Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures

Haolong JIN; Zhijin CHEN; Kun XIONG; Xiaolin JIN

doi:10.16016/j.1000-5404.201611159

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Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures

Clinical Medicine | 更新时间：2026-01-09

- Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures
- Journal of Third Military Medical University Vol. 39, Issue 14, Pages: 1481-1485(2017)
- 作者机构：
  
  1.400037 重庆，第三军医大学新桥医院感染控制科
  2.400038 重庆，第三军医大学基础医学部微生物学教研室，重庆市微生物工程实验室
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation(31570173)
- DOI：10.16016/j.1000-5404.201611159
  CLC： R136.32; R446
- Received：18 November 2016，
  
  Revised：2017-02-23，
  
  Published：30 July 2017
- 稿件说明：
移动端阅览
Haolong JIN, Zhijin CHEN, Kun XIONG, et al. Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures[J]. Journal of Third Military Medical University, 2017, 39(14): 1481-1485.
DOI：

Haolong JIN, Zhijin CHEN, Kun XIONG, et al. Total volatile organic compound pollution resulting from Ziehl-Neelsen acid-fast staining in laboratory and its control measures[J]. Journal of Third Military Medical University, 2017, 39(14): 1481-1485. DOI： 10.16016/j.1000-5404.201611159.

摘要

目的

建立一种改良抗酸染色冷染方法，用以控制实验室总挥发性有机物(total volatile organic compound，TVOC)污染。

方法

采用TVOC检测仪定量测定实验室内空气中的苯系物和TVOC污染程度；以卡介苗(bacillecalmette-guerin，BCG)为染色标本，配制含有1% Triton X-100标本稀释液和初染液，建立不用加温助染的抗酸染色冷染法替代传统抗酸染色法。

结果

传统萋-尼抗酸染色时，室内TVOC污染在12 min时间点即高达1 014 ppb(约1 mg/m

)，成倍超过国家室内卫生标准(GB50325-2010) 规定值(500 ppb

即0.5 mg/m

)；同样条件下，用改良冷染法染色期间，室内TVOC量有所增加，但在试验期间最高值为387 ppb

保持在国家室内卫生标准容许限度以下；利用Triton X-100建立的改良抗酸冷染法具有抗酸染色特性，其对抗酸杆菌的可检测度为3×10

-6

~6×10

-5

CFU/mL。

结论

抗酸染色冷染法可用于替代传统萋-尼抗酸染色，从而控制实验室的TVOC污染问题。

Abstract

Objective

To detect total volatile organic compound (TVOC) pollution in the laboratory during Ziehl-Neelsen acid-fast staining and establish an improved method for controlling TVOC pollution.

Methods

TVOC concentration in the room air of the laboratory was determined using RAE portable-type instrument during the performance of routine acid-fast staining of Bacille Calmette-Guerin (BCG). A dilution solution and a carbolfuchsin solution containing 1% Triton X-100 were prepared for use in the modified cold acid-fast staining without heating

and the TVOC pollution level caused by the modified method and the routine acid-fast staining method was compared.

Results

At 12 min after the start of routine acid-fast staining

the concentration of TVOC in the room air of the laboratory reached the highest level of 1 014 ppb (about 1 mg/m

)

which was 2 folds of the permissible exposure limit (500 ppb

0.5 mg/m

) in the National Standard (GB50325-2010). During the performance of the modified cold acid-fast staining

TVOC concentration in the laboratory increased only slightly and remained under the permissible exposure limit. The detection sensitivity of BCG ranged from 3×10

-6

to 6×10

-5

CFU using the modified cold staining method.

Conclusion

The modified acid-fast cold staining method can be used as an alternative to the conventional acid-fast staining for controlling TVOC pollution in the laboratory.

关键词

Keywords

references

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