Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy

Junjie Yuan; Kehong Chen; Lirong Lin; Luquan Zheng; Yani He

doi:10.16016/j.1000-5404.201611059

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Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy

更新时间：2026-01-09

- Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy
- J Third Mil Med Univ Vol. 39, Issue 4, Pages: 349-354(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81670661，81400733，81470962)
- DOI：10.16016/j.1000-5404.201611059
  CLC：
- Received：07 November 2016，
  
  Published：28 February 2017
- 稿件说明：
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Junjie Yuan, Kehong Chen, Lirong Lin, et al. Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy[J]. J Third Mil Med Univ, 2017, 39(4): 349-354.
DOI：

Junjie Yuan, Kehong Chen, Lirong Lin, et al. Relationship between E3 ubiquitin ligase Parkin expression and renal tubulointerstitial injury in diabetic nephropathy[J]. J Third Mil Med Univ, 2017, 39(4): 349-354. DOI： 10.16016/j.1000-5404.201611059.

摘要

Abstract

Objective

To investigate the expression of Parkin in renal tissues of patients with diabetic nephropathy (DN) and the relationship between Parkin expression and renal tubulointerstitial injury.

Method

Totally 40 patients diagnosed with type 2 DN by renal biopsy admitted in our hospital between 2015 and 2016 were enrolled in this study. Ten patients with pathologically confirmed normal kidney tissues after renal tumor resection were taken as control group. Immunohistochemical examination were performed to detect the Parkin expression in renal tissues with different tubulointerstitial injury scored by interstitial fibrosis and tubular atrophy (IFTA) classification. The expression of Parkin correlating to clinical factors and renal pathological damage was determined by Pearson and Spearman correlation analyses

respectively. Immunofluorescence staining was performed to determine the expression of Parkin

renal interstitial injury factors e.g. interleukin (IL)-6

transforming growth factor beta (TGF-β)

and interstitial fibrosis marker collagen Ⅳ (Col-Ⅳ).

Results

Parkin was mainly expressed in the cytoplasm of renal tubular epithelial cells

and its expression level was gradually decreased with increased IFTA score. There was a significant negative correlation between Parki

n expression and renal tissue injury scores (glomerulosclerosis

tubular atrophy and interstitial fibrosis

renal interstitial inflammation) (

0.01). Such negative correlation was also found between Parkin expression and renal function injury parameters (quantitative urinary protein

urinary N-Acetyl-β-D glucosaminidase

blood urea nitrogen

serum cystatin C

serum creatinine)

while Parkin expression was positively correlated with estimated glomerular filtration rate (eGFR) (

0.05). Immunofluorescence assay showed that renal tubular epithelial cells with positive expression of Parkin did not co-express IL-6

TGF-β and Col-IV.

Conclusion

Decreased expression of Parkin is closely related to renal tubulointerstitial injury in patients with DN.

关键词

Keywords

references

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