Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus

Li He; Yangang Zhao; Linli Qiu; Yuanyuan Zhang; Jikai Zhao; Fangzhou Xing; Jiqiang Zhang; Wei Li

doi:10.16016/j.1000-5404.201612048

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Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus

更新时间：2026-01-09

- Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 852-858(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81571059)
- DOI：10.16016/j.1000-5404.201612048
  CLC：
- Received：07 December 2016，
  
  Published：15 May 2017
- 稿件说明：
移动端阅览
Li He, Yangang Zhao, Linli Qiu, et al. Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus[J]. Journal of Third Military Medical University, 2017, 39(9): 852-858.
DOI：

Li He, Yangang Zhao, Linli Qiu, et al. Estrogen receptor beta regulates actin polymerization related proteins in mouse hippocampus[J]. Journal of Third Military Medical University, 2017, 39(9): 852-858. DOI： 10.16016/j.1000-5404.201612048.

摘要

Abstract

Objective

To detect the expression profile of estrogen receptor β (ERβ) in the postnatal hippocampus of male and female mice during development and investigate the underlying mechanism of ERβ activity on the regulation of actin polymerization related proteins.

Methods

C57BL/6 mice were divided into 10 groups by their sex and postnatal days (P0

P28 and P56). The expression of ERβ in the hippocampus was determined with immunohistochemical assay and Western blotting. Then adult female diestrus C57BL/6 mice were randomly divided into 5 groups

that is

control and PHTPP (ERβ inhibitor) treatment groups (1

5 and 7 d). DMSO and PHTPP (100 μg/kg) were intra-peritoneally injected. Then another 5 groups were treated respectively with DMSO

ovariectomy (OVX)

and DPN (ERβ activator

1.25

2.5 or 5.0 mg/kg) subcutaneously in 1 week after OVX. Immunohistochemical assay was used to examine the expression of steroid receptor coactivator-1 (SRC-1)

and Western blotting was employed to detect the expression of hippocampal Rictor

phospho-protein kinase B (p-Akt)

Profilin-1

phospho-cofilin ser3 (p-cofilin) and SRC-1.

Results

In both male and female hippocampus

high ERβ level was found at P0

then the level was decreased to the lower level at P7 and P14 (

0.05 compared with P0) and increased to the higher level at P28 and P56 (

0.01 compared with P7 and P14). In the adult mice after 7 days' PHTPP treatment

the expression levels of Rictor

p-Akt

Profilin-1

p-cofilin and SRC-1 were significantly decreased (

0.05). On the contrary

these levels was decreased dramatically after OVX but were significantly rescued by the 7 days' DPN treatment (

0.05).

Conclusion

Postnatal ERβ profile is in a U-shape trend in the hippocampus of mice. Manipulation of ERβ activity

Results

in the alternations of SRC-1 and actin cytoskeleton polymerization. This indicates ERβ may mediate estrogens regulation on learning and memory through SRC-1/Rictor/p-Akt pathway.

关键词

Keywords

references

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