Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression <italic style="font-style: italic">via</italic> PI3K/Akt signal pathway

Dapeng Yang; Hongbo Huan; Liang Zhang; Xudong Wen; Lili Wu; Feng Xia

doi:10.16016/j.1000-5404.201611189

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Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression via PI3K/Akt signal pathway

更新时间：2026-01-09

- Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression via PI3K/Akt signal pathway
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 821-826(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81272224)
- DOI：10.16016/j.1000-5404.201611189
  CLC：
- Received：22 November 2016，
  
  Published：15 May 2017
- 稿件说明：
移动端阅览
Dapeng Yang, Hongbo Huan, Liang Zhang, et al. Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression via PI3K/Akt signal pathway[J]. Journal of Third Military Medical University, 2017, 39(9): 821-826.
DOI：

Dapeng Yang, Hongbo Huan, Liang Zhang, et al. Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression via PI3K/Akt signal pathway[J]. Journal of Third Military Medical University, 2017, 39(9): 821-826. DOI： 10.16016/j.1000-5404.201611189.

摘要

Abstract

Objective

To determine the effect of norepinephrine on self-renewal capacity in hepatocellular carcinoma (HCC) cells and investigate the molecular mechanism.

Methods

Quantitative RT-PCR and Western blotting were used to detect the expression level of CD133 in the HCC cell lines Huh7 and PLC after the treatment of 10 μmol/L norepinephrine. Sphere and clone formation assays were used to investigate the impact of norepinephrine on self-renewal capacity of HCC cells. The effects of norepinephrine (10 μmol/L) and prazosin (5 μmol/L) on the expression of stem related genes and signal pathways were detected by RT-PCR and Western blotting.

Results

Norepinephrine promoted the expression of CD133 in Huh7 and PLC cells. Norepinephrine facilitated sphere and clone formation in the 2 cell lines

and prazosin blocked the effect of norepinephrine (

0.05). There

were more sphere and clone formation in the cells after norepinephrine treatment than control cells and prazosin group (

0.05). Higher level of Sox4 and up-regulation of p-Akt were observed in the norepinephrine-treated cells than prazosin-treated and control HCC cells (

0.05).

Conclusion

Norepinephrine activates α-adrenergic receptor and up-regulates Sox4 expression

via

activating PI3K/Akt signal pathway

and thus improves self-renewal capacity in HCC cells.

关键词

Keywords

references

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Norepinephrine promotes proliferation of hepatocellular carcinoma cells through enhancing Sox4 expression via PI3K/Akt signal pathway

DOI：10.16016/j.1000-5404.201611189

摘要

Abstract

关键词

Keywords

references