Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis

Guan Yang; Dongye Wu; Heda Xiao; Hongyin Liang; Hongyu Sun; Lijun Tang

doi:10.16016/j.1000-5404.201611157

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Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis

更新时间：2026-01-09

- Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 872-878(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81500409)
- DOI：10.16016/j.1000-5404.201611157
  CLC：
- Received：17 November 2016，
  
  Published：15 May 2017
- 稿件说明：
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Guan Yang, Dongye Wu, Heda Xiao, et al. Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis[J]. Journal of Third Military Medical University, 2017, 39(9): 872-878.
DOI：

Guan Yang, Dongye Wu, Heda Xiao, et al. Effects of early abdominal paracentesis drainage on intestinal bacterial translocation in rats with severe acute pancreatitis[J]. Journal of Third Military Medical University, 2017, 39(9): 872-878. DOI： 10.16016/j.1000-5404.201611157.

摘要

Abstract

Objective

To determine the effect of abdominal paracentesis drainage (APD) on the intestinal bacterial translocation in rats with severe acute pancreatitis (SAP).

Methods

The rats were intragastrically fed with

Escherichia coli

whose plasmids were reconstructed with green fluorescent protein (GFP-

E.coli

). These GFP-

E.coli

labeled rats were randomly divided into 3 groups: sham operation group (Sham group)

SAP group and APD group. The SAP rats were estab

lished by retrograde infusion of sodium taurocholate into biliopancreatic duct. The APD treatment was applied by inserting a drainage tube into the right lower quadrant of rats after SAP establishment. Spleen specimens were harvested in a sterile manner along with scarifying the rats in 24 h after operation. Bacterial translocation in the blood

mesenteric lymph nodes (MLNs) and pancreatic tissues

level of serum endotoxin

histological scores of pancreas

and serum levels of inflammatory factors CRP

TNF-α and IL-1β were assessed and compared among the 3 groups.

Results

Compared with sham operation group

the histological score of pancreas

bacterial translocation in the blood

MLNs and pancreatic tissues

serum endotoxin level

and serum levels of CRP

TNF-α and IL-1β were all significantly higher in the SAP group and APD group (

0.05). While compared with the SAP group

the above indicators were obviously lower in the APD group (

0.05).

Conclusion

Early APD reduces intestinal bacterial translocation and systemic inflammatory response in rats with SAP

and thus attenuates the severity of SAP.

关键词

Keywords

references

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