Over-expression of endothelin 3 suppresses migration and invasion in colorectal cancer LoVo cells

Shili Xiang; Qian Xiao; Tingxiu Xiang; Tao Luo

doi:10.16016/j.1000-5404.201610226

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Over-expression of endothelin 3 suppresses migration and invasion in colorectal cancer LoVo cells

更新时间：2026-01-09

- Over-expression of endothelin 3 suppresses migration and invasion in colorectal cancer LoVo cells
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 646-652(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Clinical Key-discipline Construction Program of China(国卫办医函〔2013〕544号)
- DOI：10.16016/j.1000-5404.201610226
  CLC：
- Received：31 October 2016，
  
  Published：2017-07
- 稿件说明：
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Shili Xiang, Qian Xiao, Tingxiu Xiang, et al. Over-expression of endothelin 3 suppresses migration and invasion in colorectal cancer LoVo cells[J]. Journal of Third Military Medical University, 2017, 39(7): 646-652.
DOI：

Shili Xiang, Qian Xiao, Tingxiu Xiang, et al. Over-expression of endothelin 3 suppresses migration and invasion in colorectal cancer LoVo cells[J]. Journal of Third Military Medical University, 2017, 39(7): 646-652. DOI： 10.16016/j.1000-5404.201610226.

摘要

Abstract

Objective

To determine the effect of endothelin 3 (EDN3) on the proliferation

migration and invasion in colorectal cancer cell line LoVo and investigate the underlying mechanism.

Methods

Real-time fluorescent quantitative PCR was used to detect the expression level of EDN3 in colorectal cancer tissues and para-cancerous tissues (

=15). Semi-quantitative PCR was adopted to detect the mRNA expression of EDN3 and endothelin receptor B (EDNRB) in the colorectal cancer cell lines CaCO2

LoVo and HT-29. The recombinant plasmid pCDH-CMV-MCS-EF1-Puro-EDN3 was packaged into lentivirus and then infected into the LoVo cells. MTS assay

colony formation assay

wound healing assay

Transwell migration and invasion assay were conducted to analyze the changes of cells in the proliferation

colony formation

migration and invasion. Western blotting was used to detect the protein expression changes of p-ERK1/2 and t-ERK1/2 in the LoVo cells.

Results

The expression of EDN3 were down-regu

lated in colorectal cancer tissues and cell lines. The recombinant lentiviral vectors which express EDN3 gene were successfully infected into LoVo cells. MTS assay and colony formation assay showed that there were no changes in the proliferation and colony formation between the LoVo cells with the lentiviral infection and those with blank vector (

0.05). Wound healing test and Transwell assay revealed that the migration and invasion ability were apparently reduced in the experimental cells compared to the control cells (

0.01). Western blot assay showed that the protein level of p-ERK1/2 was decreased after the infection (

0.01).

Conclusion

The expression of EDN3 gene is generally down-regulated in colorectal cancer tissues and cell lines. EDN3 over-expression inhibits the migration and invasion in colorectal cancer LoVo cells

which may be through ERK1/2 signaling pathway.

关键词

Keywords

references

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