Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells

Zhiping Zhao; Jian Li; Yunchao Wang; Huan Qin; Weiwu Gao; Chenhui Wang; Huaizhi Wang

doi:10.16016/j.1000-5404.201611074

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Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells

更新时间：2026-01-09

- Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells
- Journal of Third Military Medical University Vol. 39, Issue 9, Pages: 840-845(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81372242);the Clinical Research Foundation of Third Military Medical University(SWH2015LC01)
- DOI：10.16016/j.1000-5404.201611074
  CLC：
- Received：09 November 2016，
  
  Published：15 May 2017
- 稿件说明：
移动端阅览
Zhiping Zhao, Jian Li, Yunchao Wang, et al. Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells[J]. Journal of Third Military Medical University, 2017, 39(9): 840-845.
DOI：

Zhiping Zhao, Jian Li, Yunchao Wang, et al. Effect of LncRNA LOC100506123 on proliferation and migration in pancreatic cancer cells[J]. Journal of Third Military Medical University, 2017, 39(9): 840-845. DOI： 10.16016/j.1000-5404.201611074.

摘要

Abstract

Objective

To determine the effect of long non-coding RNA (LncRNA) LOC100506123 on the proliferation and migration of pancreatic cancer cells.

Methods

Quantitative real-time PCR (qRT-PCR) was used to detect the expression of LOC100506123 in pancreatic cell lines

including normal pancreatic duct epithelial cell line HPDE and 4 pancreatic cancer cell lines

AsPC-1

BxPC-3

Panc-1 and CFPAC-1

and pancreatic cancer tissues. Small interfering RNA (siRNA) targeting LOC100506123 by RNA interference technique was constructed and then transfected into AsPC-1 and CFPAC-1 cells with Lipofectamine 2000 (including the control group siR-NC and the experimental groups siR-1 and siR-2). The proliferation and migration of pancreatic cancer cells were detected by Cell Counting Kit-8 (CCK-8) assay and Transwell chamber test

respectively.

Results

① LncRNA LOC100506123 was over-expressed in the pancreatic cancer cells and tissues (

0.05). ② Down-regulation of LOC100506123 significantly inhibited the proliferation and migration of pancreatic cancer cells (

0.05).

Conclusion

Over-expressed LOC100506123 promotes the proliferation and migration in pancreatic cancer cells.

关键词

Keywords

references

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