Expression and significance of chemokine MCP-1 and its receptor CCR2 in patients with co-existing papillary thyroid cancer and Hashimoto's thyroiditis

Rong Zeng; Heng Niu; Guoqiao Zhang; Kai Wang; Xinmin Yan

doi:10.16016/j.1000-5404.201611184

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Expression and significance of chemokine MCP-1 and its receptor CCR2 in patients with co-existing papillary thyroid cancer and Hashimoto's thyroiditis

更新时间：2026-01-09

- Expression and significance of chemokine MCP-1 and its receptor CCR2 in patients with co-existing papillary thyroid cancer and Hashimoto's thyroiditis
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 660-667(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Research Project of Medical and Health Institutions of Yunnan Province(2014N269)
- DOI：10.16016/j.1000-5404.201611184
  CLC：
- Received：21 November 2016，
  
  Published：2017-07
- 稿件说明：
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Rong Zeng, Heng Niu, Guoqiao Zhang, et al. Expression and significance of chemokine MCP-1 and its receptor CCR2 in patients with co-existing papillary thyroid cancer and Hashimoto's thyroiditis[J]. Journal of Third Military Medical University, 2017, 39(7): 660-667.
DOI：

Rong Zeng, Heng Niu, Guoqiao Zhang, et al. Expression and significance of chemokine MCP-1 and its receptor CCR2 in patients with co-existing papillary thyroid cancer and Hashimoto's thyroiditis[J]. Journal of Third Military Medical University, 2017, 39(7): 660-667. DOI： 10.16016/j.1000-5404.201611184.

摘要

Abstract

Objective

To investigate the expression of chemokine monocyte chemotactic protein-1 (MCP-1

also known as CCL2)

and its receptor CC chemokine receptor 2 (CCR2) in the patients with co-existing papillary thyroid cancer (PTC) and Hashimoto's thyroiditis (HT)

and to explore the biological effects of MCP-1/CCR2 signaling axis on the occurrence and development of PTC during the progression of HT-PTC.

Methods

Fresh thyroid tissues obtained by surgical removal (including thyroid carcinoma tissues from PTC and PTC-HT patients

and thyroid tissues of HT with pathological confirmation) and peripheral blood serum of above patients. The expression levels of CCR2 and MCP-1 in the thyroid tissues and blood samples were determined by real-time fluorescence quantitative polymerase chain reaction (RT-PCR) and cytometric bead array (CBA). Multi-group differences in genetic screening

co-expression network construction and significant differences in gene function analyses were detected by cDNA microarray in the thyroid tissues from PTC

PTC-HT and HT patients.

Results

① RT-PCR results showed that the mRNA expression levels of CCR2 were significantly higher in the HT thyroid tissue [(10.98±14.49)×10

-3

]

than PTC [(2.63±4.97)×10

-3

]

and PTC-HT carcinoma tissue [(3.77±4.65)×10

-3

Chi

square=3.78

=0.03

]

. The level in the PTC patients was negatively correlated with tumor diameter (

=0.01)

and positively correlated with the levels of thyroglobulin antibody (TGAb) and anti-thyroid perox

idase antibody (TPOAb

=0.03

=0.01). ②CBA results indicated that the serum MCP-1 level was higher in the PTC-HT patients (64.76±96.08 pg/mL) than the other 2 groups

but without statistically significance (

=1.04

=0.36). ③Gene microarray displayed that there was no significant difference in the mRNA level of MCP-1 among the 3 groups (

0.05)

but that of CCR2 was obviously higher in the HT group than the other 2 groups (

=0.014

FDR=0.082). Co-expression network analysis showed the there were 8 genes related to the expressional differences of CCR2

whose functions were mainly involved in the 19 immune-related functions.

Conclusion

MCP-1/CCR2 signaling axis has no synergistic interaction or correlation with PTC occurrence during the progression of HT-PTC. CCR2 may exerts its positive effect through TGAb and TPOAb.

关键词

Keywords

references

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