Dynamic pathological changes of myocardial fibrosis in mice in an early stage of ventricular pressure overload

Yongsheng Meng; Zhao Jian; Yun Zhu; Chao Liu; Yunhan Jiang; Guiping Luo; Yu Zhu; Fuqin Tang; Yingbin Xiao

doi:10.16016/j.1000-5404.201611150

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Dynamic pathological changes of myocardial fibrosis in mice in an early stage of ventricular pressure overload

更新时间：2026-02-09

- Dynamic pathological changes of myocardial fibrosis in mice in an early stage of ventricular pressure overload
- Journal of Third Military Medical University Vol. 39, Issue 10, Pages: 966-970(2017)
- 作者机构：
  
  400037 重庆，第三军医大学新桥医院全军心血管外科研究所
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201611150
  CLC： R322.11;R541.02;R542.23
- Received：15 November 2016，
  
  Revised：2017-01-31，
  
  Published：30 May 2017
- 稿件说明：
移动端阅览
Yongsheng Meng, Zhao Jian, Yun Zhu, et al. Dynamic pathological changes of myocardial fibrosis in mice in an early stage of ventricular pressure overload[J]. Journal of Third Military Medical University, 2017, 39(10): 966-970.
DOI：

Yongsheng Meng, Zhao Jian, Yun Zhu, et al. Dynamic pathological changes of myocardial fibrosis in mice in an early stage of ventricular pressure overload[J]. Journal of Third Military Medical University, 2017, 39(10): 966-970. DOI： 10.16016/j.1000-5404.201611150.

摘要

目的

通过主动脉弓缩窄建立小鼠心室压力超负荷模型，探讨在心室压力超负荷早期心肌纤维化的动态病理学变化。

方法

选用10周龄、体质量23 ~ 25 g的雄性C57BL/6J小鼠40只，随机分为4组（

＝ 10）：① 假手术组（SHAM组）、② 缩窄3 d组（TAC3D组）、③ 缩窄7 d组（TAC7D组）、④ 缩窄14 d组（TAC14D组）。各缩窄组小鼠行主动脉弓缩窄术，假手术组除不缩窄主动脉弓部，其他操作同各缩窄组。心脏导管检测小鼠心脏功能变化情况；采用心脏体质量比、心肌细胞直径大小等指标评价小鼠心肌结构代偿性变化情况；Masson染色观察小鼠心肌纤维化变化情况。

结果

① 与假手术组相比，各缩窄组射血分数无显著改变（

＞0.05）；② 与假手术组相比，各缩窄组心体比显著增加，心肌细胞直径显著增大[SHAM组、TAC3D组、TAC7D组、TAC14D组心肌细胞直径依次为（13.56 ± 0. 24）、（18.02 ± 0.52）、（18.76 ± 0.96）、（21.20 ± 1.85）μm，

＜0.05

]

。③ 与假手术组相比，各缩窄组心肌管周纤维化程度显著增加，且随缩窄时间延长而加重[ SHAM组、TAC3D组、TAC7D组、TAC14D组血管周围纤维化面积与血管面积比依次为（0.18 ± 0.02）、（0.37 ± 0.02）、（1.22 ± 0.08）、（2.05 ± 0.12），

＜0.05

]

。

结论

在心室压力超负荷早期，心肌纤维化主要出现于血管周围，且纤维化程度随缩窄时间延长而逐渐加重。

Abstract

Objective

To investigate the dynamic pathological changes of myocardial fibrosis in an early stage of ventricular pressure overload in a mouse model of transverse aortic constriction (TAC).

Methods

Forty male C57BL/6J mice were randomly divided into the sham-operated group and 3 TAC groups for observation at 3

and 14 d after TAC (TAC3D

TAC7D and TAC14D groups

respectively). Cardiac function of the mice was examined with cardiac catheter measurement. The myocardial structure changes were evaluated based on the heart weight index and the myocardial cell diameter

and the changes in the myocardial fibers were observed with the Masson staining.

Results

Compared with those in the sham-operated group

the mice in the TAC groups showed no significant changes in the ejection fraction (

0.05)

but the heart-to-body weight ratio and myocyte width were increased obviously (the myocyte width in sham

TAC3D

TAC7D and TAC14D groups was 13.56 ± 0.24

18.02 ± 0.52

18.76 ± 0.96

and 21.20 ± 1.85 μm

respectively

0.05). Compared with that in the sham-operated group

the severity of perivascular fibrosis increased significantly in the TAC groups and exacerbated as the TAC time extended (the perivascular fibrosis area/vascular area ratios in the 4 groups were 0.18 ± 0.02

0.37 ± 0.02

1.22 ± 0. 08

and 2.05 ± 0.12

respectively

0.05).

Conclusion

Perivascular fibrosis is the major pathological finding in the early stage of ventricular pressure overload in mice and deteriorates progressively with the extension of TAC time.

关键词

Keywords

references

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