Effects of sleep deprivation on antioxidant activity and autophagy in locus coeruleus neurons in mice

Nana Ni; Shuancheng Ren; Li Yang; Quanhui Chen; Zhian Hu; Shuangyi Fan

doi:10.16016/j.1000-5404.201611219

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Effects of sleep deprivation on antioxidant activity and autophagy in locus coeruleus neurons in mice

更新时间：2026-01-09

- Effects of sleep deprivation on antioxidant activity and autophagy in locus coeruleus neurons in mice
- Journal of Third Military Medical University Vol. 39, Issue 8, Pages: 743-748(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Innovation Research Fund of Affiliated Hospital of Academy of Military Medical Sciences(FC-2012-18)
- DOI：10.16016/j.1000-5404.201611219
  CLC：
- Received：15 November 2016，
  
  Published：30 April 2017
- 稿件说明：
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Nana Ni, Shuancheng Ren, Li Yang, et al. Effects of sleep deprivation on antioxidant activity and autophagy in locus coeruleus neurons in mice[J]. Journal of Third Military Medical University, 2017, 39(8): 743-748.
DOI：

Nana Ni, Shuancheng Ren, Li Yang, et al. Effects of sleep deprivation on antioxidant activity and autophagy in locus coeruleus neurons in mice[J]. Journal of Third Military Medical University, 2017, 39(8): 743-748. DOI： 10.16016/j.1000-5404.201611219.

摘要

Abstract

Objective

To observe the effects of sleep deprivation on the antioxidant activity and autophagy in the neurons in the locus coeruleus of mice.

Methods

Fifty-four adult male C57BL/6 mice (8-12 weeks old

weighing 23-26 g) were randomized into total sleep deprivation (induce

d by gentle stimulation) for 6 h group (

=8)

total sleep deprivation for 12 h group (

=8)

and rapid eye movement sleep deprivation (RSD; induced using a modified multiple platform method) group (

=11)

and each group was assigned a normal control group with the same number of mice. Western blotting was used to detect the expression levels of superoxide dismutase 2 (SOD2)

catalase

microtubule-associated protein 1 light chain 3 Ⅱ (LC3-Ⅱ) and P62 (also known as SQSTM1)

and immunofluorescence staining was used to detect the expression of lysosomal-associated membrane protein 1 (LAMP-1) in the locus coeruleus and the lateral hypothalamus of the mice.

Results

The protein levels of SOD2 and catalase were significantly increased in the neurons of the locus coeruleus in the mice after sleep deprivation for 6 h (

0.05)

but showed no further increment after sleep deprivation for 12 h. RSD significantly decreased the protein levels of SOD2 and catalase in the locus coeruleus neurons (

0.05). In the lateral hypothalamic neurons

sleep deprivation resulted in no significant changes in the expression levels of catalase or SOD2. Sleep deprivation for 6 and 12 h did not significantly affect the expression of LC3-Ⅱ and P62 in the locus coeruleus

whereas RSD caused a significant decrease in LC3-Ⅱ expression (

0.05)

increased P62 level (

0.05) and reduced LAMP-1 level in the locus coeruleus neurons (

0.05). Sleep deprivation did not affect the expression levels of LC3-Ⅱ or P62 in the lateral hypothalamic neurons.

Conclusion

Short-term total sleep deprivation causes a transient increase in the antioxidant activity whereas has no significant effect on autophagy in the locus coeruleus neurons. Long-term RSD significantly reduces both the antioxidant activity and autophagy in the locus coeruleus neurons of mice.

关键词

Keywords

references

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