Expression profile and function of serine hydroxymethyl transferase 2 during liver regeneration in mice

Fangchao Yuan; Jianping Gong

doi:10.16016/j.1000-5404.201610167

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Expression profile and function of serine hydroxymethyl transferase 2 during liver regeneration in mice

更新时间：2026-01-09

- Expression profile and function of serine hydroxymethyl transferase 2 during liver regeneration in mice
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 675-679(2017)
- 作者机构：
- 作者简介：
- 基金信息：
- DOI：10.16016/j.1000-5404.201610167
  CLC：
- Received：25 October 2016，
  
  Published：2017-07
- 稿件说明：
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Fangchao Yuan, Jianping Gong. Expression profile and function of serine hydroxymethyl transferase 2 during liver regeneration in mice[J]. Journal of Third Military Medical University, 2017, 39(7): 675-679.
DOI：

Fangchao Yuan, Jianping Gong. Expression profile and function of serine hydroxymethyl transferase 2 during liver regeneration in mice[J]. Journal of Third Military Medical University, 2017, 39(7): 675-679. DOI： 10.16016/j.1000-5404.201610167.

摘要

Abstract

Objective

To determine the expression profile of serine hydroxymethyl transferase 2 (SHMT2) during mouse liver regeneration and investigate whether it exerts promoting effect in the process.

Methods

A total of 120 SPF C57BL/6 mice were randomly assigned to 2/3 partial hepatectomy (2/3PH)

sham operation

siSHMT2 and control groups (normal saline). The liver tissue and serum samples were collected at 1

7 and 9 d after the surgery with 6 mice in each time point. The expression profiles of SHMT2 and its downstream molecule glycine dehydrogenase (GLDC)

and the serum levels of alanine transaminase (ALT) and aspartate transaminase (AST) were detected by qPCR

Western blotting and immunohistochemical assay respectively. The effect of siSHMT2 was determined by observing fluorescent signals

detecting the expression of SHMT2 and proliferating cell nuclear antigen (PCNA) and serum levels of ALT and AST.

Results

The expression levels of SHMT2 and GLDC reached highest in 5 and 7 d after operation

with the mRNA amount of SHMT2 increased by 1.63 times at day 5 than day 1 (

0.05). When compared with the 2/3PH group

SHMT2 interference resulted in the lower liver weight at 5 d (0.63±0.11

0.79±0.13 g

0.05)

lower liver regeneration [(31.33±1.92)%

(36.37±2.21) %

]

and liver index [(3.13±0.29)%

(3.76±0.44)%

]

and decreased positive rate of PCNA [(39.0±3.2)%

(53.6±2.3)%

0.05

]

while increased serum levels of AST (403.41±68.63

140.09±32.85 U/L

0.05) and ALT (154.15±16.49

70.00±9.52 U/L

0.05).

Conclusion

SHMT2 is highly expressed in the late stage of liver regeneration

and promotes liver regeneration

which might be through its enhancing liver tolerance to ischemia and hypoxia and promoting adenosine triphosphate level.

关键词

Keywords

references

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