Effect of Tmub1 on mitosis of regenerating hepatocytes in rats

Hangwei Fu; Jian Chen; Jianhua Xu; Guangyao Li; Ping Chen

doi:10.16016/j.1000-5404.201610134

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Effect of Tmub1 on mitosis of regenerating hepatocytes in rats

更新时间：2026-01-09

- Effect of Tmub1 on mitosis of regenerating hepatocytes in rats
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 603-607(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of the National Natural Science Foundation of China(81270523)
- DOI：10.16016/j.1000-5404.201610134
  CLC：
- Received：21 October 2016，
  
  Published：2017-07
- 稿件说明：
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Hangwei Fu, Jian Chen, Jianhua Xu, et al. Effect of Tmub1 on mitosis of regenerating hepatocytes in rats[J]. Journal of Third Military Medical University, 2017, 39(7): 603-607.
DOI：

Hangwei Fu, Jian Chen, Jianhua Xu, et al. Effect of Tmub1 on mitosis of regenerating hepatocytes in rats[J]. Journal of Third Military Medical University, 2017, 39(7): 603-607. DOI： 10.16016/j.1000-5404.201610134.

摘要

Abstract

Objective

To determine the effect of transmembrane and ubiquitin like domain containing 1 (Tmub1) on the mitosis in regenerating hepatocytes and its interaction with securin.

Methods

Totally 40 SD female rats were randomly divided into 8 groups according to the time points after 70% partial hepatectomy (PH). Regenerating liver samples were collected in 0

96 and 168 h after PH. RT-qPCR and Western blotting were conducted to measure the mRNA and protein expression patterns of Tmub1 and securin in the process of liver regeneration. BRL-3A cells with stable Tmub1 over-expression and knockdown were established respectively by corresponding lentiviral vectors. Prometaphase arrest was accomplished by nocodazole treatment

and cell mitosis was observed by immunofluorescence staining in the cells with Tmub1 over-expression and knockdown

and control cells respectively. Securin ubiquitylation levels were determined by immunoprecipitation and Western blotting in the 3 groups of cells.

Results

The mRNA and protein levels of Tmub1 reached the highest at 24 and 48 h after PH. Securin mRNA level also peaked at 48 h after PH

but its protein level was the lowest at this time point. Compared to negative control group

Tmub1 over-expression slowed down the mitosis process and significantly lower ubiquitylation level of secur

in (

0.01)

while Tmub1 interference improved the securin ubiquitylation level (

0.01).

Conclusion

Tmub1 inhibits hepatocyte mitosis by suppressing securin ubiquitylation in regenerating hepatocytes from rats after PH.

关键词

Keywords

references

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