Effects of doxorubicin on cofilin interacting proteins in nucleus of breast cancer cells through comparative proteomics

Yanxia Liu; Yunong Li; Ruoqiu Fu; Ning Gao

doi:10.16016/j.1000-5404.201611024

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Effects of doxorubicin on cofilin interacting proteins in nucleus of breast cancer cells through comparative proteomics

更新时间：2026-01-09

- Effects of doxorubicin on cofilin interacting proteins in nucleus of breast cancer cells through comparative proteomics
- Journal of Third Military Medical University Vol. 39, Issue 6, Pages: 522-528(2017)
- 作者机构：
  
  400038 重庆，第三军医大学药学系生药学与天然药物化学教研室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(31571425)
- DOI：10.16016/j.1000-5404.201611024
  CLC： R737.9;R966;R979.1
- Received：03 November 2016，
  
  Revised：2016-12-18，
  
  Published：2017-06
- 稿件说明：
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Yanxia Liu, Yunong Li, Ruoqiu Fu, et al. Effects of doxorubicin on cofilin interacting proteins in nucleus of breast cancer cells through comparative proteomics[J]. Journal of Third Military Medical University, 2017, 39(6): 522-528.
DOI：

Yanxia Liu, Yunong Li, Ruoqiu Fu, et al. Effects of doxorubicin on cofilin interacting proteins in nucleus of breast cancer cells through comparative proteomics[J]. Journal of Third Military Medical University, 2017, 39(6): 522-528. DOI： 10.16016/j.1000-5404.201611024.

摘要

目的

采用比较蛋白质组学的方法，检测多柔比星对乳腺癌细胞核内丝切蛋白 (cofilin) 相互作用蛋白的影响。

方法

采用Western blot检测多柔比星对细胞核内cofilin表达的影响；免疫共沉淀后，采用比较蛋白质组学方法，检测对照组与多柔比星处理组细胞核内cofilin相互作用蛋白的差异；采用流式细胞仪检测多柔比星对细胞周期的影响。

结果

多柔比星处理MDA-MB-231细胞后，细胞核内cofilin表达增加，呈明显量效和时效关系；与对照组比较，多柔比星处理组核内与cofilin相互作用的蛋白结合蛋白、结构分子激活蛋白、细胞骨架蛋白、核酸结合蛋白、核糖体蛋白的种类明显减少 (109、60、45、76、34

79、32、20、56、16)；多柔比星处理细胞后，可引起细胞周期阻滞在S/G

期，并呈明显量效关系，G

期细胞由 (45.92±4.57)%降至 (10.50±0.83)% (

0.01)。

结论

多柔比星处理细胞后可增加cofilin核聚集，减少细胞核内与cofilin相互作用蛋白的种类，最终使细胞周期阻滞在S/G

期。

Abstract

Objective

To determine the effects of doxorubicin on cofilin interacting proteins in the nucleus of breast cancer cells.

Methods

The expression of cofilin in the nucleus was evaluated by Western blotting. After immunoprecipitation

the differences of cofilin interacting proteins in the nucleus between the controls and doxorubicin-treated cells were determined by comparative proteomics. The effects of doxorubicin on cell cycle were determined by flow cytometry.

Results

Doxorubicin treatment resulted in an increase of cofilin level in the nucleus of MDA-MB-231 cells in dose-and time-dependent manner. Compared with the controls

doxorubicin treatment also resulted in a decreases of the types of cofilin interacting proteins including protein binding proteins

structural molecule activity proteins

cytos

keleton proteins

nucleic acid binding proteins

and ribosome proteins (109

and 34

and 16

respectively). Furthermore

doxorubicin treatment led to cell cycle arrest at S/G

phase in a dose-dependent manner and the percentage of cells at G

phase was declined from (45.92±4.57)% to (10.50±0.83)% (

0.01).

Conclusion

Doxorubicin treatment increases the accumulation of cofilin in the nuclear

decreases the types of cofilin interacting proteins

and finally leads to cell cycle arrest at S/G2 phase.

关键词

Keywords

references

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