Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells

Weiwei Yi; Yafeng Wen; Xi Liu; He Ye; Bo Liu

doi:10.16016/j.1000-5404.201610083

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Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells

更新时间：2026-01-09

- Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells
- Journal of Third Military Medical University Vol. 39, Issue 8, Pages: 755-759(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81572202)
- DOI：10.16016/j.1000-5404.201610083
  CLC：
- Received：14 October 2016，
  
  Published：30 April 2017
- 稿件说明：
移动端阅览
Weiwei Yi, Yafeng Wen, Xi Liu, et al. Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells[J]. Journal of Third Military Medical University, 2017, 39(8): 755-759.
DOI：

Weiwei Yi, Yafeng Wen, Xi Liu, et al. Effect of nuclear factor-κB inhibitor on expression of inflammatory factors in lipopolysaccharide-stimulated degenerative human nucleus pulposus cells[J]. Journal of Third Military Medical University, 2017, 39(8): 755-759. DOI： 10.16016/j.1000-5404.201610083.

摘要

Abstract

Objective

To investigate the changes in the expression of nuclear factor-κB (NF-κB) and inflammatory cytokines in degenerative human intervertebral disc nucleus pulposus cells in response to lipopolysaccharide (LPS) stimulation.

Methods

Cultured degenerative human nucleus pulposus cells were treated with PBS (control)

LPS (500 μg/mL)

or a NF-κB inhibitor (BAY11-7082) plus LPS (500 μg/mL). The levels of tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) in the culture medium were detected with enzyme-linked immunosorbent assay (ELISA). The expression and localization of p65 in the nucleus pulposus cells were detected with immunofluorescence assay

and the cellular expression of TNF-α

IL-1β

NF-κB-binding protein p65 and phosphorylated p65 (p-p65) were detected with Western blotting; Real-time PCR was used to detect the expression of TNF-α and IL-1β mRNA in the cells.

Results

LPS stimulation significantly increased the nuclear translocation of p65 and enhanced p-p65 expression in cultured nucleus pulposus cells. LPS stimulation of the nucleus pulposus cells caused sig

nificant increases in the levels of TNF-α and IL-1β both in the culture medium (

0.05) and in the cells (

0.05). Compared with LPS-exposed cells

the cells treated with BAY11-7082 plus LPS showed a significant decrease in p65 translocation and p-p65 expression with also decreased levels of TNF-α and IL-1β in both the culture medium (

0.05) and in the cells (

0.05).

Conclusion

LPS stimulation can promote the expression of TNF-α and IL-1β by activating NF-κB signaling pathway in human degenerative nucleus pulposus cells

and inhibition of NF-κB signaling pathway can significantly reduce the expression of these inflammatory factors.

关键词

Keywords

references

Le Maitre C L Binch A L Thorpe A A Degeneration of the intervertebral disc with new approaches for treating low back pain J Neurosurg Sci 2015 59 1 47 61 http://www.minervamedica.it/en/journals/neurosurgical-sciences/article.php?cod=R38Y2015N01A0047 Le Maitre C L, Binch A L, Thorpe A A, et al. Degeneration of the intervertebral disc with new approaches for treating low back pain[J]. J Neurosurg Sci, 2015, 59(1): 47-61.

Hayden M S Ghosh S Shared principles in NF-kappaB signaling Cell 2008 132 3 344 362 10.1016/j.cell.2008.01.020 Hayden M S, Ghosh S. Shared principles in NF-kappaB signaling[J]. Cell, 2008, 132(3): 344-362. DOI:10.1016/j.cell.2008.01.020.

Lee J I Burckart G J Nuclear factor kappa B: important transcription factor and therapeutic target J Clin Pharmacol 1998 38 11 981 993 10.1177/009127009803801101 Lee J I, Burckart G J. Nuclear factor kappa B: important transcription factor and therapeutic target[J]. J Clin Pharmacol, 1998, 38(11): 981-993. DOI:10.1177/0091270098 03801101.

Nasto L A Seo H Y Robinson A R ISSLS prize winner: inhibition of NF-κB activity ameliorates age-associated disc degeneration in a mouse model of accelerated aging Spine 2012 37 21 1819 1825 10.1097/BRS.0b013e31824ee8f7 Nasto L A, Seo H Y, Robinson A R, et al. ISSLS prize winner: inhibition of NF-κB activity ameliorates age-associated disc degeneration in a mouse model of accelerated aging[J]. Spine, 2012, 37(21): 1819-1825. DOI:10.1097/BRS.0b013e31824ee8f7.

Pfirrmann C W Metzdorf A Zanetti M Magnetic resonance classification of lumbar intervertebral disc degeneration Spine 2001 26 17 1873 1878 10.1097/00007632-200109010-00011 Pfirrmann C W, Metzdorf A, Zanetti M, et al. Magnetic resonance classification of lumbar intervertebral disc degeneration[J]. Spine, 2001, 26(17): 1873-1878. DOI:10.1097/00007632-200109010-00011.

郭健行黄桂成椎间盘退变影响因素研究进展国际骨科学杂志 2008 29 1 46 48 10.3969/j.issn.1673-7083.2008.01.018 郭健行, 黄桂成.椎间盘退变影响因素研究进展[J].国际骨科学杂志, 2008, 29(1): 46-48. DOI:10.3969/j.issn.1673-7083.2008.01.018.

Guo J X Huang G C Progress in study on the influence factors of intervertebral disc degeneration Intern J Orthop 2008 29 1 46 48 10.3969/j.issn.1673-7083.2008.01.018 Guo J X, Huang G C. Progress in study on the influence factors of intervertebral disc degeneration[J]. Intern J Orthop, 2008, 29(1):46-48.DOI:10.3969/j.issn.1673-7083.2008.01.018.

Kepler C K Ponnappan R K Tannoury C A The molecular basis of intervertebral disc degeneration Spine J 2013 13 3 318 330 10.1016/j.spinee.2012.12.003 Kepler C K, Ponnappan R K, Tannoury C A, et al. The molecular basis of intervertebral disc degeneration[J]. Spine J, 2013, 13(3): 318-330. DOI:10.1016/j.spinee.2012.12.003.

杜伟申勇李宝俊细胞因子IL-1a、IL-6、TNF-a、MMP3与颈椎间盘退变机制的相关性研究中国矫形外科杂志 2012 20 3 259 262 10.3977/j.issn.1005-8478.2012.03.20 杜伟, 申勇, 李宝俊, 等.细胞因子IL-1a、IL-6、TNF-a、MMP3与颈椎间盘退变机制的相关性研究[J].中国矫形外科杂志, 2012, 20(3): 259-262. DOI:10.3977/j.issn.1005-8478.2012.03.20.

Du W Shen Y Li B J correlation between inflammatory cytokines IL-1α, IL-6, TNF-α and MMP3 levels versus cervical intervertebral disc degeneration mechanism Orthopedic Journal of China 2012 20 3 259 262 10.3977/j.issn.1005-8478.2012.03.20 Du W, Shen Y, Li B J, et al.correlation between inflammatory cytokines IL-1α, IL-6, TNF-α and MMP3 levels versus cervical intervertebral disc degeneration mechanism[J]. Orthopedic Journal of China, 2012, 20(3): 259-262.DOI:10.3977/j.issn.1005-8478.2012.03.20.

刘浠易威威温亚枫锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化第三军医大学学报 2016 38 18 2077 2081 10.16016/j.1000-5404.201602105 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201602105 刘浠, 易威威, 温亚枫, 等.锌指蛋白A20及其相关炎症因子在椎间盘髓核细胞退变前后的表达变化[J].第三军医大学学报, 2016, 38(18): 2077-2081.DOI:10.16016/j.1000-5404.201602105.

Liu X Yi W W Wen Y F Expression of zinc finger protein a20 and related inflammatory factors in nucleus pulposus cells in degenerative intervertebral disc J Third Mil Med Univ 2016 38 18 2077 2081 10.16016/j.1000-5404.201602105 http://aammt.tmmu.edu.cn/oa/DArticle.aspx?type=view&id=201602105 Liu X, Yi W W, Wen Y F, et al. Expression of zinc finger protein a20 and related inflammatory factors in nucleus pulposus cells in degenerative intervertebral disc[J]. J Third Mil Med Univ, 2016, 38(18): 2077-2081. DOI:10.16016/j.1000-5404.201602105.

Podichetty V K The aging spine: the role of inflammatory mediators in intervertebral disc degeneration Cell Mol Biol (Noisy-le-grand) 2007 53 5 4 18 Podichetty V K. The aging spine: the role of inflammatory mediators in intervertebral disc degeneration[J]. Cell Mol Biol (Noisy-le-grand), 2007, 53(5): 4-18.

钱胜君 , 张宁 , 陈维善 . 炎症因子与椎间盘退变 [J ] . 国际骨科学杂志 , 2009 , 30 ( 4 ): 247 - 248, 265 . DOI: 10.3969/j.issn.1673-7083.2009.04.012. 钱胜君, 张宁, 陈维善.炎症因子与椎间盘退变[J].国际骨科学杂志, 2009, 30(4): 247-248, 265. DOI:10.3969/j.issn.1673-7083.2009.04.012.

Qian S J , Zhang N , Chen W S . Inflammatory factor and intervertebral disc degeneration [J ] . Intern J Orthp , 2009 , 30 ( 4 ): 247 - 248, 265 .DOI: 10.3969/j.issn.1673-7083.2009.04.012. Qian S J, Zhang N, Chen W S. Inflammatory factor and intervertebral disc degeneration[J]. Intern J Orthp, 2009, 30(4): 247-248, 265.DOI:10.3969/j.issn.1673-7083.2009.04.012.

孙中仪田纪伟炎性因子与椎间盘退变的研究进展中华医学杂志 2013 93 41 3322 3324 10.3760/cma.j.issn.0376-2491.2013.41.023 孙中仪, 田纪伟.炎性因子与椎间盘退变的研究进展[J].中华医学杂志, 2013, 93(41): 3322-3324. DOI:10.3760/cma.j.issn.0376-2491.2013.41.023.

Sun Z Y Tian J W Research progress of inflammatory factors and intervertebral disc degeneration Natl Med J China 2013 93 41 3322 3324 10.3760/cma.j.issn.0376-2491.2013.41.023 Sun Z Y, Tian J W. Research progress of inflammatory factors and intervertebral disc degeneration[J]. Natl Med J China, 2013, 93(41): 3322-3324. DOI:10.3760/cma.j.issn.0376-2491.2013.41.023.

Wang J Markova D Anderson D G TNF-α and IL-1β promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc J Biol Chem 2011 286 46 39738 39749 10.1074/jbc.M111.264549 Wang J, Markova D, Anderson D G, et al. TNF-α and IL-1β promote a disintegrin-like and metalloprotease with thrombospondin type I motif-5-mediated aggrecan degradation through syndecan-4 in intervertebral disc[J]. J Biol Chem, 2011, 286(46): 39738-39749. DOI:10.1074/jbc.M111.264549.

Ponnappan R K Markova D Z Antonio P J An organ culture system to model early degenerative changes of the intervertebral disc Arthritis Res Ther 2011 13 5 R171 10.1186/ar3494 Ponnappan R K, Markova D Z, Antonio P J, et al. An organ culture system to model early degenerative changes of the intervertebral disc[J]. Arthritis Res Ther, 2011, 13(5): R171. DOI:10.1186/ar3494.

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