SigB (Q225P) mutation promotes formation of <italic style="font-style: italic">Staphylococcus aureus</italic> biofilm

Hui Liu; Weilong Shang; Huagang Peng; Kun Zhou; Zhen Hu; Renjie Zhou; Xiancai Rao

doi:10.16016/j.1000-5404.201610077

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SigB (Q225P) mutation promotes formation of Staphylococcus aureus biofilm

更新时间：2026-01-09

- SigB (Q225P) mutation promotes formation of Staphylococcus aureus biofilm
- Journal of Third Military Medical University Vol. 39, Issue 8, Pages: 711-717(2017)
- 作者机构：
- 作者简介：
- 基金信息：
  
  the General Program of National Natural Science Foundation of China(81672071)
- DOI：10.16016/j.1000-5404.201610077
  CLC：
- Received：13 October 2016，
  
  Published：30 April 2017
- 稿件说明：
移动端阅览
Hui Liu, Weilong Shang, Huagang Peng, et al. SigB (Q225P) mutation promotes formation of Staphylococcus aureus biofilm[J]. Journal of Third Military Medical University, 2017, 39(8): 711-717.
DOI：

Hui Liu, Weilong Shang, Huagang Peng, et al. SigB (Q225P) mutation promotes formation of Staphylococcus aureus biofilm[J]. Journal of Third Military Medical University, 2017, 39(8): 711-717. DOI： 10.16016/j.1000-5404.201610077.

摘要

Abstract

Objective

To investigate the role of SigB (Q225P) in regulating biofilm formation of

Staphylococcus aureus

(

aureus

Methods

The biological characteristics of a clinically isolated

aureus

strain (XQ) and its mutant strain XQW obtained by passage were compared. The biofilm formation was assessed by crystal violet staining and laser confocal microscopy. Genome sequencing of the 2 strains was performed to compare the relevant genes and identify the mutation sites. Homologous recombination method was used to construct different mutant

aureus

strains

namely Newman-

sigB

(Q225P) with

sigB

(Q225P) gene replacement

Newman-△

sigB

with

sigB

deletion

and the complementary strains Newman-△

sigB

and Newman-△

sigB

(Q225P)

and biofilm formations of these strains were compared.

Results

XQW strain grew to form flocculent structures in liquid culture medium and showed a significantly greater ability of biofilm formation than its wild-type strain XQ as determined by crystal violet staining and laser confocal microscopy. Genome comparison suggested that a SigB (Q225P) mutation occurred in XQW strain. The constructed Newman-

sigB

(

Q225P) strain showed a much stronger ability of biofilm formation than wild-type Newman strain

a result consistent with the finding in XQW strain. The biofilm formation ability of Newman-△

sigB

(Q225P) strain was also stronger than that of Newman-△

sigB

strain.

Conclusion

SigB (Q225P) mutation promotes biofilm formation in

aureus

关键词

Keywords

references

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⁰

SigB (Q225P) mutation promotes formation of Staphylococcus aureus biofilm

DOI：10.16016/j.1000-5404.201610077

摘要

Abstract

关键词

Keywords

references