Inhibitory effect of psoralen on osteoclast formation and its underlying mechanism <italic style="font-style: italic">in vitro</italic>

Wenjuan Zhang; Baoping Xie; Weijuan Li; Jingping Li; Guoxing Gan; Jie Zhang

doi:10.16016/j.1000-5404.201610207

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Inhibitory effect of psoralen on osteoclast formation and its underlying mechanism in vitro

更新时间：2026-01-09

- Inhibitory effect of psoralen on osteoclast formation and its underlying mechanism in vitro
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 641-645(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  General Program of National Natural Science Foundation of China(81273816)
- DOI：10.16016/j.1000-5404.201610207
  CLC：
- Received：23 October 2016，
  
  Published：2017-07
- 稿件说明：
移动端阅览
Wenjuan Zhang, Baoping Xie, Weijuan Li, et al. Inhibitory effect of psoralen on osteoclast formation and its underlying mechanism in vitro[J]. Journal of Third Military Medical University, 2017, 39(7): 641-645.
DOI：

Wenjuan Zhang, Baoping Xie, Weijuan Li, et al. Inhibitory effect of psoralen on osteoclast formation and its underlying mechanism in vitro[J]. Journal of Third Military Medical University, 2017, 39(7): 641-645. DOI： 10.16016/j.1000-5404.201610207.

摘要

Abstract

Objective

To determine the effect of psoralen on osteoclast differentiation and on the regulation of ER-alpha and IL-17R in the osteoclasts in order to preliminary clarify the anti-osteoporosis effect of psoralen.

Methods

In order to induce the differentiation of to osteoclasts

mouse macrophage RAW264.7 cells were divided into 5 groups

and treated respectively with receptor activator for nuclear factor-κB ligand (RANKL)

RANKL+estradiol (positive control)

and RANKL+psoralen at 3 different concentrations (10

5 and 2.5 μmol/L). In 5 d after the treatment

tartrate-resistant acid phosphatase (TRAP) staining and bone resorption essay were used to identify the inhibitory effects of psoralen on the differentiation of osteoclasts. Transcriptional and protein concentration levels of matrix metallopeptidase 9 (MMP-9)

TRAP and Cathepsin K were detected by RT-PCR and ELISA to clarify the activity of osteoclasts

and the expression of IL-17R and estrogen receptor-α (ER-α) was also detected for the role of psoralen in the process.

Results

Compared with control cells

high-

middle-and low-dose psoralen significantly inhibited the numbers of osteoclasts and osteoclast-mediated bone resorption

as well as the gene expression of Cathepsin K

TRAP and IL-17R (

0.05

0.01)

while markedly enhanced the expression of ER-α (

0.01). The 3 doses of psoralen also reduced the MMP-9 expression significantly (

0.05). Compared with the control group

the protein level of Cathepsin K was very significantly decreased in the high-and middle-dose psoralen groups (

0.01). The treatment of psoralen at 3 doses also markedly reduced the protein contents of TRAP

MMP-9 and IL-17R (

0.05

0.01)

and promoted that of ER-α (

0.01).

Conclusion

Psoralen remarkably inhibits the differentiation of osteoclasts and the function of osteolysis at the same time

which might be due to its enhancing the expression of ER and suppressing that of IL-17R.

关键词

Keywords

references

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⁰

Inhibitory effect of psoralen on osteoclast formation and its underlying mechanism in vitro

DOI：10.16016/j.1000-5404.201610207

摘要

Abstract

关键词

Keywords

references