Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium

Qian Xu; Jiajia Yang; Zhihua Liu; Rui Liu; Jianping An; Xia Zhou; Faming Chen; Manjing Deng

doi:10.16016/j.1000-5404.201610220

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Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium

更新时间：2026-01-09

- Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 616-621(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the Opening Project of State Key Laboratory of Military Stomatology(2014KA04)
- DOI：10.16016/j.1000-5404.201610220
  CLC：
- Received：30 October 2016，
  
  Published：2017-07
- 稿件说明：
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Qian Xu, Jiajia Yang, Zhihua Liu, et al. Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium[J]. Journal of Third Military Medical University, 2017, 39(7): 616-621.
DOI：

Qian Xu, Jiajia Yang, Zhihua Liu, et al. Effect of periodontitis under hypoxia on expression of HIF-1α and MMP2 in rat periodontium[J]. Journal of Third Military Medical University, 2017, 39(7): 616-621. DOI： 10.16016/j.1000-5404.201610220.

摘要

Abstract

Objective

To investigate the expression of hypoxia-inducible factor-1α (HIF-1α) and matrix metalloproteinase 2 (MMP2) in the periodontal tissues of rats with periodontitis under hypoxia

and explore the relationship of HIF-1α and MMP2 expression with periodontitis.

Methods

Forty healthy adult rats were randomly divided into 4 groups: normoxia group

normoxic periodontitis group

hypoxia group (simulating 5 000 m above sea level) and hypoxic periodontitis group (

=10 for each group). Gingival index

plaque index

depth of probing and alveolar bone resorption were measured respectively. HE staining was used to observe periodontal inflammation in the periodontal tissue sections. Immunohistochemical assay and Western blotting were employed to detect the expression levels of HIF-1α and MMP2 in the periodontal tissues.

Results

Clinical indicators of periodontitis and the results of HE staining showed that the periodontal inflammation of the hypoxic periodontitis group was the most severe. Compared with the 2 normoxic groups

hypoxia promoted the expression of HIF-1α and MMP2 in the periodontal tissues in the 2 hypoxic groups (

0.05). The expression of HIF-1α and MMP2 was significantly higher in periodontitis groups than in the non-periodontitis groups (

0.05)

with the highest levels in the hypoxic periodontitis group (

0.05).

Conclusion

Hypoxia promotes the expression levels of HIF-1α and MMP2 in periodontitis and accelerates its progression.

关键词

Keywords

references

冯秀娟高海拔地区牙周疾病就诊原因分析高原医学杂志 2002 12 2 26 27 10.3969/j.issn.1007-3809.2002.02.011 冯秀娟.高海拔地区牙周疾病就诊原因分析[J].高原医学杂志, 2002, 12(2): 26-27.DOI: 10.3969/j.issn.1007-3809.2002.02.011

Feng X J Analysis of the causes of periodontal diseases in high altitude area Journal of High Altitude Medicine 2002 12 2 26 27 10.3969/j.issn.1007-3809.2002.02.011 Feng X J. Analysis of the causes of periodontal diseases in high altitude area[J]. Journal of High Altitude Medicine, 2002, 12(2): 26-27.DOI: 10.3969/j.issn.1007-3809.2002.02.011

Abdollahi H Harris L J Zhang P The role of hypoxia in stem cell differentiation and therapeutics J Surg Res 2011 165 1 112 117 10.1016/j.jss.2009.09.057 Abdollahi H, Harris L J, Zhang P, et al. The role of hypoxia in stem cell differentiation and therapeutics[J]. J Surg Res, 2011, 165(1): 112-117. DOI:10.1016/j.jss.2009.09.057

Campos K Gomes C C Farias L C DNA Methylation of MMP9 Is Associated with High Levels of MMP-9 Messenger RNA in Periapical Inflammatory Lesions J Endod 2016 42 1 127 130 10.1016/j.joen.2015.10.002 Campos K, Gomes C C, Farias L C, et al. DNA Methylation of MMP9 Is Associated with High Levels of MMP-9 Messenger RNA in Periapical Inflammatory Lesions[J]. J Endod, 2016, 42(1): 127-130. DOI: 10.1016/j.joen.2015.10.002

Bae W J Shin M R Kang S K HIF-2 Inhibition Supresses Inflammatory Responses and Osteoclastic Differentiation in Human Periodontal Ligament Cells J Cell Biochem 2015 116 7 1241 1255 10.1002/jcb.25078 Bae W J, Shin M R, Kang S K, et al. HIF-2 Inhibition Supresses Inflammatory Responses and Osteoclastic Differentiation in Human Periodontal Ligament Cells[J]. J Cell Biochem, 2015, 116(7): 1241-1255. DOI:10.1002/jcb.25078

Yu X J Xiao C J Du Y M Effect of hypoxia on the expression of RANKL/OPG in human periodontal ligament cells in vitro Int J Clin Exp Pathol 2015 8 10 12929 12935 https://www.ncbi.nlm.nih.gov/pubmed/26722486 Yu X J, Xiao C J, Du Y M, et al. Effect of hypoxia on the expression of RANKL/OPG in human periodontal ligament cells in vitro[J]. Int J Clin Exp Pathol, 2015, 8(10): 12929-12935

Fischer R G Klinge B Clinical and histological evaluation of ligature-induced periodontitis in the domestic ferret J Clin Periodontol 1994 21 4 230 239 10.1111/j.1600-051x.1994.tb00311.x Fischer R G, Klinge B. Clinical and histological evaluation of ligature-induced periodontitis in the domestic ferret[J]. J Clin Periodontol, 1994, 21(4): 230-239. DOI:10.1111/j.1600-051x.1994.tb00311.x

Loe H Silness J Periodontal Disease in Pregnancy: Prevalence and severity Acta Odontol Scand 1963 21 533 551 10.3109/00016356309011240 Loe H, Silness J. Periodontal Disease in Pregnancy: Prevalence and severity[J]. Acta Odontol Scand, 1963, 21:533-551. DOI:10.3109/00016356309011240

Llavaneras A Ramamurthy N S Heikkil P A combination of a chemically modified doxycycline and a bisphosphonate synergistically inhibits endotoxin-induced periodontal breakdown in rats J Periodontol 2001 72 8 1069 1077 10.1902/jop.2001.72.8.1069 Llavaneras A, Ramamurthy N S, Heikkil P, et al. A combination of a chemically modified doxycycline and a bisphosphonate synergistically inhibits endotoxin-induced periodontal breakdown in rats[J]. J Periodontol, 2001, 72(8): 1069-1077. DOI:10.1902/jop.2001.72.8.1069

Gölz L Memmert S Rath-Deschner B Hypoxia and P. gingivalis synergistically induce HIF-1 and NF-κB activation in PDL cells and periodontal diseases Mediators Inflamm 2015 2015 438085 10.1155/2015/438085 Gölz L, Memmert S, Rath-Deschner B, et al. Hypoxia and P. gingivalis synergistically induce HIF-1 and NF-κB activation in PDL cells and periodontal diseases[J]. Mediators Inflamm, 2015, 2015: 438085. DOI:10.1155/2015/438085

Li K Dong S G Zhang H X Expression of RUNX2 and MDM21 in rats with periodontitis under chronic intermittent hypoxia Asian Pac J Trop Med 2016 9 8 781 785 10.1016/j.apjtm.2016.06.002 Li K, Dong S G, Zhang H X, et al. Expression of RUNX2 and MDM21 in rats with periodontitis under chronic intermittent hypoxia[J]. Asian Pac J Trop Med, 2016, 9(8): 781-785. DOI:10.1016/j.apjtm.2016.06.002

Schipani E Maes C Carmeliet G Regulation of osteogenesis-angiogenesis coupling by HIFs and VEGF J Bone Miner Res 2009 24 8 1347 1353 10.1359/jbmr.090602 Schipani E, Maes C, Carmeliet G, et al. Regulation of osteogenesis-angiogenesis coupling by HIFs and VEGF[J]. J Bone Miner Res, 2009, 24(8): 1347-1353. DOI:10.1359/jbmr.090602

Bullen J W Tchernyshyov I Holewinski R J Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1 Sci Signal 2016 9 430 ra56 10.1126/scisignal.aaf0583 Bullen J W, Tchernyshyov I, Holewinski R J, et al. Protein kinase A-dependent phosphorylation stimulates the transcriptional activity of hypoxia-inducible factor 1[J]. Sci Signal, 2016, 9(430): ra56. DOI:10.1126/scisignal.aaf0583

Li J P Li F Y Xu A Lipopolysaccharide and hypoxia-induced HIF-1 activation in human gingival fibroblasts J Periodontol 2012 83 6 816 824 10.1902/jop.2011.110458 Li J P, Li F Y, Xu A, et al. Lipopolysaccharide and hypoxia-induced HIF-1 activation in human gingival fibroblasts[J]. J Periodontol, 2012, 83(6): 816-824. DOI:10.1902/jop.2011.110458

Song Z C Zhou W Shu R Hypoxia induces apoptosis and autophagic cell death in human periodontal ligament cells through HIF-1α pathway Cell Prolif 2012 45 3 239 248 10.1111/j.1365-2184.2012.00810.x Song Z C, Zhou W, Shu R, et al. Hypoxia induces apoptosis and autophagic cell death in human periodontal ligament cells through HIF-1α pathway[J]. Cell Prolif, 2012, 45(3): 239-248. DOI:10.1111/j.1365-2184.2012.00810.x

Thornton R D Lane P Borghaei R C Interleukin 1 induces hypoxia-inducible factor 1 in human gingival and synovial fibroblasts Biochem J 2000 350 Pt1 307 312 10.1042/0264-6021:3500307 Thornton R D, Lane P, Borghaei R C, et al. Interleukin 1 induces hypoxia-inducible factor 1 in human gingival and synovial fibroblasts[J]. Biochem J, 2000, 350(Pt1): 307-312. DOI:10.1042/0264-6021:3500307

Tang L Pei H Yang Y The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-β1 pathway Sci Rep 2016 6 29975 10.1038/srep29975 Tang L, Pei H, Yang Y, et al. The inhibition of calpains ameliorates vascular restenosis through MMP2/TGF-β1 pathway[J]. Sci Rep, 2016, 6: 29975. DOI: 10.1038/srep29975

Ebersole J L Kirakodu S S Novak M J Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging Front Immunol 2016 7 272 10.3389/fimmu.2016.00272 Ebersole J L, Kirakodu S S, Novak M J, et al. Transcriptome Analysis of B Cell Immune Functions in Periodontitis: Mucosal Tissue Responses to the Oral Microbiome in Aging[J]. Front Immunol, 2016, 7:272. DOI: 10.3389/fimmu.2016.00272

Deppe J Popp T Egea V Impairment of hypoxia-induced HIF-1α signaling in keratinocytes and fibroblasts by sulfur mustard is counteracted by a selective PHD-2 inhibitor Arch Toxicol 2016 90 5 1141 1150 10.1007/s00204-015-1549-y Deppe J, Popp T, Egea V, et al. Impairment of hypoxia-induced HIF-1α signaling in keratinocytes and fibroblasts by sulfur mustard is counteracted by a selective PHD-2 inhibitor[J]. Arch Toxicol, 2016, 90(5): 1141-1150. DOI: 10.1007/s00204-015-1549-y

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