Analysis of PmpF polymorphism and distribution characteristics of <italic style="font-style: italic">Chlamydia trachomatis</italic> isolated from urinogenital specimens

Yueli Chang; Wei Wang; Ke Sun; Cunhui Zhang; Yanxia Zhang; Haixia Qiao; Yutuo Zhang

doi:10.16016/j.1000-5404.201610080

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Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens

更新时间：2026-01-09

- Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens
- Journal of Third Military Medical University Vol. 39, Issue 7, Pages: 653-659(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  Innovative Talents Program of Hebei North University(CXRC1317)
- DOI：10.16016/j.1000-5404.201610080
  CLC：
- Received：13 October 2016，
  
  Published：2017-07
- 稿件说明：
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Yueli Chang, Wei Wang, Ke Sun, et al. Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens[J]. Journal of Third Military Medical University, 2017, 39(7): 653-659.
DOI：

Yueli Chang, Wei Wang, Ke Sun, et al. Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens[J]. Journal of Third Military Medical University, 2017, 39(7): 653-659. DOI： 10.16016/j.1000-5404.201610080.

摘要

Abstract

Objective

To investigate the polymorphism of polymorphic membrane protein F gene (PmpF) of

Chlamydia trachomatis

(

C.t

) causing urinogenital tract infection and its relationship with clinical infection.

Methods

Clinical urinogenital specimens were collected from No. 252 Hospital of PLA from January 2013 to December 2014.

C.t

nucleic acid detection kit was used to screen positive specimens. Then the total DNA of obtained strain was extracted

and PmpF gene was amplified with nested PCR. The product was digested with 4 restriction endonucleases

and its genotype was determined with restriction fragment length polymorphism (RFLP). According to the results of PFLP

the representative strains were selected to sequence its PmpF gene to draw phylogenetic trees. Omp1 gene of representative strains were amplified and sequenced

and the results were compared with the reference strains to determine serotypes of these strains.

Chi

-square test was used analyzed the relationship of PmpF genotype with gender and age

and its clinical distribution.

Results

There were 178

C.t

specimens obtained from the departments of urology

gynecology and dermatology during the 2 years. Based on the PmpF gene amplified from the 117 specimens

there were 3 genotypes

accounting for 2.6%

36.8% and 60.7%

respectively

according to results of RFLP. Twenty-six strains were selected to amplify and sequence Omp1 gene

and the results showed that serotypes of these strains were D

and J. Statistical analysis showed that the PmpF genotype was correlated with age (

Chi

-square=0.008)

but not with gender (

Chi

-square=0.167).

Conclusion

There are 3 genotypes

and 7 serotypes of

C.t

strains infecting urinogenital tract in our region. The serotypes have no corresponding relationship with PmpF genotype

and the genotypes are correlated with age

but not with gender.

关键词

Keywords

references

Tamura K Stecher G Peterson D MEGA6: Molecular Evolutionary Genetics Analysis version 6.0 Mol Biol Evol 2013 30 12 2725 2729 10.1093/molbev/mst197 Tamura K, Stecher G, Peterson D, et al. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0[J]. Mol Biol Evol, 2013, 30(12):2725-2729.DOI: 10.1093/molbev/mst197

Ngandjio A Clerc M Fonkoua M C Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students J Med Microbiol 2004 53 Pt 1 47 50 10.1099/jmm.0.05333-0 Ngandjio A, Clerc M, Fonkoua M C, et al. Restriction endonuclease patterns of the omp1 gene of reference Chlamydia trachomatis strains and characterization of isolates from Cameroonian students[J]. J Med Microbiol, 2004, 53(Pt 1): 47-50. DOI:10.1099/jmm.0.05333-0

Lan J Walboomers J M Roosendaal R Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis J Clin Microbiol 1993 31 5 1060 1065 https://www.researchgate.net/publication/15100332_Direct_detection_and_genotyping_of_Chlamydia_trachomatis_in_cervical_scrapes_by_using_polymerase_chain_reaction_and_restriction_fragment_length_polymorphism_analysis Lan J, Walboomers J M, Roosendaal R, et al. Direct detection and genotyping of Chlamydia trachomatis in cervical scrapes by using polymerase chain reaction and restriction fragment length polymorphism analysis[J]. J Clin Microbiol, 1993, 31(5): 1060-1065.

Rebe K Lewis D Myer L A Cross Sectional Analysis of Gonococcal and Chlamydial Infections among Men-Who-Have-Sex-with-Men in Cape Town, South Africa PLoS ONE 2015 10 9 e0138315 10.1371/journal.pone.0138315 Rebe K, Lewis D, Myer L, et al. A Cross Sectional Analysis of Gonococcal and Chlamydial Infections among Men-Who-Have-Sex-with-Men in Cape Town, South Africa[J]. PLoS ONE, 2015, 10(9): e0138315. DOI:10.1371/journal.pone.0138315

Steen R Dallabetta G Genital ulcer disease control and HIV prevention J Clin Virol 2004 29 3 143 151 10.1016/j.jcv.2003.05.001 Steen R, Dallabetta G. Genital ulcer disease control and HIV prevention[J]. J Clin Virol, 2004, 29(3): 143-151. DOI:10.1016/j.jcv.2003.05.001

Gomes J P Bruno W J Borrego M J Recombination in the genome of Chlamydia trachomatis involving the polymorphic membrane protein C gene relative to ompA and evidence for horizontal gene transfer J Bacteriol 2004 186 13 4295 4306 10.1128/JB.186.13.4295-4306.2004 Gomes J P, Bruno W J, Borrego M J, et al. Recombination in the genome of Chlamydia trachomatis involving the polymorphic membrane protein C gene relative to ompA and evidence for horizontal gene transfer[J]. J Bacteriol, 2004, 186(13):4295-4306. DOI: 10.1128/JB.186.13.4295-4306.2004

Taylor B D Darville T Tan C The role of Chlamydia trachomatis polymorphic membrane proteins in inflammation and sequelae among women with pelvic inflammatory disease Infect Dis Obstet Gynecol 2011 2011 989762 10.1155/2011/989762 Taylor B D, Darville T, Tan C, et al. The role of Chlamydia trachomatis polymorphic membrane proteins in inflammation and sequelae among women with pelvic inflammatory disease[J]. Infect Dis Obstet Gynecol, 2011, 2011: 989762. DOI:10.1155/2011/989762

Mölleken K Schmidt E Hegemann J H Members of the Pmp protein family of Chlamydia pneumoniae mediate adhesion to human cells via short repetitive peptide motifs Mol Microbiol 2010 78 4 1004 1017 10.1111/j.1365-2958.2010.07386.x Mölleken K, Schmidt E, Hege mann J H. Members of the Pmp protein family of Chlamydia pneumoniae mediate adhesion to human cells via short repetitive peptide motifs[J ] . Mol Microbiol, 2010, 78(4):1004-1017. DOI:10.1111/j.1365-2958.2010.07386.x

Swanson K A Taylor L D Frank S D Chlamydia trachomatis polymorphic membrane protein D is an oligomeric autotransporter with a higher-order structure Infect Immun 2009 77 1 508 516 10.1128/IAI.01173-08 Swanson K A, Taylor L D, Frank S D, et al. Chlamydia trachomatis polymorphic membrane protein D is an oligomeric autotransporter with a higher-order structure[J]. Infect Immun, 2009, 77(1): 508-516. DOI:10.1128/IAI.01173-08

Gomes J P Nunes A Bruno W J Polymorphisms in the nine polymorphic membrane proteins of Chlamydia trachomatis across all serovars: evidence for serovar Da recombination and correlation with tissue tropism J Bacteriol 2006 188 1 275 286 10.1128/JB.188.1.275-286.2006 Gomes J P, Nunes A, Bruno W J, et al. Polymorphisms in the nine polymorphic membrane proteins of Chlamydia trachomatis across all serovars: evidence for serovar Da recombination and correlation with tissue tropism[J]. J Bacteriol, 2006, 188(1): 275-286. DOI:10.1128/JB.188.1.275-286.2006

Vandahl B B Pedersen A S Gevaert K The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029 BMC Microbiol 2002 2 36 https://www.researchgate.net/publication/11016859_The_expression_processing_and_localization_of_polymorphic_membrane_proteins_in_Chlamydia_pneumoniae_strain_CWL029 Vandahl B B, Pedersen A S, Gevaert K, et al. The expression, processing and localization of polymorphic membrane proteins in Chlamydia pneumoniae strain CWL029[J]. BMC Microbiol, 2002, 2:36.

常月立张彦霞乔海霞沙眼衣原体多态膜蛋白PmpF基因克隆及生物信息学分析中国病原生物学杂志 2015 10 9 786 791 10.13350/j.cjpb.150905 常月立, 张彦霞, 乔海霞, 等.沙眼衣原体多态膜蛋白PmpF基因克隆及生物信息学分析[J].中国病原生物学杂志, 2015, 10(9):786-791. DOI: 10.13350/j.cjpb.150905.

Chang Y L Zhang Y X Qiao H X Cloning and bioinformatic analysis of polymorphic membrane protein F of Chlamydia trachomatis Journal of Pathogen Biology 2015 10 9 786 791 10.13350/j.cjpb.150905 Chang Y L, Zhang Y X, Qiao H X, et al. Cloning and bioinformatic analysis of polymorphic membrane protein F of Chlamydia trachomatis [J].Journal of Pathogen Biology, 2015, 10(9):786-791. DOI: 10.13350/j.cjpb.150905

Morré S A Rozendaal L van Valkengoed I G Urogenital Chlamydia trachomatis serovars in men and women with a symptomatic or asymptomatic infection: an association with clinical manifestations J Clin Microbiol 2000 38 6 2292 2296 https://www.cabdirect.org/cabdirect/abstract/20002013753 Morré S A, Rozendaal L, van Valkengoed I G, et al. Urogenital Chlamydia trachomatis serovars in men and women with a symptomatic or asymptomatic infection: an association with clinical manifestations[J]. J Clin Microbiol, 2000, 38(6): 2292-2296.

Geisler W M Suchland R J Whittington W L The relationship of serovar to clinical manifestations of urogenital Chlamydia trachomatis infection Sex Transm Dis 2003 30 2 160 165 10.1097/00007435-200302000-00013 Geisler W M, Suchland R J, Whittington W L, et al. The relationship of serovar to clinical manifestations of urogenital Chlamydia trachomatis infection[J]. Sex Transm Dis, 2003, 30(2): 160-165. DOI:10.1097/00007435-200302000-00013

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Analysis of PmpF polymorphism and distribution characteristics of Chlamydia trachomatis isolated from urinogenital specimens

DOI：10.16016/j.1000-5404.201610080

摘要

Abstract

关键词

Keywords

references