Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity

Jian Chen; Hangwei Fu; Menggang Liu; Jingxiang Chen; Jianhua Xu; Yuzhou Wang; Ping Chen

doi:10.16016/j.1000-5404.201611156

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Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity

更新时间：2026-01-09

- Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 827-832(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of the National Natural Science Foundation of China(81270523);the National Natural Science Foundation for Young Scholars of China(81400651)
- DOI：10.16016/j.1000-5404.201611156
  CLC：
- Received：17 November 2016，
  
  Published：15 May 2017
- 稿件说明：
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Jian Chen, Hangwei Fu, Menggang Liu, et al. Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity[J]. Journal of Third Military Medical University, 2017, 39(9): 827-832.
DOI：

Jian Chen, Hangwei Fu, Menggang Liu, et al. Tmub1 silencing promotes hepatocytes proliferation by enhancing Akt activity[J]. Journal of Third Military Medical University, 2017, 39(9): 827-832. DOI： 10.16016/j.1000-5404.201611156.

摘要

Abstract

Objective

To determine the effect of Tmub1 silencing on the phosphorylation of Akt and the proliferation and growth of hepatocytes.

Methods

RNA interference technique was used to knockdown Tmub1 gene in rat liver cell line BRL-3A. qRT-PCR and Western blotting were used to detect the expression of Tmub1

Akt

p-Akt

Cyclin D1 and c-myc at mRNA and protein levels. Plate colony formation assay and CCK-8 assay were employed to measure the proliferation and growth of BRL-3A cells. Flow cytometry was used to determine the cell cycle.

Results

Compared t

o the control group

the mRNA levels of Cyclin D1 and c-myc were up-regulated (

0.05)

the protein levels of p-Akt

Cyclin D1 and c-myc were increased (

0.05)

the proliferation and growth of BRL-3A cells were enhanced (

0.05)

and the percentage of cells at S+G

/M period was significantly increased (

0.05) in the cells after Tmub1 silencing. When the phosphorylation of Akt was inhibited by MK2206

the mRNA and protein levels of the genes mentioned above were significantly down-regulated

the proliferation ability of the BRL-3A cells was obviously reduced (

0.05)

and the percentage of cells at S+G

/M period was notably deceased (

0.05).

Conclusion

Tmub1 silencing promotes the proliferation and growth of BRL-3A cells by enhancing the phosphorylation of Akt and increasing the expression levels of Cyclin D1 and c-myc.

关键词

Keywords

references

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