High phosphorus induces calcification of cultured vascular smooth muscle cells by activating Toll-like receptor 4/nuclear factor-κB signaling pathway

Haidao Zhang; Tangli Xiao; Yanlin Yu; Liang Liu; Yong Liu; Changling Zheng; Ling Nie; Jinghong Zhao

doi:10.16016/j.1000-5404.201610128

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High phosphorus induces calcification of cultured vascular smooth muscle cells by activating Toll-like receptor 4/nuclear factor-κB signaling pathway

更新时间：2026-01-09

- High phosphorus induces calcification of cultured vascular smooth muscle cells by activating Toll-like receptor 4/nuclear factor-κB signaling pathway
- Journal of Third Military Medical University Vol. 39, Issue 8, Pages: 731-736(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the National Natural Science Foundation for Young Scholars of China(81400747);the Key Project of Basic and Frontier Research Plan of Chongqing(CSTC2015jcyjBX0028)
- DOI：10.16016/j.1000-5404.201610128
  CLC：
- Received：19 October 2016，
  
  Published：30 April 2017
- 稿件说明：
移动端阅览
Haidao Zhang, Tangli Xiao, Yanlin Yu, et al. High phosphorus induces calcification of cultured vascular smooth muscle cells by activating Toll-like receptor 4/nuclear factor-κB signaling pathway[J]. Journal of Third Military Medical University, 2017, 39(8): 731-736.
DOI：

Haidao Zhang, Tangli Xiao, Yanlin Yu, et al. High phosphorus induces calcification of cultured vascular smooth muscle cells by activating Toll-like receptor 4/nuclear factor-κB signaling pathway[J]. Journal of Third Military Medical University, 2017, 39(8): 731-736. DOI： 10.16016/j.1000-5404.201610128.

摘要

Abstract

Objective

To investigate the role of Toll-like receptor 4 (TLR4)/nuclear factor-κB (NF-κB) signaling pathway in osteoblast-like differentiation and calcification of vascular smooth muscle cells (VSMCs) induced by high phosphate.

Methods

Cultured mouse VSMCs were exposed to high phosphorus and calcium deposition in the cells was observed with Alizarin red S staining. The effect of pyrrolidine dithiocarbamate (PDTC

20 μmol/L)

a NF-κB inhibitor

on osteogenic differentiation and calcification of the VSMCs with or without exposure to high phosphorus was assessed. The cells were transfected with a small interfering RNA (siRNA) targeting TLR4 or a control siRNA to test the effects of TLR4 knockdown on osteogenic differentiation and calcification of VSMCs exposed to high phosphorus. Western blotting was used to detect the expression of TLR4

p-NF-κB

SM22α

bone morphogenic protein 2 (BMP2) and runt-related transcription factor 2 (Runx2) in the cells with different treatments.

Results

Compared with the normal control cells

the VSMCs exposed to high phosphorus exhibited significantly increased calcium deposition (0.079±0.011

0.193±0.017

0.01) and expression levels of TLR4 (0.228±0.008

0.526±0.017

0.01)

p-NF-κB (0.271±0.003

0.454±0.008

0.01)

BMP2 and Runx2 but with a decreased expression of SM22α. Treatment of the high phosphorus-exposed cells with PDTC significantly decreased calcium deposition in the cells (0.121±0.013

0.217±0.031

0.01)

lowered the expression of p-NF-κB (0.094±0.006

0.380±0.009

0.01)

BMP2 and Runx2

and up-regulated the expression of SM22α. Transfection of the high phosphorus-exposed VSMCs with the TLR4 siRNA significantly suppressed the expression of TLR4 (0.117±0.047

0.377±0.012

0.01)

decreased the expression of p-NF-κB (0.264±0.013

0.601±0.027

0.01)

BMP2 (

0.01) and Runx2 (

0.01)

and increased the expression of SM22α (

0.01).

Conclusion

High phosphorus can induce osteoblast differentiation and calcification in mouse VSMCs

in vitro

by activating the TLR4/NF-κB signaling pathway.

关键词

Keywords

references

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