SC CO 2 ) extract of ginger ( Zingiber officinale, R ., ) J Food Sci Technol 2014 51 11 3383 3389 10.1007/s13197-012-0851-4 Swapna S R, Kadimi U S. Characterization of gingerol analogues in supercritical carbon dioxide (SC CO 2 ) extract of ginger ( Zingiber officinale, R ., )[J ] . J Food Sci Technol, 2014, 51(11):3383-3389.DOI: 10.1007/s13197-012-0851-4.

曾凡逵黄雪松李爱军超临界二氧化碳萃取姜油树脂与溶剂浸提的比较食品科学 2006 27 6 155 157 10.3321/j.issn:1002-6630.2006.06.032 曾凡逵, 黄雪松, 李爱军.超临界二氧化碳萃取姜油树脂与溶剂浸提的比较[J].食品科学, 2006, 27(6):155-157.DOI: 10.3321/j.issn:1002-6630.2006.06.032.

Zeng F K Huang X S Li A J Ginger Oleoresins Extraction with SFE-CO2 in Comparison with Ethanol and Acetone Food Science 2006 27 6 155 157 10.3321/j.issn:1002-6630.2006.06.032 Zeng F K, Huang X S, Li A J. Ginger Oleoresins Extraction with SFE-CO2 in Comparison with Ethanol and Acetone[J].Food Science, 2006, 27(6):155-157.DOI: 10.3321/j.issn:1002-6630.2006.06.032.

Gan Z Liang Z Chen X Separation and preparation of 6-gingerol from molecular distillation residue of Yunnan ginger rhizomes by high-speed counter-current chromatography and the antioxidant activity of ginger oils in vitro J Chromatogr B Analyt Technol Biomed Life Sci 2016 1011 99 107 10.1016/j.jchromb.2015.12.051 Gan Z, Liang Z, Chen X, et al. Separation and preparation of 6-gingerol from molecular distillation residue of Yunnan ginger rhizomes by high-speed counter-current chromatography and the antioxidant activ ity of ginger oils in vitro [J ] . J Chromatogr B Analyt Technol Biomed Life Sci, 2016, 1011:99-107.DOI: 10.1016/j.jchromb.2015.12.051.

Schoenknecht C Andersen G Schmidts I Quantitation of Gingerols in Human Plasma by Newly Developed Stable Isotope Dilution Assays and Assessment of Their Immunomodulatory Potential J Agric Food Chem 2016 64 11 2269 2279 10.1021/acs.jafc.6b00030 Schoenknecht C, Andersen G, Schmidts I, et al. Quantitation of Gingerols in Human Plasma by Newly Developed Stable Isotope Dilution Assays and Assessment of Their Immunomodulatory Potential[J]. J Agric Food Chem, 2016, 64(11):2269-2279.DOI: 10.1021/acs.jafc.6b00030.

Lee S W Lim J H Kim M S Phenolic compounds isolated from Zingiberofficinale roots inhibit cell adhesion Food Chemistry 2011 128 3 778 782 10.1016/j.foodchem.2011.03.095 Lee S W, Lim J H, Kim M S, et al. Phenolic compounds isolated from Zingiberofficinale roots inhibit cell adhesion[J]. Food Chemistry, 2011, 128(3):778-782. DOI:10.1016/j. foodchem.2011.03.095.

Kim J S Lee S I Park H W Cytotoxic components from the dried rhizomes of Zingiberofficinale Roscoe Arch Pharm Res 2008 31 4 415 418 10.1007/s12272-001-1172-y Kim J S, Lee S I, Park H W, et al. Cytotoxic components from the dried rhizomes of Zingiberofficinale Roscoe[J]. Arch Pharm Res, 2008, 31(4):415-418.DOI: 10.1007/s12272-001-1172-y.

Chen C Y Yeh Y T Two new phenylalkanoids from the rhizomes of Zingiber officinale Nat Prod Res 2011 25 1 62 67 10.1080/14786419.2010.490917 Chen C Y, Yeh Y T. Two new phenylalkanoids from the rhizomes of Zingiber officinale[J]. Nat Prod Res, 2011, 25(1):62-67. DOI: 10.1080/14786419.2010.490917.

陈燕闫红蔡同一生姜精深加工的研究农业工程学报 2001 17 3 107 110 http://www.cnki.com.cn/Article/CJFDTOTAL-NYGU200103024.htm 陈燕, 闫红, 蔡同一, 等.生姜精深加工的研究[J].农业工程学报, 2001, 17(3): 107-110.

Chen Y Yan H Cai T Y Study on deep processing and utilization of ginger Transactions of the CSAE 2001 17 3 107 110 http://www.cnki.com.cn/Article/CJFDTOTAL-NYGU200103024.htm Chen Y, Yan H, Cai T Y, et al. Study on deep processing and utilization of ginger[J]. Transactions of the CSAE, 2001, 17(3): 107-110.

Oyagbemi A A Saba A B Azeez O I Molecular targets of Biofactors 2010 36 3 169 178 10.1002/biof.78 Oyagbemi A A, Saba A B, Azeez O I. Molecular targets of[6]-gingerol: Its potential roles in cancer chemoprevention[J].Biofactors, 2010, 36(3):169-178. DOI: 10.1002/biof.78.

Shukla Y Singh M Cancer preventive properties of ginger: A brief review Food Chem Toxicol 2007 45 5 683 690 10.1016/j.fct.2006.11.002 Shukla Y, Singh M. Cancer preventive properties of ginger: A brief review[J]. Food Chem Toxicol, 2007, 45(5):683-690.DOI: 10.1016/j.fct.2006.11.002.

Lee H S Seo E Y Kang N E [6]-Gingerol inhibits metastasis of MDA-MB-231 human breast cancer cells J Nutr Biochem 2008 19 5 313 319 10.1016/j.jnutbio.2007.05.008 Lee H S, Seo E Y, Kang N E, et al.[6]-Gingerol inhibits metastasis of MDA-MB-231 human breast cancer cells[J].J Nutr Biochem, 2008, 19(5):313-319.DOI: 10.1016/j.jnutbio.2007.05.008.

Kim M O Lee M H Oi N [6]-shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2 Carcinogenesis 2014 35 3 683 691 10.1093/carcin/bgt365 Kim M O, Lee M H, Oi N, et al.[6]-shogaol inhibits growth and induces apoptosis of non-small cell lung cancer cells by directly regulating Akt1/2[J]. Carcinogenesis, 2014, 35(3):683-691. DOI: 10.1093/carcin/bgt365.

Fan J Yang X Bi Z 6-Gingerol inhibits osteosarcoma cell proliferation through apoptosis and AMPK activation Tumour Biol 2015 36 2 1135 1141 10.1007/s13277-014-2723-1 Fan J, Yang X, Bi Z.6-Gingerol inhibits osteosarcoma cell proliferation through apoptosis and AMPK activation[J].Tumour Biol, 2015, 36(2):1135-1141.DOI: 10.1007/s13277-014-2723-1.

Sang S Hong J Wu H Increased growth inhibitory effects on human cancer cells and anti-inflammatory potency of shogaols from Zingiberofficinale relative to gingerols J Agric Food Chem 2009 57 22 10645 10650 10.1021/jf9027443 Sang S, Hong J, Wu H, et al. Increased growth inhibitory effects on human cancer cells and anti-inflammatory potency of shogaols from Zingiberofficinale relative to gingerols[J]. J Agric Food Chem, 2009, 57(22):10645-10650.DOI: 10.1021/jf9027443.

Kim S M Kim C Bae H 6-Shogaol exerts anti-proliferative and pro-apoptotic effects through the modulation of STAT3 and MAPKs signaling pathways Mol Carcinog 2015 54 10 1132 1146 10.1002/mc.22184 Kim S M, Kim C, Bae H, et al. 6-Shogaol exerts anti-proliferative and pro-apoptotic effects through the modulation of STAT3 and MAPKs signaling pathways[J]. Mol Carcinog, 2015, 54(10):1132-1146. DOI: 10.1002/mc.22184.

Views

下载量

CSCD

Alert me when the article has been cited

提交

Tools

Publicity Resources

Abnormal proliferation of Leydig cells in Sox30 knockout mice

Microarray analysis of the mechanism of liver injury induced by acute cadmium exposure in rats

MiR-143 regulates proliferation and apoptosis of SKM-1 cells in vitro by inhibiting AF9

Effect of LncRNA LINC01140 on proliferation and cell cycle in acute myeloid leukemia U937 cells

Related Author

Xiuhua CAI

Anying DONG

Ronghua ZHANG

Ganping BAI

Ping WAN

Yi JIANG

Qi ZHU

Ying TANG

Related Institution

Department of Traditional Chinese Medicine and Rheumatology, First Affiliated Hospital, Army Medical University (Third Military Medical University)

Department of Heart, Kidney and Respiratory Diseases, No.951 Hospital of PLA, Korla, Xinjiang Uygur Autonomous Region

Department of Occupational Health and Environmental Hygiene, School of Public Health and Management, Ningxia Medical University, Yinchuan, Ningxia Hui Autonomous Region

Institute of Toxicology, Faculty of Military Preventive Medicine, Army Medical University (Third Military Medical University)

College of Pharmaceutical Sciences and Chinese Medicine, Southwest University

⁰

Anti-cancer effect of active gingerol ingredients from ginger in vitro and underlying mechanism

DOI：10.16016/j.1000-5404.201612059

摘要

Abstract

关键词

Keywords

references