Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study

Hongbin Zhu; Wei Shen; Jing Wang; Shuguang Lu; Yan Zhao; Shuai Le; Mengyu Shen; Gang Li; Yuhui Yang; Yali Gong; Ming Li; Yinling Tan; Fuquan Hu

doi:10.16016/j.1000-5404.201611223

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Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study

更新时间：2026-01-09

- Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study
- Journal of Third Military Medical University Vol. 39, Issue 8, Pages: 773-780(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201611223
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- Published：2017-04
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Hongbin Zhu, Wei Shen, Jing Wang, et al. Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study[J]. Journal of Third Military Medical University, 2017, 39(8): 773-780.
DOI：

Hongbin Zhu, Wei Shen, Jing Wang, et al. Correlation of gut microbiota with susceptibility to high-fat diet-induced obesity in mice: a metagenomic study[J]. Journal of Third Military Medical University, 2017, 39(8): 773-780. DOI： 10.16016/j.1000-5404.201611223.

摘要

Abstract

Objective

To investigate the correlation between gut microbiota and the susceptibility to high-fat diet-induced obesity in mice.

Methods

Stool samples were collected from C57BL/6J mice with high-fat diet-induced obesity (HFDIO)

mice with resistance to HFDIO (HFDIOR)

and normal control mice fed with normal diet. High-quality DNA samples extracted from the stool samples in the 3 groups (

=6) were taken for metagenomic sequencing using Hiseq2000 (Illumina Co. Ltd.).

Results

Compared with the control group

the mice with HFDIO and HFDIOR showed significantly increased

Firmicutes

Proteobacteria

and

Deferribacteres

while obviously decreased

Bacteroides

in the stool samples (

0.05). Significant differences were also found in the species of bacteria between the HFDIO and HFDIOR groups. The dominant species in the microbiota compositi

on was

Lachnospiraceae bacterium

10_1 in HFDIO group

and

Lachnospiraceae bacterium

28_4 was the dominant species in HFDIOR group (

0.05). The functionality and metabolism of the gut microbiota differed between the 2 model groups in terms of carbohydrate metabolism

nucleotide metabolism and nucleic acid binding

membrane transport and transferase activity.

Conclusion

The composition and function of the gut microbiota may influence the susceptibility of an individual to HFDIO and HFDIOR

which is helpful in assessing the role of gut microbiota in the occurrence of obesity.

关键词

Keywords

references

Serino M , Nicolas S , Trabelsi M S , et al . Young microbes for adult obesity [J ] . Pediatr Obes , 2016 [Epub ahead of print] . DOI: 10.1111/ijpo.12146. Serino M, Nicolas S, Trabelsi M S, et al. Young microbes for adult obesity[J]. Pediatr Obes, 2016 [Epub ahead of print]. DOI:10.1111/ijpo.12146.

Levin B E Triscari J Hogan S Resistance to diet-induced obesity: food intake, pancreatic sympathetic tone, and insulin Am J Physiol 1987 252 3 Pt 2 R471 R478 http://ajpregu.physiology.org/content/ajpregu/252/3/R471.full.pdf Levin B E, Triscari J, Hogan S, et al. Resistance to diet-induced obesity: food intake, pancreatic sympathetic tone, and insulin[J]. Am J Physiol, 1987, 252(3 Pt 2): R471-R478.

王欢李宛真汪弋力高脂饮食诱导的肥胖及肥胖抵抗小鼠肠道菌群元基因组的比较研究西安交通大学学报 (医学版) 2014 2 240 244 10.7652/jdyxb201402021 王欢, 李宛真, 汪弋力, 等.高脂饮食诱导的肥胖及肥胖抵抗小鼠肠道菌群元基因组的比较研究[J].西安交通大学学报 (医学版). 2014, 2:240-244.DOI:10.7652/jdyxb 201402021.

Wang H Li W Z Wang Y L Comparison of the gut microbiome metagenome in diet-induced obesity mice and diet-induced obesity resistant mice Journal of Xi'an Jiaotong University (Medical Sciences) 2014 2 240 244 10.7652/jdyxb201402021 Wang H, Li W Z, Wang Y L, et al. Comparison of the gut microbiome metagenome in diet-induced obesity mice and diet-induced obesity resistant mice[J]. Journal of Xi'an Jiaotong University (Medical Sciences). 2014, 2: 240-244. DOI:10.7652/jdyxb201402021.

David L A Maurice C F Carmody R N Diet rapidly and reproducibly alters the human gut microbiome Nature 2014 505 7484 559 563 10.1038/nature12820 David L A, Maurice C F, Carmody R N, et al. Diet rapidly and reproducibly alters the human gut microbiome[J]. Nature, 2014, 505(7484): 559-563.DOI:10.1038/nature12820.

McAllan L Skuse P Cotter P D Protein quality and the protein to carbohydrate ratio within a high fat diet influences energy balance and the gut microbiota in C57BL/6J mice PLoS ONE 2014 9 2 e88904 10.1371/journal.pone.0088904 McAllan L, Skuse P, Cotter P D, et al. Protein quality and the protein to carbohydrate ratio within a high fat diet influences energy balance and the gut microbiota in C57BL/6J mice[J]. PLoS ONE, 2014, 9(2): e88904. DOI:10.1371/journal.pone.0088904.

Boi S K Buchta C M Pearson N A Obesity alters immune and metabolic profiles: New insight from obese-resistant mice on high-fat diet Obesity (Silver Spring) 2016 24 10 2140 2149 10.1002/oby.21620 Boi S K, Buchta C M, Pearson N A, et al. Obesity alters immune and metabolic profiles: New insight from obese-resistant mice on high-fat diet[J]. Obesity (Silver Spring), 2016, 24(10): 2140-2149. DOI:10.1002/oby.21620.

Holm J B Rnnevik A Tastesen H S Diet-induced obesity, energy metabolism and gut microbiota in C57BL/6J mice fed Western diets based on lean seafood or lean meat mixtures J Nutr Biochem 2016 31 127 136 10.1016/j.jnutbio.2015.12.017 Holm J B, Rnnevik A, Tastesen H S, et al. Diet-induced obesity, energy metabolism and gut microbiota in C57BL/6J mice fed Western diets based on lean seafood or lean meat mixtures[J]. J Nutr Biochem, 2016, 31: 127-136. DOI:10. 1016/j.jnutbio.2015.12.017.

Bardova K Horakova O Janovska P Early differences in metabolic flexibility between obesity-resistant and obesity-prone mice Biochimie 2016 124 163 170 10.1016/j.biochi.2015.11.014 Bardova K, Horakova O, Janovska P, et al. Early differences in metabolic flexibility between obesity-resistant and obesity-prone mice[J]. Biochimie, 2016, 124: 163-170. DOI:10.1016/j.biochi.2015.11.014.

Xia S F Duan X M Hao L Y Role of thyroid hormone homeostasis in obesity-prone and obesity-resistant mice fed a high-fat diet Metab Clin Exp 2015 64 5 566 579 10.1016/j.metabol.2014.12.010 Xia S F, Duan X M, Hao L Y, et al. Role of thyroid hormone homeostasis in obesity-prone and obesity-resistant mice fed a high-fat diet[J]. Metab Clin Exp, 2015, 64(5): 566-579. DOI:10.1016/j.metabol.2014.12.010.

Riva A Borgo F Lassandro C Pediatric obesity is associated with an altered gut microbiota and discordant shifts in Firmicutes populations Environmental Microbiology 2016 10.1111/1462-2920.13463 Riva A, Borgo F, Lassandro C, et al. Pediatric obesity is associated with an altered gut microbiota and discordant shifts in Firmicutes populations[J]. Environmental Microbiology, 2016. DOI:10.1111/1462-2920.13463.

Walker A Pfitzner B Neschen S Distinct signatures of host-microbial meta-metabolome and gut microbiome in two C57BL/6 strains under high-fat diet ISME J 2014 8 12 2380 2396 10.1038/ismej.2014.79 Walker A, Pfitzner B, Neschen S, et al. Distinct signatures of host-microbial meta-metabolome and gut microbiome in two C57BL/6 strains under high-fat diet[J]. ISME J, 2014, 8(12): 2380-2396. DOI:10.1038/ismej.2014.79.

Kim M S Bae J W Spatial disturbances in altered mucosal and luminal gut viromes of diet-induced obese mice Environ Microbiol 2016 18 5 1498 1510 10.1111/1462-2920.13182 Kim M S, Bae J W. Spatial disturbances in altered mucosal and luminal gut viromes of diet-induced obese mice[J]. Environ Microbiol, 2016, 18(5): 1498-1510. DOI:10.1111/1462-2920.13182.

Zhong Y Nyman M Fāk F Modulation of gut microbiota in rats fed high-fat diets by processing whole-grain barley to barley malt Mol Nutr Food Res 2015 59 10 2066 2076 10.1002/mnfr.201500187 Zhong Y, Nyman M, Fāk F. Modulation of gut microbiota in rats fed high-fat diets by processing whole-grain barley to barley malt[J]. Mol Nutr Food Res, 2015, 59(10): 2066-2076. DOI:10.1002/mnfr.201500187.

Zeng H Ishaq S L Zhao F Colonic inflammation accompanies an increase of β-catenin signaling and Lachnospiraceae/Streptococcaceae bacteria in the hind gut of high-fat diet-fed mice J Nutr Biochem 2016 35 30 36 10.1016/j.jnutbio.2016.05.015 Zeng H, Ishaq S L, Zhao F, et al. Colonic inflammation accompanies an increase of β-catenin signaling and Lachnospiraceae/Streptococcaceae bacteria in the hind gut of high-fat diet-fed mice[J]. J Nutr Biochem, 2016, 35: 30-36. DOI:10.1016/j.jnutbio.2016.05.015.

Meehan C J Beiko R G A phylogenomic view of ecological specialization in the Lachnospiraceae, a family of digestive tract-associated bacteria Genome Biol Evol 2014 6 3 703 713 10.1093/gbe/evu050 Meehan C J, Beiko R G. A phylogenomic view of ecological specialization in the Lachnospiraceae, a family of digestive tract-associated bacteria[J]. Genome Biol Evol, 2014, 6(3): 703-713. DOI:10.1093/gbe/evu050.

Kameyama K Itoh K Intestinal colonization by a Lachnospiraceae bacterium contributes to the development of diabetes in obese mice Microbes Environ 2014 29 4 427 430 10.1264/jsme2.ME14054 Kameyama K, Itoh K. Intestinal colonization by a Lachnospiraceae bacterium contributes to the development of diabetes in obese mice[J]. Microbes Environ, 2014, 29(4): 427-430. DOI:10.1264/jsme2.ME14054.

Daniel H Gholami A M Berry D High-fat diet alters gut microbiota physiology in mice ISME J 2014 8 2 295 308 10.1038/ismej.2013.155 Daniel H, Gholami A M, Berry D, et al. High-fat diet alters gut microbiota physiology in mice[J]. ISME J, 2014, 8(2): 295-308. DOI:10.1038/ismej.2013.155.

Salonen A Lahti L Saloj rvi J Impact of diet and individual variation on intestinal microbiota composition and fermentation products in obese men ISME J 2014 8 11 2218 2230 10.1038/ismej.2014.63 Salonen A, Lahti L, Saloj rvi J, et al. Impact of diet and individual variation on intestinal microbiota composition and fermentation products in obese men[J]. ISME J, 2014, 8(11): 2218-2230. DOI:10.1038/ismej.2014.63.

Everard A Lazarevic V Derrien M Responses of gut microbiota and glucose and lipid metabolism to prebiotics in genetic obese and diet-induced leptin-resistant mice Diabetes 2011 60 11 2775 2786 10.2337/db11-0227 Everard A, Lazarevic V, Derrien M, et al. Responses of gut microbiota and glucose and lipid metabolism to prebiotics in genetic obese and diet-induced leptin-resistant mice[J]. Diabetes, 2011, 60(11): 2775-2786. DOI:10.2337/db11-0227.

Arumugam M Raes J Pelletier E Enterotypes of the human gut microbiome Nature 2011 473 7346 174 180 10.1038/nature09944 Arumugam M, Raes J, Pelletier E, et al. Enterotypes of the human gut microbiome[J]. Nature, 2011, 473(7346): 174-180. DOI:10.1038/nature09944.

Xu W T Nie Y Z Yang Z The crosstalk between gut microbiota and obesity and related metabolic disorders Future Microbiol 2016 11 825 836 10.2217/fmb-2015-0024 Xu W T, Nie Y Z, Yang Z, et al. The crosstalk between gut microbiota and obesity and related metabolic disorders[J]. Future Microbiol, 2016, 11: 825-836. DOI:10.2217/fmb-2015-0024.

Sze M A Schloss P D Looking for a Signal in the Noise: Revisiting Obesity and the Microbiome mBio 2016 7 4 e01018 e01016 10.1128/mbio.01018-16 Sze M A, Schloss P D. Looking for a Signal in the Noise: Revisiting Obesity and the Microbiome[J]. mBio, 2016, 7(4): e01018-e01016.DOI: 10.1128/mbio.01018-16.

Arora T Bckhed F The gut microbiota and metabolic disease: current understanding and future perspectives J Intern Med 2016 280 4 339 349 10.1111/joim.12508 Arora T, Bckhed F. The gut microbiota and metabolic disease: current understanding and future perspectives[J]. J Intern Med, 2016, 280(4): 339-349.DOI:10.1111/joim.12508.

Han E Kim G Hong N Association between dietary acid load and the risk of cardiovascular disease: nationwide surveys (KNHANES 2008-2011) Cardiovasc Diabetol 2016 15 1 10.1186/s12933-016-0436-z Han E, Kim G, Hong N, et al. Association between dietary acid load and the risk of cardiovascular disease: nationwide surveys (KNHANES 2008-2011)[J]. Cardiovasc Diabetol, 2016, 15(1). DOI: 10.1186/s12933-016-0436-z.

Fam B C Sgambellone R Ruan Z Contribution of the hypothalamus and gut to weight gain susceptibility and resistance in mice J Endocrinol 2015 225 3 191 204 10.1530/JOE-15-0131 Fam B C, Sgambellone R, Ruan Z, et al. Contribution of the hypothalamus and gut to weight gain susceptibility and resistance in mice[J]. J Endocrinol, 2015, 225(3): 191-204. DOI: 10.1530/JOE-15-0131.

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