Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells

Nian WU; Ping ZHANG; Dongdong TIAN; Ranxi ZHANG; Qianzhao CHEN; Baicheng HE; Liangjun YIN

doi:10.16016/j.1000-5404.201610139

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Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells

Basic Medicine | 更新时间：2026-01-09

- Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells
- Journal of Third Military Medical University Vol. 39, Issue 13, Pages: 1333-1338(2017)
- 作者机构：
  
  1.400010 重庆，重庆医科大学附属第二医院骨科
  2.400016 重庆，重庆市生物化学与分子药理学重点实验室
  3.400016 重庆，重庆医科大学药学院药理学教研室
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81572226)
- DOI：10.16016/j.1000-5404.201610139
  CLC： R34;R730.23;R738.1
- Received：21 October 2016，
  
  Revised：2016-12-07，
  
  Published：15 July 2017
- 稿件说明：
移动端阅览
Nian WU, Ping ZHANG, Dongdong TIAN, et al. Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells[J]. Journal of Third Military Medical University, 2017, 39(13): 1333-1338.
DOI：

Nian WU, Ping ZHANG, Dongdong TIAN, et al. Effects of all-trans-retinoic acid and bone morphogenetic protein 9 on proliferation and differentiation of human osteosarcoma cells[J]. Journal of Third Military Medical University, 2017, 39(13): 1333-1338. DOI： 10.16016/j.1000-5404.201610139.

摘要

目的

研究全反式维甲酸(all-trans-retinoic acid，ATRA)与骨形态发生蛋白9(bone morphogenetic proteins 9，BMP9) 对人骨肉瘤细胞增殖及分化的影响。

方法

利用四甲基偶氮唑蓝(MTT)法和流式细胞仪分析细胞增殖情况；蛋白质免疫印迹实验(Western blot)检测细胞增殖和成骨相关指标；半定量PCR及Western blot分析ATRA和ATRA与BMP9联用处理143B细胞株后对BMP9表达水平的影响。

结果

在人骨肉瘤143B细胞株中，与对照组相比，ATRA能明显抑制细胞增殖(

0.05)，并促使细胞周期阻滞在G

期(

0.05)，下调PCNA的蛋白水平，并呈浓度依赖性；ATRA能呈浓度依赖性增加晚期成骨指标OCN和OPN的蛋白水平(

0.05)；ATRA能上调BMP9的mRNA及蛋白水平，并呈浓度依赖性(

0.05)；单用BMP9并不能上调OCN和OPN表达，并促进其增殖(

0.05)，但与ATRA联用时，能够增强ATRA诱导的相关成骨指标表达和抗增殖作用(

0.05)。

结论

ATRA对143B细胞的增殖具有抑制作用并诱导其成骨分化，并可能恢复BMP9的成骨诱导能力。

Abstract

Objective

To determine the effects of all-trans-retinoic acid (ATRA) and bone morphogenetic protein 9 (BMP9) on the proliferation and osteogenic differentiation of human osteosarcoma (OS) cells.

Methods

MTT assay and flow cytometry were used to analyze the proliferation of the human OS cell line 143B. Western blot analysis was used to detect the proliferation-and osteogenesis-related markers. Semi-quantitative PCR and Western blot analysis were used to analyze the expression levels of BMP9 in 143B cells after treated with ATRA alone or combined with BMP9.

Results

In the 143B cells

ATRA significantly inhibited the proliferation of 143B cells (

0.05) compared with the untreated group

promoted cell cycle arrested in G1 phase (

0.05)

and reduced the pro

tein level of PCNA in

concentration-dependent manner. The protein levels of lately osteogenic makers OPN and OCN were up-regulated by ATRA treatment in

concentration-dependent manner (

0.05)

so were the mRNA and protein levels of endogenous BMP9 (

0.05). BMP9 alone failed to up-regulate the expression of OPN and OCN but promoted the proliferation of 143B cells (

0.05)

but when combined with ATRA

BMP9 enhanced the expression of osteogenic markers and anti-proliferation effect induced by ATRA (

0.05).

Conclusion

ATRA inhibit the proliferation and induce osteogenic differentiation in 143B cells

and may restore the osteogenic capability of BMP9.

关键词

Keywords

references

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