Chronic hypoxia induces myocardial autophagy in mice

Chao Liu; Zhao Jian; Yongsheng Meng; Wenlin Peng; Yun Zhu; Yunhan Jiang; Chao Luo; Fuqin Tang; Yingbin Xiao

doi:10.16016/j.1000-5404.201611246

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Chronic hypoxia induces myocardial autophagy in mice

更新时间：2026-01-09

- Chronic hypoxia induces myocardial autophagy in mice
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 859-864(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81270228)
- DOI：10.16016/j.1000-5404.201611246
  CLC：
- Received：15 November 2016，
  
  Published：15 May 2017
- 稿件说明：
移动端阅览
Chao Liu, Zhao Jian, Yongsheng Meng, et al. Chronic hypoxia induces myocardial autophagy in mice[J]. Journal of Third Military Medical University, 2017, 39(9): 859-864.
DOI：

Chao Liu, Zhao Jian, Yongsheng Meng, et al. Chronic hypoxia induces myocardial autophagy in mice[J]. Journal of Third Military Medical University, 2017, 39(9): 859-864. DOI： 10.16016/j.1000-5404.201611246.

摘要

Abstract

Objective

To determine the effect of chronic hypoxia on myocardial autophagy in mice and investigate the role of AMPKα2 knockout during the process.

Methods

Wild-type (WT) and AMPKα2 knockout (KO) male C57 mice were randomly divided into WT normoxic group

WT hypoxic group

KO normoxic group and KO hypoxic group

with 10 mice in each group. The normoxic groups were kept in normoxic condition

while the hypoxic groups were kept in a hypoxic chamber with 10% O

for 4 weeks. Then the blood samples of WT mice were collected for detecting the levels of red blood cells and hemoglobins. Heart samples of WT mice were harvested to detect the expression of AMPKα2 and p62 and ratio of autophagy-related protein LC3-Ⅱ/LC3-Ⅰ by Western blotting. The change of LC3 level was also detected by immunofluorescence staining in frozen sections. Cardiac samples of AMPKα2

-/-

mice were used to detect the LC3-Ⅱ/LC3-Ⅰ ratio by Western blotting.

Results

① In WT mice

the levels of red blood cells and hemoglobins in the hypoxic group were increased significantly than those in the normoxic group [(9.33±0.15)×10

(12.78±0.66)×10

0.05; 135±3

192±4 g/L

0.05

]

. ② In WT mice

the ratio of LC3-Ⅱ/LC3-Ⅰwas increased significantly (0.49±0.29

1.70±0.24

0.05)

the p62 expression level was decreased obviously

the relativ

e brightness of p62 was reduced (1.32±0.57

0.71±0.19

0.05)

and the fluorescence intensity of LC3 in the myocardium was increased in the hypoxic group than the normoxic group. ③ In normoxic conditions

the LC3-Ⅱ/LC3-Ⅰ ratio was lower in the myocardium

but not significantly (0.78±0.08

0.73±0.34

0.05) in AMPKα2

-/-

mice than the WT mice. While in hypoxic conditions

the ratio was significantly lower in the myocardium of AMPKα2

-/-

mice than the WT mice (2.08±0.72

1.01±0.21

0.05). ④ In WT mice

the expression level of AMPKα2 proteins was not significantly increased in the hypoxic group than the normoxic group (1.14±0.12

1.25±0.19

0.05).

Conclusion

Chronic hypoxia may enhance autophagy in cardiomyocytes by AMPKα2-dependent pathway

and the autophagy may be the adaptation mechanism of cardiomyocytes to chronic hypoxic conditions.

关键词

Keywords

references

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